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In this study, using virus deletion mutants and silencing for nucleotide-binding oligomerization domain\u2013like receptors (NLRs), we show that the rhinovirus ion channel protein 2B triggers NLRP3 and NLRC5 inflammasome activation and IL-1\u03b2 secretion in bronchial cells. 2B protein targets the endoplasmic reticulum and Golgi and induces Ca2+ reduction in these organelles, thereby disturbing the intracellular calcium homeostasis. NLRP3 and NLRC5 act in a cooperative manner during the inflammasome assembly by sensing intracellular Ca2+ fluxes and trigger IL-1\u03b2 secretion. These results reveal for the first time that human rhinovirus infection in primary bronchial cells triggers inflammasome activation.<\/jats:p>","DOI":"10.1165\/rcmb.2013-0032oc","type":"journal-article","created":{"date-parts":[[2013,7,1]],"date-time":"2013-07-01T15:54:29Z","timestamp":1372694069000},"page":"923-934","source":"Crossref","is-referenced-by-count":130,"title":["Rhinovirus-Induced Calcium Flux Triggers NLRP3 and NLRC5 Activation in Bronchial Cells"],"prefix":"10.1093","volume":"49","author":[{"given":"Kathy","family":"Triantafilou","sequence":"first","affiliation":[{"name":"Cardiff University, Institute of Infection and Immunity, Department of Child Health, School of Medicine, University Hospital of Wales, Heath Park, Cardiff, Wales; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satwik","family":"Kar","sequence":"additional","affiliation":[{"name":"Cardiff University, Institute of Infection and Immunity, Department of Child Health, School of Medicine, University Hospital of Wales, Heath Park, Cardiff, Wales; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank J. 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