{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T22:04:20Z","timestamp":1784153060272,"version":"3.55.0"},"reference-count":68,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["www.atsjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Cystic fibrosis (CF) is caused by loss-of-function mutations in the CFTR (CF transmembrane regulator) gene. Pharmacologic therapies directed at CFTR have been developed but are not effective for mutations that result in little or no mRNA or protein expression. Cell therapy is a potential mutation-agnostic approach to treatment. One strategy is to harvest human bronchial epithelial cells (HBECs) for gene addition or genetic correction, followed by expansion and engraftment. This approach will require cells to grow extensively while retaining their ability to reconstitute CFTR activity. We hypothesized that conditionally reprogrammed cell (CRC) technology, namely growth in the presence of irradiated feeder cells and a Rho kinase inhibitor, would enable expansion while maintaining cell capacity to express functional CFTR. Our goal was to compare expression of the basal cell marker NGFR (nerve growth factor receptor) and three-dimensional bronchosphere colony-forming efficiency (CFE) in early- and later-passage HBECs grown using nonproprietary bronchial epithelial growth medium or the CRC method. Cell number and CFTR activity were determined in a competitive repopulation assay employing chimeric air\u2013liquid interface cultures. HBECs expanded using the CRC method expressed the highest NGFR levels, had the greatest 3D colony-forming efficiency at later passage, generated greater cell numbers in chimeric cultures, and most effectively reconstituted CFTR activity. In our study, the HBEC air\u2013liquid interface model, an informative testing platform proven vital for the development of other CF therapies, illustrated that cells grown by CRC technology or equivalent methods may be useful for cell therapy of CF.<\/jats:p>","DOI":"10.1165\/rcmb.2019-0384oc","type":"journal-article","created":{"date-parts":[[2020,5,21]],"date-time":"2020-05-21T15:17:18Z","timestamp":1590074238000},"page":"374-385","update-policy":"https:\/\/doi.org\/10.1164\/crossmarkpolicies","source":"Crossref","is-referenced-by-count":28,"title":["Assessing Human Airway Epithelial Progenitor Cells for Cystic Fibrosis Cell Therapy"],"prefix":"10.1093","volume":"63","author":[{"given":"Rhianna E.","family":"Lee","sequence":"first","affiliation":[{"name":"Marsico Lung Institute\/Cystic Fibrosis Center and"},{"name":"Department of Cell Biology and Physiology, School of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean M.","family":"Miller","sequence":"additional","affiliation":[{"name":"Marsico Lung Institute\/Cystic Fibrosis Center and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa M.","family":"Mascenik","sequence":"additional","affiliation":[{"name":"Marsico Lung Institute\/Cystic Fibrosis Center and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine A.","family":"Lewis","sequence":"additional","affiliation":[{"name":"Marsico Lung Institute\/Cystic Fibrosis Center and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Dang","sequence":"additional","affiliation":[{"name":"Marsico Lung Institute\/Cystic Fibrosis Center and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zachary H.","family":"Boggs","sequence":"additional","affiliation":[{"name":"Marsico Lung Institute\/Cystic Fibrosis Center and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Tarran","sequence":"additional","affiliation":[{"name":"Marsico Lung Institute\/Cystic Fibrosis Center and"},{"name":"Department of Cell Biology and Physiology, School of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott H.","family":"Randell","sequence":"additional","affiliation":[{"name":"Marsico Lung Institute\/Cystic Fibrosis Center and"},{"name":"Department of Cell Biology and Physiology, School of Medicine, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,9,1]]},"reference":[{"key":"2026021100441584000_bib1","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1038\/nrg3849","article-title":"Cystic fibrosis genetics: from molecular understanding to clinical application","volume":"16","author":"Cutting","year":"2015","journal-title":"Nat