{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T13:18:26Z","timestamp":1770815906877,"version":"3.50.1"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2019,8,1]],"date-time":"2019-08-01T00:00:00Z","timestamp":1564617600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Airway smooth muscle cells require coordinated protrusion and focal adhesion dynamics to migrate properly. However, the signaling cascades that connect these two processes remain incompletely understood. Glia maturation factor (GMF)-\u03b3 has been implicated in inducing actin debranching and inhibiting nucleation. In this study, we discovered that GMF\u03b3 phosphorylation at Y104 regulates human airway smooth muscle cell migration. Using high-resolution microscopy coupled with three-dimensional object\u2013based quantitative image analysis software, Imaris 9.2.0, phosphomimetic mutant, Y104D-GMF\u03b3, was enriched at nascent adhesions along the leading edge where it recruited activated neural Wiskott-Aldrich syndrome protein (N-WASP; pY256) to promote actin-branch formation, which enhanced lamellipodial dynamics and limited the growth of focal adhesions. Unexpectedly, we found that nonphosphorylated mutant, Y104F-GMF\u03b3, was enriched in growing adhesions where it promoted a linear branch organization and focal adhesion clustering, and recruited zyxin to increase maturation, thus inhibiting lamellipodial dynamics and cell migration. The localization of GMF\u03b3 between the leading edge and focal adhesions was dependent upon myosin activity. Furthermore, c-Abl tyrosine kinase regulated the GMF\u03b3 phosphorylation\u2013dependent processes. Together, these results unveil the importance of GMF\u03b3 phosphorylation in coordinating lamellipodial and focal adhesion dynamics to regulate cell migration.<\/jats:p>","DOI":"10.1165\/rcmb.2018-0352oc","type":"journal-article","created":{"date-parts":[[2019,2,27]],"date-time":"2019-02-27T19:21:40Z","timestamp":1551295300000},"page":"219-231","source":"Crossref","is-referenced-by-count":16,"title":["Phosphorylation of GMF\u03b3 by c-Abl Coordinates Lamellipodial and Focal Adhesion Dynamics to Regulate Airway Smooth Muscle Cell Migration"],"prefix":"10.1093","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2791-3534","authenticated-orcid":false,"given":"Brennan D.","family":"Gerlach","sequence":"first","affiliation":[{"name":"Department of Molecular Cellular Physiology, Albany Medical College, Albany, New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kate","family":"Tubbesing","sequence":"additional","affiliation":[{"name":"Department of 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