{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T03:16:21Z","timestamp":1791256581072,"version":"4.1.0"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2014,2,1]],"date-time":"2014-02-01T00:00:00Z","timestamp":1391212800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Local derangements of fibrin turnover and plasminogen activator inhibitor (PAI)-1 have been implicated in the pathogenesis of pleural injury. However, their role in the control of pleural organization has been unclear. We found that a C57Bl\/6j mouse model of carbon black\/bleomycin (CBB) injury demonstrates pleural organization resulting in pleural rind formation (14 d). In transgenic mice overexpressing human PAI-1, intrapleural fibrin deposition was increased, but visceral pleural thickness, lung volumes, and compliance were comparable to wild type. CBB injury in PAI-1\u2212\/\u2212 mice significantly increased visceral pleural thickness (P &amp;lt; 0.001), elastance (P &amp;lt; 0.05), and total lung resistance (P &amp;lt; 0.05), while decreasing lung compliance (P &amp;lt; 0.01) and lung volumes (P &amp;lt; 0.05). Collagen, \u03b1-smooth muscle actin, and tissue factor were increased in the thickened visceral pleura of PAI-1\u2212\/\u2212 mice. Colocalization of \u03b1-smooth muscle actin and calretinin within pleural mesothelial cells was increased in CBB-injured PAI-1\u2212\/\u2212 mice. Thrombin, factor Xa, plasmin, and urokinase induced mesothelial\u2013mesenchymal transition, tissue factor expression, and activity in primary human pleural mesothelial cells. In PAI-1\u2212\/\u2212 mice, D-dimer and thrombin\u2013antithrombin complex concentrations were increased in pleural lavage fluids. The results demonstrate that PAI-1 regulates CBB-induced pleural injury severity via unrestricted fibrinolysis and cross-talk with coagulation proteases. Whereas overexpression of PAI-1 augments intrapleural fibrin deposition, PAI-1 deficiency promotes profibrogenic alterations of the mesothelium that exacerbate pleural organization and lung restriction.<\/jats:p>","DOI":"10.1165\/rcmb.2013-0300oc","type":"journal-article","created":{"date-parts":[[2013,9,11]],"date-time":"2013-09-11T17:17:07Z","timestamp":1378919827000},"page":"316-327","source":"Crossref","is-referenced-by-count":43,"title":["Plasminogen Activator Inhibitor-1 Deficiency Augments Visceral Mesothelial Organization, Intrapleural Coagulation, and Lung Restriction in Mice with Carbon Black\/Bleomycin\u2013Induced Pleural Injury"],"prefix":"10.1093","volume":"50","author":[{"given":"Torry A.","family":"Tucker","sequence":"first","affiliation":[{"name":"The Texas Lung Injury Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann","family":"Jeffers","sequence":"additional","affiliation":[{"name":"The Texas Lung Injury Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexia","family":"Alvarez","sequence":"additional","affiliation":[{"name":"The Texas Lung Injury Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuzi","family":"Owens","sequence":"additional","affiliation":[{"name":"The Texas Lung Injury Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen","family":"Koenig","sequence":"additional","affiliation":[{"name":"The Texas Lung Injury Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brandon","family":"Quaid","sequence":"additional","affiliation":[{"name":"The Texas Lung Injury Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrey A.","family":"Komissarov","sequence":"additional","affiliation":[{"name":"The Texas Lung Injury Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Galina","family":"Florova","sequence":"additional","affiliation":[{"name":"The Texas Lung Injury Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hema","family":"Kothari","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Biology, University of Texas Health Science Center at Tyler, Tyler, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Usha","family":"Pendurthi","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Biology, University of Texas Health Science Center at Tyler, Tyler, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. 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