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However, the precise source of exhaled NO has yet to be identified, and the interpretation is further hampered by significant inter-subject variation. Using fully differentiated normal human bronchial epithelial (NHBE) cells, we sought to determine (1) the rate of NO release (flux, pl\u00b7s\u22121.cm\u22122) into the gas; (2) the effect of IL-13, a prominent mediator of allergic inflammation, on NO release; and (3) inter-subject\/donor variability in NO release. NHBE cells from three different donors were cultured at an air\u2013liquid interface and stimulated with different concentrations of IL-13 (0, 1, and 10 ng\/ml) for 48 h. Gas phase NO concentrations in the headspace over the cells were measured using a chemiluminescence analyzer. The basal NO flux from the three donors (0.05 \u00b1 0.03) is similar in magnitude to that estimated from exhaled NO concentrations, and was significantly increased by IL-13 in a donor-specific fashion. The increase in NO release was strongly correlated with inducible nitric oxide synthase (iNOS) gene and protein expression. There was a trend toward enhanced production of nitrate relative to nitrite as an end product of NO metabolism in IL-13\u2013stimulated cells. NO release from airway epithelial cells can be directly measured. The rate of release in response to IL-13 is strongly dependent on the individual donor, but is primarily due to the expression of iNOS.<\/jats:p>","DOI":"10.1165\/rcmb.2006-0419oc","type":"journal-article","created":{"date-parts":[[2007,3,8]],"date-time":"2007-03-08T21:14:47Z","timestamp":1173388487000},"page":"97-104","source":"Crossref","is-referenced-by-count":102,"title":["Measurement of IL-13\u2013Induced iNOS-Derived Gas Phase Nitric Oxide in Human Bronchial Epithelial Cells"],"prefix":"10.1093","volume":"37","author":[{"given":"Vinod","family":"Suresh","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, and Department of Chemical Engineering and Materials Science, University of California Irvine, Irvine, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justin D.","family":"Mih","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, and Department of Chemical Engineering and Materials Science, 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