{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T00:14:34Z","timestamp":1772928874941,"version":"3.50.1"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2007,2,1]],"date-time":"2007-02-01T00:00:00Z","timestamp":1170288000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Beryllium (Be)-antigen presentation to Be-specific CD4+ T cells from the lungs of patients with chronic beryllium disease (CBD) results in T cell proliferation and TNF-\u03b1 secretion. We tested the hypothesis that Be-induced, CBD bronchoalveolar lavage (BAL) T cell, transcription-dependent, TNF-\u03b1 secretion was accompanied by specific transcription factor upregulation. After 6 h of Be stimulation, CBD BAL cells produced a median of 883 pg\/ml TNF-\u03b1 (range, 608\u20131,275 pg\/ml) versus 198 pg\/ml (range, 116\u2013245 pg\/ml) by unstimulated cells. After 12 h CBD BAL cells produced a median of 2,963 pg\/ml (range, 99\u20139,424 pg\/ml) TNF-\u03b1 versus 55 pg\/ml (range, 0\u2013454) by unstimulated cells. Using real-time RT-PCR, Be-stimulated TNF-\u03b1 production at 6 h was preceded by a 5-fold increase in TNF-\u03b1 pre-mRNA copy number:\u03b2-actin copy number (Be median ratio 0.21; unstimulated median ratio 0.04). The median ratio of mature TNF-\u03b1 mRNA:\u03b2-actin mRNA was upregulated 1.4-fold (Be median ratio 0.17; unstimulated median ratio 0.12). Be exposure in the presence of the transcription inhibitor pentoxifylline (PTX) decreased CBD BAL cell TNF-\u03b1 pre-mRNA levels &amp;gt; 60%, whereas treatment with the mRNA splicing inhibitor 2-aminopurine (2AP) decreased levels 40% relative to Be exposure alone. PTX treatment decreased mature TNF-\u03b1 mRNA levels 50% while 2AP decreased levels &amp;gt; 80%, relative to Be exposure alone. Beryllium exposure specifically upregulated transcription factors AP-1 and NF-\u03baB. The data suggest that Be exposure induces transcription-dependent TNF-\u03b1 production, potentially due to upregulation of specific transcription factors.<\/jats:p>","DOI":"10.1165\/rcmb.2006-0021tr","type":"journal-article","created":{"date-parts":[[2006,9,15]],"date-time":"2006-09-15T20:56:29Z","timestamp":1158353789000},"page":"191-200","source":"Crossref","is-referenced-by-count":13,"title":["Beryllium-Induced TNF-\u03b1 Production Is Transcription-Dependent in Chronic Beryllium Disease"],"prefix":"10.1093","volume":"36","author":[{"given":"Richard T.","family":"Sawyer","sequence":"first","affiliation":[{"name":"Robert H. Hollis Laboratory of Environmental and Occupational Health, Division of Environmental and Occupational Health Sciences, Department of Medicine, Division of Environmental and Occupational Health Sciences, and Integrated Department of Immunology, National Jewish Medical and Research Center; Department of Medicine, Division of Pulmonary Science and Critical Care Medicine, Department of Medicine, Department of Immunology, and Department of Preventive Medicine and Biometrics, University of Colorado Health Sciences Center, Denver, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew P.","family":"Fontenot","sequence":"additional","affiliation":[{"name":"Robert H. Hollis Laboratory of Environmental and Occupational Health, Division of Environmental and Occupational Health Sciences, Department of Medicine, Division of Environmental and Occupational Health Sciences, and Integrated Department of Immunology, National Jewish Medical and Research Center; Department of Medicine, Division of Pulmonary Science and Critical Care Medicine, Department of Medicine, Department of Immunology, and Department of Preventive Medicine and Biometrics, University of Colorado Health Sciences Center, Denver, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tristan A.","family":"Barnes","sequence":"additional","affiliation":[{"name":"Robert H. Hollis Laboratory of Environmental and Occupational Health, Division of Environmental and Occupational Health Sciences, Department of Medicine, Division of Environmental and Occupational Health Sciences, and Integrated Department of Immunology, National Jewish Medical and Research Center; Department of Medicine, Division of Pulmonary Science and Critical Care Medicine, Department of