{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T10:57:08Z","timestamp":1791889028609,"version":"4.3.4"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T00:00:00Z","timestamp":1548979200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>IFN-\u03bb and IL-22, cytokines that share the coreceptor IL-10RB, are both induced over the course of Klebsiella pneumoniae ST258 (KP35) pneumonia. IL-22 is known to protect mucosal barriers, whereas the effects of IFN-\u03bb on the mucosa are not established. We postulated that IFN-\u03bb plays a role in regulating the airway epithelial barrier to facilitate cellular trafficking to the site of infection. In response to IFN-\u03bb, the transmigration of neutrophils across a polarized monolayer of airway epithelial cells was increased, consistent with diminished epithelial integrity. KP35 infection increased epithelial permeability, and pretreatment with IFN-\u03bb amplified this effect and facilitated bacterial transmigration. These effects of IFN-\u03bb were confirmed in vivo, in that mice lacking the receptor for IFN-\u03bb (Ifnlr1 \u00a0\u2212\/\u2212) were protected from bacteremia in a murine model of KP35 pneumonia. Conversely, the integrity of the epithelial barrier was protected by IL-22, with subsequent impairment of neutrophil and bacterial transmigration in vitro. Maximal expression of IL-22 in vivo was observed later in the course of infection than IFN-\u03bb production, with high levels of IL-22 produced by recruited immune cells at 48 hours, consistent with a role in epithelial barrier recovery. The divergent and opposing expression of these two related cytokines suggests a regulated interaction in the host response to KP35 infection. A major physiological effect of IFN-\u03bb signaling is a decrease in epithelial barrier integrity, which facilitates immune cell recruitment but also enables K. pneumoniae invasion.<\/jats:p>","DOI":"10.1165\/rcmb.2018-0021oc","type":"journal-article","created":{"date-parts":[[2018,9,5]],"date-time":"2018-09-05T11:54:32Z","timestamp":1536148472000},"page":"158-166","source":"Crossref","is-referenced-by-count":53,"title":["The Effects of IFN-\u03bb on Epithelial Barrier Function Contribute to\n                    <i>Klebsiella pneumoniae<\/i>\n                    ST258 Pneumonia"],"prefix":"10.1093","volume":"60","author":[{"given":"Danielle","family":"Ahn","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Wickersham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Riquelme","sequence":"additional","affiliation":[{"name":"Department of 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