Rev Genet"},{"key":"2026021100441584000_bib2","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1016\/j.coph.2017.09.018","article-title":"Transformative therapies for rare CFTR missense alleles","volume":"34","author":"Oliver","year":"2017","journal-title":"Curr Opin Pharmacol"},{"key":"2026021100441584000_bib3","doi-asserted-by":"crossref","first-page":"S52","DOI":"10.1016\/j.jcf.2017.09.005","article-title":"Recent progress in translational cystic fibrosis research using precision medicine strategies","volume":"17","author":"Cholon","year":"2018","journal-title":"J Cyst Fibros"},{"key":"2026021100441584000_bib4","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1089\/jamp.2015.1229","article-title":"The influence of nebulized drugs on nasal ciliary activity","volume":"29","author":"Boon","year":"2016","journal-title":"J Aerosol Med Pulm Drug Deliv"},{"key":"2026021100441584000_bib5","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.jcf.2016.10.001","article-title":"The fate of inhaled antibiotics after deposition in cystic fibrosis: how to get drug to the bug?","volume":"16","author":"Bos","year":"2017","journal-title":"J Cyst Fibros"},{"issue":"12","key":"2026021100441584000_bib6","first-page":"CD001401","article-title":"Chest physiotherapy compared to no chest physiotherapy for cystic fibrosis","author":"Warnock","year":"2015","journal-title":"Cochrane Database Syst Rev"},{"key":"2026021100441584000_bib7","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1097\/MOP.0000000000000627","article-title":"Cystic fibrosis transmembrane conductance regulator modulators: precision medicine in cystic fibrosis","volume":"30","author":"Burgener","year":"2018","journal-title":"Curr Opin Pediatr"},{"key":"2026021100441584000_bib8","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1091\/mbc.e14-04-0935","article-title":"From CFTR biology toward combinatorial pharmacotherapy: expanded classification of cystic fibrosis mutations","volume":"27","author":"Veit","year":"2016","journal-title":"Mol Biol Cell"},{"key":"2026021100441584000_bib9","doi-asserted-by":"crossref","first-page":"1809","DOI":"10.1056\/NEJMoa1908639","article-title":"Elexacaftor-tezacaftor-ivacaftor for cystic fibrosis with a single Phe508del allele","volume":"381","author":"Middleton","year":"2019","journal-title":"N Engl J Med"},{"key":"2026021100441584000_bib10","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.pharmthera.2014.06.005","article-title":"New pharmacological approaches for cystic fibrosis: promises, progress, pitfalls","volume":"145","author":"Bell","year":"2015","journal-title":"Pharmacol Ther"},{"key":"2026021100441584000_bib11","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1016\/S2213-2600(15)00245-3","article-title":"Repeated nebulisation of non-viral CFTR gene therapy in patients with cystic fibrosis: a randomised, double-blind, placebo-controlled, phase 2b trial","volume":"3","author":"Alton","year":"2015","journal-title":"Lancet Respir Med"},{"key":"2026021100441584000_bib12","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1089\/hum.2007.022","article-title":"Repeated aerosolized AAV-CFTR for treatment of cystic fibrosis: a randomized placebo-controlled phase 2B trial","volume":"18","author":"Moss","year":"2007","journal-title":"Hum Gene Ther"},{"key":"2026021100441584000_bib13","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1089\/hum.2016.152","article-title":"Genetic modification of the lung directed toward treatment of human disease","volume":"28","author":"Sondhi","year":"2017","journal-title":"Hum Gene Ther"},{"key":"2026021100441584000_bib14","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1016\/j.jconrel.2016.05.031","article-title":"Barriers to inhaled gene therapy of obstructive lung diseases: a review","volume":"240","author":"Kim","year":"2016","journal-title":"J Control Release"},{"key":"2026021100441584000_bib15","first-page":"493","article-title":"Evading the immune response upon in vivo gene therapy with viral vectors","volume":"11","author":"Sack","year":"2009","journal-title":"Curr Opin Mol Ther"},{"key":"2026021100441584000_bib16","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1002\/stem.2450","article-title":"Concise review: human embryonic stem cells\u2014what have