Medicine, Department of Immunology, and Department of Preventive Medicine and Biometrics, University of Colorado Health Sciences Center, Denver, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles E.","family":"Parsons","sequence":"additional","affiliation":[{"name":"Robert H. Hollis Laboratory of Environmental and Occupational Health, Division of Environmental and Occupational Health Sciences, Department of Medicine, Division of Environmental and Occupational Health Sciences, and Integrated Department of Immunology, National Jewish Medical and Research Center; Department of Medicine, Division of Pulmonary Science and Critical Care Medicine, Department of Medicine, Department of Immunology, and Department of Preventive Medicine and Biometrics, University of Colorado Health Sciences Center, Denver, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian C.","family":"Tooker","sequence":"additional","affiliation":[{"name":"Robert H. Hollis Laboratory of Environmental and Occupational Health, Division of Environmental and Occupational Health Sciences, Department of Medicine, Division of Environmental and Occupational Health Sciences, and Integrated Department of Immunology, National Jewish Medical and Research Center; Department of Medicine, Division of Pulmonary Science and Critical Care Medicine, Department of Medicine, Department of Immunology, and Department of Preventive Medicine and Biometrics, University of Colorado Health Sciences Center, Denver, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa A.","family":"Maier","sequence":"additional","affiliation":[{"name":"Robert H. Hollis Laboratory of Environmental and Occupational Health, Division of Environmental and Occupational Health Sciences, Department of Medicine, Division of Environmental and Occupational Health Sciences, and Integrated Department of Immunology, National Jewish Medical and Research Center; Department of Medicine, Division of Pulmonary Science and Critical Care Medicine, Department of Medicine, Department of Immunology, and Department of Preventive Medicine and Biometrics, University of Colorado Health Sciences Center, Denver, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"May M.","family":"Gillespie","sequence":"additional","affiliation":[{"name":"Robert H. Hollis Laboratory of Environmental and Occupational Health, Division of Environmental and Occupational Health Sciences, Department of Medicine, Division of Environmental and Occupational Health Sciences, and Integrated Department of Immunology, National Jewish Medical and Research Center; Department of Medicine, Division of Pulmonary Science and Critical Care Medicine, Department of Medicine, Department of Immunology, and Department of Preventive Medicine and Biometrics, University of Colorado Health Sciences Center, Denver, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. Brigitte","family":"Gottschall","sequence":"additional","affiliation":[{"name":"Robert H. Hollis Laboratory of Environmental and Occupational Health, Division of Environmental and Occupational Health Sciences, Department of Medicine, Division of Environmental and Occupational Health Sciences, and Integrated Department of Immunology, National Jewish Medical and Research Center; Department of Medicine, Division of Pulmonary Science and Critical Care Medicine, Department of Medicine, Department of Immunology, and Department of Preventive Medicine and Biometrics, University of Colorado Health Sciences Center, Denver, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lori","family":"Silveira","sequence":"additional","affiliation":[{"name":"Robert H. Hollis Laboratory of Environmental and Occupational Health, Division of Environmental and Occupational Health Sciences, Department of Medicine, Division of Environmental and Occupational Health Sciences, and Integrated Department of Immunology, National Jewish Medical and Research Center; Department of Medicine, Division of Pulmonary Science and Critical Care Medicine, Department of Medicine, Department of Immunology, and Department of Preventive Medicine and Biometrics, University of Colorado Health Sciences Center, Denver, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"Hagman","sequence":"additional","affiliation":[{"name":"Robert