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Pulmonary manifestations of the disease include the production of thickened mucus and symptoms of asthma, such as cough and wheezing. A possible alteration in airway smooth muscle (ASM) cell function of patients with CF has not been investigated. The aim of this study was to determine whether the (CFTR) channel is present and affects function of human ASM cells. Cell cultures were obtained from the main or lobar bronchi of patients with and without CF, and the presence of the CFTR channel detected by immunofluorescence. Cytosolic Ca2+ was measured using Fura-2 and dual-wavelength microfluorimetry. The results show that CFTR is expressed in airway bronchial tissue and in cultured ASM cells. Peak Ca2+ release in response to histamine was significantly decreased in CF cells compared with non-CF ASM cells (357 \u00b1 53 nM versus 558 \u00b1 20 nM; P &amp;lt; 0.001). The CFTR pharmacological blockers, glibenclamide and N-phenyl anthranilic acid, significantly reduced histamine-induced Ca2+ release in non-CF cells, and similar results were obtained when CFTR expression was varied using antisense oligonucleotides. In conclusion, these data show that the CFTR channel is present in ASM cells, and that it modulates the release of Ca2+ in response to contractile agents. In patients with CF, a dysfunctional CFTR channel could contribute to the asthma diathesis and gastrointestinal problems experienced by these patients.<\/jats:p>","DOI":"10.1165\/rcmb.2006-0444oc","type":"journal-article","created":{"date-parts":[[2008,8,28]],"date-time":"2008-08-28T21:51:59Z","timestamp":1219960319000},"page":"217-222","source":"Crossref","is-referenced-by-count":54,"title":["Role of the Cystic Fibrosis Transmembrane Conductance Channel in Human Airway Smooth Muscle"],"prefix":"10.1093","volume":"40","author":[{"given":"Marie-Claire","family":"Michoud","sequence":"first","affiliation":[{"name":"Seymour Heisler Laboratory of the Montreal Chest Institute and Meakins-Christie Laboratories, McGill University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renaud","family":"Robert","sequence":"additional","affiliation":[{"name":"Physiology Department, McGill University, Montreal, Quebec, Canada; 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