we done? What are we doing? Where are we going?","volume":"35","author":"Ilic","year":"2017","journal-title":"Stem Cells"},{"key":"2026021100441584000_bib17","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1161\/CIRCRESAHA.111.256149","article-title":"Steps toward safe cell therapy using induced pluripotent stem cells","volume":"112","author":"Okano","year":"2013","journal-title":"Circ Res"},{"key":"2026021100441584000_bib18","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1016\/j.stemcr.2014.07.005","article-title":"Generation of alveolar epithelial spheroids via isolated progenitor cells from human pluripotent stem cells","volume":"3","author":"Gotoh","year":"2014","journal-title":"Stem Cell Rep"},{"key":"2026021100441584000_bib19","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.stemcr.2015.11.010","article-title":"Directed induction of functional multi-ciliated cells in proximal airway epithelial spheroids from human pluripotent stem cells","volume":"6","author":"Konishi","year":"2016","journal-title":"Stem Cell Rep"},{"key":"2026021100441584000_bib20","doi-asserted-by":"crossref","first-page":"74","DOI":"10.3389\/fphar.2019.00074","article-title":"Challenges facing airway epithelial cell\u2013based therapy for cystic fibrosis","volume":"10","author":"Berical","year":"2019","journal-title":"Front Pharmacol"},{"key":"2026021100441584000_bib21","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1016\/j.stem.2017.03.001","article-title":"Efficient derivation of functional human airway epithelium from pluripotent stem cells via temporal regulation of Wnt signaling","volume":"20","author":"McCauley","year":"2017","journal-title":"Cell Stem Cell"},{"key":"2026021100441584000_bib22","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1038\/nature24487","article-title":"Regeneration of the entire human epidermis using transgenic stem cells","volume":"551","author":"Hirsch","year":"2017","journal-title":"Nature"},{"key":"2026021100441584000_bib23","first-page":"183","article-title":"Well-differentiated human airway epithelial cell cultures","volume":"107","author":"Fulcher","year":"2005","journal-title":"Methods Mol Med"},{"key":"2026021100441584000_bib24","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1007\/978-1-61779-120-8_18","article-title":"Primary epithelial cell models for cystic fibrosis research","volume":"742","author":"Randell","year":"2011","journal-title":"Methods Mol Biol"},{"key":"2026021100441584000_bib25","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1007\/s13238-018-0506-y","article-title":"Regeneration of functional alveoli by adult human SOX9+ airway basal cell transplantation","volume":"9","author":"Ma","year":"2018","journal-title":"Protein Cell"},{"key":"2026021100441584000_bib26","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1165\/rcmb.2015-0274MA","article-title":"Airway progenitor clone formation is enhanced by Y-27632\u2013dependent changes in the transcriptome","volume":"55","author":"Reynolds","year":"2016","journal-title":"Am J Respir Cell Mol Biol"},{"key":"2026021100441584000_bib27","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1038\/nprot.2016.174","article-title":"Conditional reprogramming and long-term expansion of normal and tumor cells from human biospecimens","volume":"12","author":"Liu","year":"2017","journal-title":"Nat Protoc"},{"key":"2026021100441584000_bib28","doi-asserted-by":"crossref","first-page":"12771","DOI":"10.1073\/pnas.0906850106","article-title":"Basal cells as stem cells of the mouse trachea and human airway epithelium","volume":"106","author":"Rock","year":"2009","journal-title":"Proc Natl Acad Sci USA"},{"key":"2026021100441584000_bib29","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1242\/dmm.006031","article-title":"Airway basal stem cells: a perspective on their roles in epithelial homeostasis and remodeling","volume":"3","author":"Rock","year":"2010","journal-title":"Dis Model Mech"},{"key":"2026021100441584000_bib30","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.celrep.2015.06.011","article-title":"Clonal dynamics reveal two distinct populations of basal cells in slow-turnover airway epithelium","volume":"12","author":"Watson","year":"2015","journal-title":"Cell