{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T03:28:51Z","timestamp":1787887731861,"version":"build-2784847793"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2011,10,1]],"date-time":"2011-10-01T00:00:00Z","timestamp":1317427200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>A subset of workers in swine confinement facilities develops chronic respiratory disease. An aqueous extract of dust from these facilities (hogbarn dust extract [HDE]) induces IL-6 and IL-8 release and several other responses in isolated airway epithelial cells. The cell membrane receptors by which HDE initiates these responses have not been identified. Because several other inhaled agents induce airway epithelial cell responses through epidermal growth factor receptor (EGFR) activation, we hypothesized that HDE would activate EGFRs and that EGFRs would be required for some of the responses to HDE. Exposure of Beas-2B cells to HDE caused EGFR phosphorylation and downstream ERK activation, and both responses were blocked by the EGFR-selective kinase inhibitor AG1478. AG1478 and EGFR-neutralizing antibody reduced HDE-stimulated IL-6 and IL-8 release by about half. Similar EGFR phosphorylation and requirement of EGFRs for maximal IL-6 and IL-8 release were found with primary isolates of human bronchial epithelial cells. Because HDE-stimulated IL-6 and IL-8 release involve the Ca2+\u2013dependent protein kinase C\u03b1, we hypothesized that HDE would induce intracellular Ca2+ mobilization. HDE exposure induced intracellular Ca2+ mobilization in Beas-2B cells and in primary cell isolates, but this response was neither mimicked by EGF nor inhibited by AG1478. Thus, HDE activates EGFRs and their downstream signaling, and EGFR activation is required for some but not all airway epithelial cell responses to HDE.<\/jats:p>","DOI":"10.1165\/rcmb.2010-0419oc","type":"journal-article","created":{"date-parts":[[2011,3,25]],"date-time":"2011-03-25T22:20:45Z","timestamp":1301091645000},"page":"882-888","source":"Crossref","is-referenced-by-count":12,"title":["Airway Epithelial Epidermal Growth Factor Receptor Mediates Hogbarn Dust\u2013Induced Cytokine Release but Not Ca2+ Response"],"prefix":"10.1093","volume":"45","author":[{"given":"Puttappa R.","family":"Dodmane","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy A.","family":"Schulte","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, Omaha"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Art J.","family":"Heires","sequence":"additional","affiliation":[{"name":"Pulmonary, Critical Care, Sleep and Allergy Section, Department of Internal Medicine, University of Nebraska Medical Center, Omaha"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamid","family":"Band","sequence":"additional","affiliation":[{"name":"Eppley Institute for Research in Cancer and Allied Diseases and Departments of Biochemistry & Molecular Biology; Genetics, Cell Biology & Anatomy; Pathology & Microbiology; and Pharmacology & Experimental Neuroscience; University of Nebraska Medical Center, Omaha; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debra J.","family":"Romberger","sequence":"additional","affiliation":[{"name":"Pulmonary, Critical Care, Sleep and Allergy Section, Department of Internal Medicine, University of Nebraska Medical Center, Omaha"},{"name":"Veterans Administration 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