H. Hollis Laboratory of Environmental and Occupational Health, Division of Environmental and Occupational Health Sciences, Department of Medicine, Division of Environmental and Occupational Health Sciences, and Integrated Department of Immunology, National Jewish Medical and Research Center; Department of Medicine, Division of Pulmonary Science and Critical Care Medicine, Department of Medicine, Department of Immunology, and Department of Preventive Medicine and Biometrics, University of Colorado Health Sciences Center, Denver, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lee S.","family":"Newman","sequence":"additional","affiliation":[{"name":"Robert H. Hollis Laboratory of Environmental and Occupational Health, Division of Environmental and Occupational Health Sciences, Department of Medicine, Division of Environmental and Occupational Health Sciences, and Integrated Department of Immunology, National Jewish Medical and Research Center; Department of Medicine, Division of Pulmonary Science and Critical Care Medicine, Department of Medicine, Department of Immunology, and Department of Preventive Medicine and Biometrics, University of Colorado Health Sciences Center, Denver, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2007,2,1]]},"reference":[{"key":"2026030623364650100_bib1","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/S1567-5769(01)00177-1","volume":"2","year":"2002","journal-title":"Int Immunopharmacol"},{"key":"2026030623364650100_bib2","doi-asserted-by":"crossref","first-page":"1473","DOI":"10.1172\/JCI0215846","volume":"110","year":"2002","journal-title":"J Clin Invest"},{"key":"2026030623364650100_bib3","first-page":"242","volume":"262","year":"1999","journal-title":"Science"},{"key":"2026030623364650100_bib4","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.1056\/NEJM198904273201702","volume":"320","year":"1989","journal-title":"N Engl J Med"},{"key":"2026030623364650100_bib5","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/0091-6749(91)90300-D","volume":"88","year":"1991","journal-title":"J Allergy Clin Immunol"},{"key":"2026030623364650100_bib6","doi-asserted-by":"crossref","first-page":"1192","DOI":"10.1164\/ajrccm.164.7.2012123","volume":"164","year":"2001","journal-title":"Am J Respir Crit Care Med"},{"key":"2026030623364650100_bib7","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1165\/rcmb.2003-0336OC","volume":"31","year":"2004","journal-title":"Am J Respir Cell Mol Biol"},{"key":"2026030623364650100_bib8","first-page":"246","volume":"188","year":"1998","journal-title":"J Exp Med"},{"key":"2026030623364650100_bib9","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1016\/S1074-7613(02)00456-9","volume":"17","year":"2002","journal-title":"Immunity"},{"key":"2026030623364650100_bib10","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1146\/annurev.cb.09.110193.001533","volume":"9","year":"1993","journal-title":"Annu Rev Cell Biol"},{"key":"2026030623364650100_bib11"},{"key":"2026030623364650100_bib12","first-page":"192","volume":"110","year":"1991","journal-title":"Surgery"},{"key":"2026030623364650100_bib13","doi-asserted-by":"crossref","first-page":"1230","DOI":"10.1016\/S0006-291X(88)81271-3","volume":"155","year":"1988","journal-title":"Biochem Biophys Res Commun"},{"key":"2026030623364650100_bib14","doi-asserted-by":"crossref","first-page":"1479","DOI":"10.1164\/ajrccm\/139.6.1479","volume":"139","year":"1989","journal-title":"Am Rev Respir Dis"},{"key":"2026030623364650100_bib15","first-page":"788","volume":"65","year":"2002","journal-title":"Am J Respir Crit Care Med"},{"key":"2026030623364650100_bib16","first-page":"1","volume":"118","year":"1978","journal-title":"Am Rev Respir Dis"},{"key":"2026030623364650100_bib17","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1164\/ajrccm\/148.4_Pt_1.985","volume":"148","year":"1993","journal-title":"Am Rev Respir Dis"},{"key":"2026030623364650100_bib18","doi-asserted-by":"crossref","first-page":"1884","DOI":"10.1164\/ajrccm.156.6.9610040","volume":"156","year":"1997","journal-title":"Am J Respir Crit Care