Rep"},{"key":"2026021100441584000_bib31","first-page":"S288","article-title":"Assessing airway epithelial progenitors for CF cell therapy [abstract]","volume":"52","author":"Miller","year":"2017","journal-title":"Pediatr Pulmonol"},{"key":"2026021100441584000_bib32","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1007\/978-1-62703-125-7_8","article-title":"Human nasal and tracheo-bronchial respiratory epithelial cell culture","volume":"945","author":"Fulcher","year":"2013","journal-title":"Methods Mol Biol"},{"key":"2026021100441584000_bib33","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1165\/rcmb.2016-0276MA","article-title":"Pharmacological rescue of conditionally reprogrammed cystic fibrosis bronchial epithelial cells","volume":"56","author":"Gentzsch","year":"2017","journal-title":"Am J Respir Cell Mol Biol"},{"key":"2026021100441584000_bib34","doi-asserted-by":"crossref","first-page":"20035","DOI":"10.1073\/pnas.1213241109","article-title":"Conditionally reprogrammed cells represent a stem-like state of adult epithelial cells","volume":"109","author":"Suprynowicz","year":"2012","journal-title":"Proc Natl Acad Sci USA"},{"key":"2026021100441584000_bib35","doi-asserted-by":"crossref","first-page":"L550","DOI":"10.1152\/ajplung.00186.2016","article-title":"Restoration of R117H CFTR folding and function in human airway cells through combination treatment with VX-809 and VX-770","volume":"311","author":"Gentzsch","year":"2016","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2026021100441584000_bib36","doi-asserted-by":"crossref","first-page":"L82","DOI":"10.1152\/ajplung.90314.2008","article-title":"Novel human bronchial epithelial cell lines for cystic fibrosis research","volume":"296","author":"Fulcher","year":"2009","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2026021100441584000_bib37","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1164\/rccm.201710-2033OC","article-title":"Chronic e-cigarette exposure alters the human bronchial epithelial proteome","volume":"198","author":"Ghosh","year":"2018","journal-title":"Am J Respir Crit Care Med"},{"key":"2026021100441584000_bib38","doi-asserted-by":"crossref","first-page":"L631","DOI":"10.1152\/ajplung.00112.2003","article-title":"A subset of mouse tracheal epithelial basal cells generates large colonies in vitro","volume":"286","author":"Schoch","year":"2004","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2026021100441584000_bib39","doi-asserted-by":"crossref","first-page":"1948","DOI":"10.1002\/stem.1150","article-title":"Airway epithelial progenitors are region specific and show differential responses to bleomycin-induced lung injury","volume":"30","author":"Chen","year":"2012","journal-title":"Stem Cells"},{"key":"2026021100441584000_bib40","doi-asserted-by":"crossref","first-page":"3025","DOI":"10.1172\/JCI68782","article-title":"Type 2 alveolar cells are stem cells in adult lung","volume":"123","author":"Barkauskas","year":"2013","journal-title":"J Clin Invest"},{"key":"2026021100441584000_bib41","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1007\/978-1-4939-1785-3_17","article-title":"Clonal culture of adult mouse lung epithelial stem\/progenitor cells","volume":"1235","author":"McQualter","year":"2015","journal-title":"Methods Mol Biol"},{"key":"2026021100441584000_bib42","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1038\/s41586-018-0393-7","article-title":"A revised airway epithelial hierarchy includes CFTR-expressing ionocytes","volume":"560","author":"Montoro","year":"2018","journal-title":"Nature"},{"key":"2026021100441584000_bib43","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1038\/s41586-018-0394-6","article-title":"A single-cell atlas of the airway epithelium reveals the CFTR-rich pulmonary ionocyte","volume":"560","author":"Plasschaert","year":"2018","journal-title":"Nature"},{"key":"2026021100441584000_bib44","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1016\/j.ccr.2014.03.033","article-title":"Loss of Lkb1 and Pten leads to lung squamous cell carcinoma with elevated PD-L1 expression","volume":"25","author":"Xu","year":"2014","journal-title":"Cancer Cell"},{"key":"2026021100441584000_bib45","doi-asserted-by":"crossref","first-page":"146","DOI":"10.3109\/01902148.2013.781720","article-title":"Isolation of fibroblasts