{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T02:51:16Z","timestamp":1792291876960,"version":"4.4.0"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2015,7,1]],"date-time":"2015-07-01T00:00:00Z","timestamp":1435708800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>We have developed a transgenic mouse expressing enhanced green fluorescent protein (EGFP) in virtually all type II (TII) alveolar epithelial cells. The CBG mouse (SPC-BAC-EGFP) contains a bacterial artificial chromosome modified to express EGFP within the mouse surfactant protein (SP)-C gene 3\u2032 untranslated region. EGFP mRNA expression is limited to the lung. EGFP fluorescence is both limited to and exhibited by all cells expressing pro\u2013SP-C; fluorescence is uniform throughout all lobes of the lung and does not change as mice age. EGFP+ cells also express SP-B but do not express podoplanin, a type I (TI) cell marker. CBG mice show no evidence of lung disease with aging. In 3 hours, TII cells can be isolated in &amp;gt;99% purity from CBG mice by FACS; the yield of 3.7\u2009\u00b1\u20090.6\u2009\u00d7\u2009106 cells represents approximately 25 to 60% of the TII cells in the lung. By FACS analysis, approximately 0.9% of TII cells are in mitosis in uninjured lungs; after bleomycin injury, 4.1% are in mitosis. Because EGFP fluorescence can be detected for &amp;gt;14 days in culture, at a time that SP-C mRNA expression is essentially nil, this line may be useful for tracking TII cells in culture and in vivo. When CBG mice are crossed to transgenic mice expressing rat podoplanin, TI and TII cells can be easily simultaneously identified and isolated. When bred to other strains of mice, EGFP expression can be used to identify TII cells without the need for immunostaining for SP-C. These mice should be useful in models of mouse pulmonary disease and in studies of TII cell biology, biochemistry, and genetics.<\/jats:p>","DOI":"10.1165\/rcmb.2014-0348ma","type":"journal-article","created":{"date-parts":[[2015,2,18]],"date-time":"2015-02-18T16:48:56Z","timestamp":1424278136000},"page":"14-21","source":"Crossref","is-referenced-by-count":22,"title":["High-Efficiency Type II Cell\u2013Enhanced Green Fluorescent Protein Expression Facilitates Cellular Identification, Tracking, and Isolation"],"prefix":"10.1093","volume":"53","author":[{"given":"Jeff N.","family":"Vanderbilt","sequence":"first","affiliation":[{"name":"Cardiovascular Research Institute and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert F.","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Institute and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lennell","family":"Allen","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Institute and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"AnneMarie","family":"Gillespie","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Institute and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Leaffer","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Institute and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willow B.","family":"Dean","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Institute and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheryl","family":"Chapin","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leland G.","family":"Dobbs","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Institute and"},{"name":"Departments of Pediatrics and"},{"name":"Medicine, University of California, San Francisco, San Francisco, California"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2015,7,1]]},"reference":[{"key":"2026021122332239500_bib1","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1165\/ajrcmb.10.6.8003339","article-title":"Cell number and distribution in human and rat airways","volume":"10","author":"Mercer","year":"1994","journal-title":"Am J Respir Cell Mol Biol"},{"key":"2026021122332239500_bib2","doi-asserted-by":"crossref","first-page":"594","DOI":"10.1165\/ajrcmb\/6.6.594","article-title":"Surfactant protein C is expressed in alveolar type II cells but not in C lara cells of rat lung","volume":"6","author":"Kalina","year":"1992","journal-title":"Am J Respir Cell Mol Biol"},{"key":"2026021122332239500_bib3","doi-asserted-by":"crossref","first-page":"22147","DOI":"10.1074\/jbc.M201812200","article-title":"Identification of LBM180, a lamellar body limiting membrane protein of alveolar type II cells, as the ABC transporter protein ABCA3","volume":"277","author":"Mulugeta","year":"2002","journal-title":"J Biol Chem"},{"key":"2026021122332239500_bib4","first-page":"141","article-title":"An improved method for isolating type II cells in high yield and purity","volume":"134","author":"Dobbs","year":"1986","journal-title":"Am Rev Respir Dis"},{"key":"2026021122332239500_bib5","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/0167-4889(88)90173-5","article-title":"Monoclonal antibodies specific to apical surfaces of rat alveolar type I cells bind to surfaces of cultured, but not freshly isolated, type II cells","volume":"970","author":"Dobbs","year":"1988","journal-title":"Biochim Biophys Acta"},{"key":"2026021122332239500_bib6","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1165\/ajrcmb.19.4.3121","article-title":"Characterization of the gene and promoter for RTI40, a differentiation marker of type I alveolar epithelial cells","volume":"19","author":"Vanderbilt","year":"1998","journal-title":"Am J Respir Cell Mol Biol"},{"key":"2026021122332239500_bib7","doi-asserted-by":"crossref","first-page":"C1549","DOI":"10.1152\/ajpcell.1997.273.5.C1549","article-title":"Aquaporins in complex tissues: II. 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