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C57\/BL6 mice were exposed to ozone or filtered air over 6 weeks. Sodium hydrogen sulfide (NaHS), an H2S donor, was administered to the mice either before ozone exposure (preventive effect) or after completion of 6 weeks of ozone exposure (therapeutic effect). The ozone-exposed mice developed emphysema, measured by micro\u2013computed tomography and histology, airflow limitation, measured by the forced maneuver system, and increased lung inflammation with augmented IL-1\u03b2, IL-18, and matrix metalloproteinase-9 (MMP-9) gene expression. Ozone-induced changes were associated with increased Nod-like receptor pyrin domain containing 3 (NLRP3)\u2013caspase-1 activation and p38 mitogen-activated protein kinase phosphorylation and decreased Akt phosphorylation. NaHS both prevented and reversed lung inflammation and emphysematous changes in alveolar space. In contrast, NaHS prevented, but did not reverse, ozone-induced airflow limitation and bronchial structural remodeling. In conclusion, NaHS administration prevented and partially reversed ozone-induced features of lung inflammation and emphysema via regulation of the NLRP3\u2013caspase-1, p38 mitogen-activated protein kinase, and Akt pathways.<\/jats:p>","DOI":"10.1165\/rcmb.2015-0014oc","type":"journal-article","created":{"date-parts":[[2016,1,5]],"date-time":"2016-01-05T18:06:28Z","timestamp":1452017188000},"page":"72-81","source":"Crossref","is-referenced-by-count":33,"title":["Hydrogen Sulfide Prevents and Partially Reverses Ozone-Induced Features of Lung Inflammation and Emphysema in Mice"],"prefix":"10.1093","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0646-6560","authenticated-orcid":false,"given":"Feng","family":"Li","sequence":"first","affiliation":[{"name":"Department of Respiratory Medicine and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine 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