and epithelial cells in bronchoalveolar lavage (BAL)","volume":"39","author":"Pollock","year":"2013","journal-title":"Exp Lung Res"},{"key":"2026021100441584000_bib46","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.stem.2016.05.012","article-title":"Dual SMAD signaling inhibition enables long-term expansion of diverse epithelial basal cells","volume":"19","author":"Mou","year":"2016","journal-title":"Cell Stem Cell"},{"key":"2026021100441584000_bib47","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1164\/rccm.201507-1414OC","article-title":"Rapid expansion of human epithelial stem cells suitable for airway tissue engineering","volume":"194","author":"Butler","year":"2016","journal-title":"Am J Respir Crit Care Med"},{"key":"2026021100441584000_bib48","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1038\/mt.2009.96","article-title":"Engraftment of bone marrow-derived stem cells to the lung in a model of acute respiratory infection by Pseudomonas aeruginosa","volume":"17","author":"Rejman","year":"2009","journal-title":"Mol Ther"},{"key":"2026021100441584000_bib49","first-page":"336","article-title":"Identification of a bone marrow\u2013derived epithelial-like population capable of repopulating injured mouse airway epithelium","volume":"119","author":"Wong","year":"2009","journal-title":"J Clin Invest"},{"key":"2026021100441584000_bib50","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.1089\/hum.2009.035","article-title":"Developing cell therapy techniques for respiratory disease: intratracheal delivery of genetically engineered stem cells in a murine model of airway injury","volume":"20","author":"Leblond","year":"2009","journal-title":"Hum Gene Ther"},{"key":"2026021100441584000_bib51","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1038\/nnano.2013.147","article-title":"Tracking the engraftment and regenerative capabilities of transplanted lung stem cells using fluorescent nanodiamonds","volume":"8","author":"Wu","year":"2013","journal-title":"Nat Nanotechnol"},{"key":"2026021100441584000_bib52","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1038\/nm.3889","article-title":"Preconditioning allows engraftment of mouse and human embryonic lung cells, enabling lung repair in mice","volume":"21","author":"Rosen","year":"2015","journal-title":"Nat Med"},{"key":"2026021100441584000_bib53","doi-asserted-by":"crossref","first-page":"1830","DOI":"10.1038\/mt.2010.142","article-title":"Optimization of targeted cell replacement therapy: a new approach for lung disease","volume":"18","author":"Duchesneau","year":"2010","journal-title":"Mol Ther"},{"key":"2026021100441584000_bib54","doi-asserted-by":"crossref","first-page":"L221","DOI":"10.1152\/ajplung.00147.2014","article-title":"Efficient intratracheal delivery of airway epithelial cells in mice and pigs","volume":"308","author":"Gui","year":"2015","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2026021100441584000_bib55","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1165\/rcmb.2013-0046TE","article-title":"Rho-associated protein kinase inhibition enhances airway epithelial basal-cell proliferation and lentivirus transduction","volume":"49","author":"Horani","year":"2013","journal-title":"Am J Respir Cell Mol Biol"},{"key":"2026021100441584000_bib56","doi-asserted-by":"crossref","first-page":"17971","DOI":"10.1038\/s41598-017-17952-4","article-title":"Conditionally reprogrammed primary airway epithelial cells maintain morphology, lineage and disease specific functional characteristics","volume":"7","author":"Martinovich","year":"2017","journal-title":"Sci Rep"},{"key":"2026021100441584000_bib57","doi-asserted-by":"crossref","first-page":"L313","DOI":"10.1152\/ajplung.00355.2017","article-title":"Long-term culture and cloning of primary human bronchial basal cells that maintain multipotent differentiation capacity and CFTR channel function","volume":"315","author":"Peters-Hall","year":"2018","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2026021100441584000_bib58","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1016\/j.celrep.2018.09.072","article-title":"Long-term in vitro expansion of epithelial stem cells enabled by pharmacological inhibition of PAK1-ROCK-myosin II and TGF-\u03b2 