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TGF-\u03b2, phorbol myristate acetate, and the translation inhibitor cycloheximide induced PAI-1 mRNA and slowed its degradation, suggesting that PAI-1 mRNA could be regulated by interaction of a PAI-1 binding protein (PAI-1 mRNABp) with PAI-1 mRNA. We found that an approximately 60 kD cytoplasmic PAI-1 mRNABp is detectable in cytoplasmic extracts of MeT5A human pleural mesothelial and malignant mesothelioma cells. The PAI-1 mRNABp specifically binds to a 33-nt sequence in the 3\u2032 untranslated region of PAI-1 mRNA. Insertion of this 33-nt sequence destabilizes otherwise stable \u03b2-globin mRNA, indicating that the binding sequence accelerates decay of endogenous PAI-1 mRNA. Competitive inhibition by overexpression of the 33-nt binding sequence in MeT5A cells reduced PAI-1 mRNA decay and increased PAI-1 protein and mRNA expression, indicating that the PAI-1 mRNABp destabilizes PAI-1 mRNA by its interaction with the endogenous 33-nt binding sequence. Incubation of Met5A cells with TGF-\u03b2 attenuated the interaction of the PAI-1 mRNABp with the 33-nt sequence. By conventional and affinity purification, we isolated the PAI-1 mRNABp and confirmed its identity as 6-phospho-d-gluconate-NADP oxidoreductase, which specifically interacts with the full-length and the 33-nt sequence of the PAI-1 mRNA 3\u2032 untranslated region. This newly recognized pathway could influence expression of PAI-1 by mesothelial or mesothelioma cells at the level of mRNA stability in the context of pleural inflammation or malignancy.<\/jats:p>","DOI":"10.1165\/rcmb.2009-0046oc","type":"journal-article","created":{"date-parts":[[2009,10,23]],"date-time":"2009-10-23T21:29:57Z","timestamp":1256333397000},"page":"358-367","source":"Crossref","is-referenced-by-count":13,"title":["Post-Transcriptional Regulation of Plasminogen Activator Inhibitor Type\u20131 Expression in Human Pleural Mesothelial Cells"],"prefix":"10.1093","volume":"43","author":[{"given":"Sreerama","family":"Shetty","sequence":"first","affiliation":[{"name":"Texas Lung Injury Institute, The University of Texas Health Science Center at Tyler, Tyler, Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thirunavukkarasu","family":"Velusamy","sequence":"additional","affiliation":[{"name":"Texas Lung Injury Institute, The University of Texas Health 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