{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T21:48:25Z","timestamp":1792705705342,"version":"4.5.0"},"reference-count":17,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Many human lung diseases, such as asthma, chronic obstructive pulmonary disease, bronchiolitis obliterans, and cystic fibrosis, are characterized by changes in the cellular composition and architecture of the airway epithelium. Intravital fluorescence microscopy has emerged as a powerful approach in mechanistic studies of diseases, but it has been difficult to apply this tool for in vivo respiratory cell biology in animals in a minimally invasive manner. Here, we describe a novel miniature side-view confocal probe capable of visualizing the epithelium in the mouse trachea in vivo at a single-cell resolution. We performed serial real-time endotracheal fluorescence microscopy in live transgenic reporter mice to view the three major cell types of the large airways, namely, basal cells, Clara cells, and ciliated cells. As a proof-of-concept demonstration, we monitored the regeneration of Clara cells over 18 days after a sulfur dioxide injury. Our results show that in vivo tracheal microscopy offers a new approach in the study of altered, regenerating, or metaplastic airways in animal models of lung diseases.<\/jats:p>","DOI":"10.1165\/rcmb.2012-0164oc","type":"journal-article","created":{"date-parts":[[2012,9,15]],"date-time":"2012-09-15T06:44:03Z","timestamp":1347691443000},"page":"864-868","source":"Crossref","is-referenced-by-count":29,"title":["<i>In Vivo<\/i>\n                    Imaging of Tracheal Epithelial Cells in Mice during Airway Regeneration"],"prefix":"10.1093","volume":"47","author":[{"given":"Jun Ki","family":"Kim","sequence":"first","affiliation":[{"name":"Harvard Medical School and Wellman Center for Photomedicine, and"},{"name":"WCU Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology, Daejeon, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir","family":"Vinarsky","sequence":"additional","affiliation":[{"name":"Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Wain","sequence":"additional","affiliation":[{"name":"Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Zhao","sequence":"additional","affiliation":[{"name":"Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keehoon","family":"Jung","sequence":"additional","affiliation":[{"name":"Harvard Medical School and Wellman Center for Photomedicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinwoo","family":"Choi","sequence":"additional","affiliation":[{"name":"Harvard Medical School and Wellman Center for Photomedicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Lam","sequence":"additional","affiliation":[{"name":"Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Pardo-Saganta","sequence":"additional","affiliation":[{"name":"Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Breton","sequence":"additional","affiliation":[{"name":"Center for Systems Biology, Program in Membrane Biology\/Nephrology Division, Massachusetts General Hospital, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jayaraj","family":"Rajagopal","sequence":"additional","affiliation":[{"name":"Center for Regenerative Medicine, Massachusetts General Hospital, Boston, Massachusetts"},{"name":"Harvard Stem Cell Institute, Holyoke Center, Cambridge, Massachusetts; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seok Hyun","family":"Yun","sequence":"additional","affiliation":[{"name":"Harvard Medical School and Wellman Center for Photomedicine, and"},{"name":"WCU Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology, Daejeon, Korea"},{"name":"Harvard\u2013Massachusetts Institute of Technology Health Sciences and Technology, Cambridge, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2012,12,1]]},"reference":[{"key":"2026021904163353400_bib1","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1242\/dmm.006031","article-title":"Airway basal stem cells: a perspective on their roles in epithelial homeostasis and remodeling","volume":"3","author":"Rock","year":"2010","journal-title":"Dis Model 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