{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T11:42:06Z","timestamp":1777290126857,"version":"3.51.4"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["www.atsjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Aspergillus fumigatus is an opportunistic fungal pathogen responsible for a spectrum of clinical manifestations. Dendritic cells recognize pathogen-associated molecular patterns of Aspergillus via two main receptor families, Toll-like receptors (TLRs) and C-type lectin receptors (CLR). Here, the importance of TLR and CLR signaling in the regulation of T-helper cell type 2 (Th2) responses was analyzed using a mouse model based on the transfer of bone marrow\u2013derived dendritic cells (BMDCs) pulsed with A. fumigatus conidia. BMDCs were generated from mice deficient in either MyD88 or MALT1 (mucosa-associated lymphoid tissue lymphoma translocation protein 1). Both the MyD88 and MALT1 signaling pathway in BMDCs contributed to the production of inflammatory cytokines induced by A. fumigatus conidia. Mice sensitized with MyD88\u2212\/\u2212 BMDCs pulsed in vitro with A. fumigatus conidia showed an exacerbated allergic inflammation, with stronger eosinophil recruitment in the BAL and higher Th2 cytokine production compared with mice sensitized with wild-type or MALT1\u2212\/\u2212 BMDCs. This exacerbation was not observed when MyD88\u2212\/\u2212 BMDCs were pulsed with Cladosporium sphaerospermum, a nonpathogenic mold. A lack of TLR2 signaling recapitulated the exacerbation of the A. fumigatus Th2 response observed in the absence of MyD88 signaling, whereas TLR2 agonist dampened the response induced with A. fumigatus and C. sphaerospermum conidia. IL-10 production by BMDCs in response to A. fumigatus was dependent on the expression of TLR2 and MyD88. IL-10\u2212\/\u2212 BMDCs exacerbated, whereas MyD88\u2212\/\u2212 BMDCs supplemented with exogenous IL-10 decreased the allergic pulmonary inflammation. These results indicate that TLR2\/MyD88-specific recognition of PAMPs from A. fumigatus conidia can upregulate IL-10 production and downregulate lung eosinophilia and the development of a Th2 response.<\/jats:p>","DOI":"10.1165\/rcmb.2020-0083oc","type":"journal-article","created":{"date-parts":[[2020,9,24]],"date-time":"2020-09-24T17:46:45Z","timestamp":1600969605000},"page":"39-49","update-policy":"https:\/\/doi.org\/10.1164\/crossmarkpolicies","source":"Crossref","is-referenced-by-count":17,"title":["<i>Aspergillus<\/i>\n                    \u00a0\n                    <i>Fumigatus<\/i>\n                    Recognition by Dendritic Cells Negatively Regulates Allergic Lung Inflammation through a TLR2\/MyD88 Pathway"],"prefix":"10.1093","volume":"64","author":[{"given":"Pauline","family":"Percier","sequence":"first","affiliation":[{"name":"Service Immune Response and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sofie","family":"De Prins","sequence":"additional","affiliation":[{"name":"Service Immune Response and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giresse","family":"Tima","sequence":"additional","affiliation":[{"name":"Service Viral Diseases, Sciensano, Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rudi","family":"Beyaert","sequence":"additional","affiliation":[{"name":"Center for Inflammation Research, Unit of Molecular Signal Transduction in Inflammation, Vlaams Instituut voor Biotechnologie, Ghent, Belgium"},{"name":"Department for Biomedical Molecular Biology, Ghent University, Ghent, Belgium; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johan","family":"Grooten","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunology, Department of Biomedical Molecular Biology, Ghent University, Zwijnaarde, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Romano","sequence":"additional","affiliation":[{"name":"Service Immune Response and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1200-7552","authenticated-orcid":false,"given":"Olivier","family":"Denis","sequence":"additional","affiliation":[{"name":"Service Immune Response and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,1,1]]},"reference":[{"key":"2026021100472213000_bib1","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1128\/CMR.12.2.310","article-title":"Aspergillus fumigatus and aspergillosis","volume":"12","author":"Latg\u00e9","year":"1999","journal-title":"Clin Microbiol Rev"},{"key":"2026021100472213000_bib2","doi-asserted-by":"crossref","first-page":"693023","DOI":"10.1155\/2013\/693023","article-title":"The immune interplay between the host and the pathogen in 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