{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T10:25:05Z","timestamp":1771496705896,"version":"3.50.1"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2012,9,1]],"date-time":"2012-09-01T00:00:00Z","timestamp":1346457600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Insulin-like growth factor (IGF)-1 is increased in different models of acute lung injury, and is an important determinant of survival and proliferation in many cells. We previously demonstrated that treatment of mice with IGF-1 receptor\u2013blocking antibody (A12) improved early survival in bleomycin-induced lung injury. We have now examined whether administration of A12 improved markers of lung injury in hyperoxia model of lung injury. C57BL\/6 mice underwent intraperitoneal administration of A12 or control antibody (keyhole limpet hemocyanin [KLH]), then were exposed to 95% hyperoxia for 88\u201390 hours. Mice were killed and bronchoalveolar lavage (BAL) and lung tissue were obtained for analysis. Hyperoxia caused a significant increase in IGF levels in BAL and lung lysates. Peripheral blood neutrophils expressed IGF-1R at baseline and after hyperoxia. BAL neutrophils from hyperoxia-treated mice and patients with acute lung injury also expressed cell surface IGF-1R. A12-treated mice had significantly decreased polymorphonuclear cell (PMN) count in BAL compared with KLH control mice (P = 0.02). BAL from A12-treated mice demonstrated decreased PMN chemotactic activity compared with BAL from KLH-treated mice. Pretreatment of PMNs with A12 decreased their chemotactic response to BAL from hyperoxia-exposed mice. Furthermore, IGF-1 induced a dose-dependent chemotaxis of PMNs. There were no differences in other chemotactic cytokines in BAL, including CXCL1 and CXCL2. In summary, IGF blockade decreased PMN recruitment to the alveolar space in a mouse model of hyperoxia. Furthermore, the decrease in BAL PMNs was at least partially due to a direct effect of A12 on PMN chemotaxis.<\/jats:p>","DOI":"10.1165\/rcmb.2012-0085oc","type":"journal-article","created":{"date-parts":[[2012,4,5]],"date-time":"2012-04-05T22:02:17Z","timestamp":1333663337000},"page":"372-378","source":"Crossref","is-referenced-by-count":14,"title":["Effect of Insulin-Like Growth Factor Blockade on Hyperoxia-Induced Lung Injury"],"prefix":"10.1093","volume":"47","author":[{"given":"Tae-Hyung","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Medicine, Division of Pulmonary and Critical Care Medicine, Hanyang University College of Medicine, Seoul, Korea; and"},{"name":"Center for Lung Biology, Department of Pulmonary and Critical Care Medicine, University of Washington School of Medicine, Seattle, Washington"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Hua","family":"Chow","sequence":"additional","affiliation":[{"name":"Center for Lung 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