Med"},{"key":"2026030623364650100_bib19","first-page":"696","volume":"135","year":"1997","journal-title":"Am Rev Respir Dis"},{"key":"2026030623364650100_bib20","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1172\/JCI200318317","volume":"112","year":"2003","journal-title":"J Clin Invest"},{"key":"2026030623364650100_bib21","first-page":"249","volume":"143","year":"2002","journal-title":"Toxicology"},{"key":"2026030623364650100_bib22","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1165\/ajrcmb.10.5.8179912","volume":"10","year":"1994","journal-title":"Am J Respir Cell Mol Biol"},{"key":"2026030623364650100_bib23","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1165\/rcmb.2004-0090OC","volume":"31","year":"2004","journal-title":"Am J Respir Cell Mol Biol"},{"key":"2026030623364650100_bib24","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.1016\/j.jaci.2005.01.019","volume":"115","year":"2005","journal-title":"J Allergy Clin Immunol"},{"key":"2026030623364650100_bib25","doi-asserted-by":"crossref","first-page":"518","DOI":"10.4049\/jimmunol.158.1.518","volume":"158","year":"1997","journal-title":"J Immunol"},{"key":"2026030623364650100_bib26","doi-asserted-by":"crossref","first-page":"2747","DOI":"10.4049\/jimmunol.163.5.2747","volume":"163","year":"1999","journal-title":"J Immunol"},{"key":"2026030623364650100_bib27","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1183\/09031936.01.17304030","volume":"17","year":"2001","journal-title":"Eur Respir J"},{"key":"2026030623364650100_bib28","doi-asserted-by":"crossref","first-page":"1174","DOI":"10.1183\/09031936.02.02232001","volume":"20","year":"2002","journal-title":"Eur Respir J"},{"key":"2026030623364650100_bib29","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.tox.2005.10.016","volume":"218","year":"2006","journal-title":"Toxicology"},{"key":"2026030623364650100_bib30","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1016\/S0272-5231(02)00029-1","volume":"23","year":"2002","journal-title":"Clin Chest Med"},{"key":"2026030623364650100_bib31","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1084\/jem.172.1.391","volume":"172","year":"1990","journal-title":"J Exp Med"},{"key":"2026030623364650100_bib32","doi-asserted-by":"crossref","first-page":"1843","DOI":"10.4049\/jimmunol.146.6.1843","volume":"146","year":"1991","journal-title":"J Immunol"},{"key":"2026030623364650100_bib33","doi-asserted-by":"crossref","first-page":"18432","DOI":"10.1074\/jbc.M906304199","volume":"275","year":"2000","journal-title":"J Biol Chem"},{"key":"2026030623364650100_bib34","first-page":"311","volume":"7","year":"2006","journal-title":"Nat Immunol"},{"key":"2026030623364650100_bib35","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/S1074-7613(03)00171-7","volume":"19","year":"2003","journal-title":"Immunity"},{"key":"2026030623364650100_bib36","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/S0300-483X(99)00182-1","volume":"143","year":"2000","journal-title":"Toxicology"},{"key":"2026030623364650100_bib37","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/BF01404540","volume":"14","year":"1992","journal-title":"J Tissue Cult Methods"},{"key":"2026030623364650100_bib38","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.1097\/01.jom.0000184884.85325.36","volume":"47","year":"2005","journal-title":"J Occup Environ Med"}],"container-title":["American Journal of Respiratory Cell and Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/36\/2\/191\/67088949\/ajrcmb_36_2_191.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/36\/2\/191\/67088949\/ajrcmb_36_2_191.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T04:45:26Z","timestamp":1772858726000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article\/36\/2\/191\/8493624"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,2,1]]},"references-count":38,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2007,2,1]]},"published-print":{"date-parts":[[2007,2,1]]}},"URL":"https:\/\/doi.org\/10.1165\/rcmb.2006-0021tr","relation":{},"ISSN":["1044-1549","1535-4989"],"issn-type":[{"value":"1044-1549","type":"print"},{"value":"1535-4989","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2007,2]]},"published":{"date-parts":[[2007,2,1]]}}}