signaling","volume":"25","author":"Zhang","year":"2018","journal-title":"Cell Rep"},{"key":"2026021100441584000_bib59","doi-asserted-by":"crossref","first-page":"1390","DOI":"10.1183\/09031936.00221711","article-title":"Improvement of defective cystic fibrosis airway epithelial wound repair after CFTR rescue","volume":"40","author":"Trinh","year":"2012","journal-title":"Eur Respir J"},{"key":"2026021100441584000_bib60","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1038\/s41374-018-0026-7","article-title":"Lack of cystic fibrosis transmembrane conductance regulator disrupts fetal airway development in pigs","volume":"98","author":"Meyerholz","year":"2018","journal-title":"Lab Invest"},{"key":"2026021100441584000_bib61","doi-asserted-by":"crossref","first-page":"L4","DOI":"10.1152\/ajplung.00372.2005","article-title":"Improvement of pulmonary hypoplasia associated with congenital diaphragmatic hernia by in utero CFTR gene therapy","volume":"291","author":"Larson","year":"2006","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2026021100441584000_bib62","doi-asserted-by":"crossref","first-page":"L333","DOI":"10.1152\/ajplung.2000.279.2.L333","article-title":"CFTR modulates lung secretory cell proliferation and differentiation","volume":"279","author":"Larson","year":"2000","journal-title":"Am J Physiol Lung Cell Mol Physiol"},{"key":"2026021100441584000_bib63","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1002\/sctm.18-0098","article-title":"Cell therapy for cystic fibrosis lung disease: regenerative basal cell amplification","volume":"8","author":"Hayes","year":"2019","journal-title":"Stem Cells Transl Med"},{"key":"2026021100441584000_bib64","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1038\/ng0992-21","article-title":"Efficiency of gene transfer for restoration of normal airway epithelial function in cystic fibrosis","volume":"2","author":"Johnson","year":"1992","journal-title":"Nat Genet"},{"key":"2026021100441584000_bib65","doi-asserted-by":"crossref","first-page":"e83624","DOI":"10.1371\/journal.pone.0083624","article-title":"Integrin \u03b16\u03b24 identifies human distal lung epithelial progenitor cells with potential as a cell-based therapy for cystic fibrosis lung disease","volume":"8","author":"Li","year":"2013","journal-title":"PLoS One"},{"key":"2026021100441584000_bib66","doi-asserted-by":"crossref","first-page":"e1000155","DOI":"10.1371\/journal.pbio.1000155","article-title":"CFTR delivery to 25% of surface epithelial cells restores normal rates of mucus transport to human cystic fibrosis airway epithelium","volume":"7","author":"Zhang","year":"2009","journal-title":"PLoS Biol"},{"key":"2026021100441584000_bib67","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1016\/j.stem.2018.03.017","article-title":"Submucosal gland myoepithelial cells are reserve stem cells that can regenerate mouse tracheal epithelium","volume":"22","author":"Lynch","year":"2018","journal-title":"Cell Stem Cell"},{"key":"2026021100441584000_bib68","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1016\/j.stem.2018.03.018","article-title":"Myoepithelial cells of submucosal glands can function as reserve stem cells to regenerate airways after injury","volume":"22","author":"Tata","year":"2018","journal-title":"Cell Stem Cell"}],"container-title":["American Journal of Respiratory Cell and Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/63\/3\/374\/66765181\/ajrcmb_63_3_374.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/63\/3\/374\/66765181\/ajrcmb_63_3_374.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T05:44:33Z","timestamp":1770788673000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article\/63\/3\/374\/8461184"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,1]]},"references-count":68,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2020,9,1]]},"published-print":{"date-parts":[[2020,9,1]]}},"URL":"https:\/\/doi.org\/10.1165\/rcmb.2019-0384oc","relation":{},"ISSN":["1044-1549","1535-4989"],"issn-type":[{"value":"1044-1549","type":"print"},{"value":"1535-4989","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,9]]},"published":{"date-parts":[[2020,9,1]]},"assertion":[{"value":"2019-10-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-05-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}