{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T00:04:07Z","timestamp":1772928247991,"version":"3.50.1"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2010,8,1]],"date-time":"2010-08-01T00:00:00Z","timestamp":1280620800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Chronic obstructive pulmonary disease (COPD) involves disease of small airways with an increase in airway smooth muscle sensitivity to spasmogens and with structural changes described as airway remodeling. We investigated the effect of tobacco smoke (TS) exposure on the structure and function of small airways in rats and the role of IL-13 in this response. Precision-cut lung slices (230\u2013280 \u03bcm) were prepared from male Sprague-Dawley rats after acute (3 d) or chronic (8 or 16 wk) daily exposure to TS or air. Carbachol (CCh) and 5-hydroxytryptamine (5HT) concentration responses were performed on airways (50\u2013400 \u03bcm diameter). The effect of IL-13 in vitro on small airway sensitivity to CCh and 5HT was also determined. Acute exposure to TS did not affect the sensitivity of the intrapulmonary airways to either spasmogen. After 8 weeks of TS exposure, airway hyperresponsiveness (AHR) to CCh was evident (log EC50 CCh: air = 0.22 \u03bcM; TS = \u22120.12 \u03bcM; P = 0.019); AHR to 5HT was also observed after the 16-week exposure to TS (air = \u22120.85 \u03bcM; TS = \u22121.06 \u03bcM; P = 0.038). Chronic TS exposure increased airway wall SMA content, which correlated with increased expression of IL-13 and transforming growth factor (TGF)-\u03b21 in the lung tissues. In vitro incubation with IL-13, but not TGF-\u03b21, induced changes in small airway sensitivity to CCh and 5HT. Chronic TS exposure induces increased responsiveness in intrapulmonary airways of rats that may be mediated in part by an increase in IL-13.<\/jats:p>","DOI":"10.1165\/rcmb.2009-0117oc","type":"journal-article","created":{"date-parts":[[2009,9,25]],"date-time":"2009-09-25T22:04:22Z","timestamp":1253916262000},"page":"220-226","source":"Crossref","is-referenced-by-count":33,"title":["Involvement of IL-13 in Tobacco Smoke\u2013Induced Changes in the Structure and Function of Rat Intrapulmonary Airways"],"prefix":"10.1093","volume":"43","author":[{"given":"Philip R.","family":"Cooper","sequence":"first","affiliation":[{"name":"Section of Airway Disease, National Heart and Lung Institute, Dovehouse Street, London; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher T.","family":"Poll","sequence":"additional","affiliation":[{"name":"Respiratory Disease Area, Novartis Institutes for Biomedical Research, Horsham, West Sussex, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter J.","family":"Barnes","sequence":"additional","affiliation":[{"name":"Section of Airway Disease, National Heart and Lung Institute, Dovehouse Street, London; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. Graham","family":"Sturton","sequence":"additional","affiliation":[{"name":"Section of Airway Disease, National Heart and Lung Institute, Dovehouse Street, London; and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2010,8,1]]},"reference":[{"key":"2026030623240450000_bib1","doi-asserted-by":"crossref","first-page":"2645","DOI":"10.1056\/NEJMoa032158","volume":"350","year":"2004","journal-title":"N Engl J Med"},{"key":"2026030623240450000_bib2","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1164\/rccm.200801-059OC","volume":"178","year":"2008","journal-title":"Am J Respir Crit Care Med"},{"key":"2026030623240450000_bib3","doi-asserted-by":"crossref","first-page":"2343","DOI":"10.1152\/jappl.1992.72.6.2343","volume":"72","year":"1992","journal-title":"J Appl 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