{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T22:22:23Z","timestamp":1787955743275,"version":"build-2784847793"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T00:00:00Z","timestamp":1770768000000},"content-version":"vor","delay-in-days":2417,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Glucocorticoids, commonly used asthma controller medications, decrease symptoms in most patients, but some remain symptomatic despite high-dose treatment. The physiological basis underlying the glucocorticoid response, especially in asthma patients with severe, refractory disease, is not fully understood. We sought to identify differences between the transcriptomic response of airway smooth muscle (ASM) cells derived from donors with fatal asthma and donors without asthma to glucocorticoid exposure and to compare ASM-specific changes with those observed in other cell types. In cells derived from nine donors with fatal asthma and eight donors without asthma, RNA sequencing was used to measure ASM transcriptome changes after exposure to budesonide (100 nM 24 h) or control vehicle (DMSO). Differential expression results were obtained for this dataset, as well as 13 publicly available glucocorticoid-response transcriptomic datasets corresponding to seven cell types. Specific genes were differentially expressed in response to glucocorticoid exposure (7,835 and 6,957 in ASM cells derived from donors with fatal asthma and donors without asthma, respectively; adjusted P value\u2009&amp;lt;\u20090.05). Transcriptomic changes in response to glucocorticoid exposure were similar in ASM derived from donors with fatal asthma and donors without asthma, with enriched ontological pathways that included cytokine- and chemokine-related categories. A comparison of glucocorticoid-induced changes in the nonasthma ASM transcriptome with those observed in six other cell types showed that ASM has a distinct glucocorticoid-response signature that is also present in ASM cells from donors with fatal asthma.<\/jats:p>","DOI":"10.1165\/rcmb.2018-0385oc","type":"journal-article","created":{"date-parts":[[2019,1,29]],"date-time":"2019-01-29T15:14:24Z","timestamp":1548774864000},"page":"110-120","source":"Crossref","is-referenced-by-count":31,"title":["Airway Smooth Muscle\u2013Specific Transcriptomic Signatures of Glucocorticoid Exposure"],"prefix":"10.1093","volume":"61","author":[{"given":"Mengyuan","family":"Kan","sequence":"first","affiliation":[{"name":"Department of Biostatistics, Epidemiology and Informatics, University of Pennsylvania, Philadelphia, Pennsylvania; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia","family":"Koziol-White","sequence":"additional","affiliation":[{"name":"Rutgers Institute for Translational Medicine and Science, Rutgers University, New Brunswick, New Jersey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maya","family":"Shumyatcher","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, Epidemiology and Informatics, University of Pennsylvania, Philadelphia, Pennsylvania; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Johnson","sequence":"additional","affiliation":[{"name":"Rutgers Institute for Translational Medicine and Science, Rutgers University, New Brunswick, New Jersey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Jester","sequence":"additional","affiliation":[{"name":"Rutgers Institute for Translational Medicine and Science, Rutgers University, New Brunswick, New Jersey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Reynold A.","family":"Panettieri","sequence":"additional","affiliation":[{"name":"Rutgers Institute for Translational Medicine and Science, Rutgers University, New Brunswick, New Jersey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2868-1333","authenticated-orcid":false,"given":"Blanca E.","family":"Himes","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, Epidemiology and Informatics, University of Pennsylvania, Philadelphia, Pennsylvania; and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,7,1]]},"reference":[{"key":"2026021100375483700_bib1","first-page":"1","article-title":"Trends in asthma prevalence, health care use, and mortality in the United States, 2001\u20132010","volume-title":"NCHS Data Brief","author":"Akinbami","year":"2012"},{"key":"2026021100375483700_bib2","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.1056\/NEJMra0804579","article-title":"Asthma","volume":"360","author":"Fanta","year":"2009","journal-title":"N Engl J Med"},{"key":"2026021100375483700_bib3","doi-asserted-by":"crossref","first-page":"2341","DOI":"10.1164\/ajrccm.162.6.ats9-00","article-title":"Proceedings of the ATS workshop on refractory asthma: current understanding, recommendations, and unanswered questions. 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