{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T08:03:55Z","timestamp":1793347435560,"version":"4.5.0"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T00:00:00Z","timestamp":1701388800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HL122531"],"award-info":[{"award-number":["R01HL122531"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P01GM0955467"],"award-info":[{"award-number":["P01GM0955467"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R35GM139430"],"award-info":[{"award-number":["R35GM139430"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HL122531"],"award-info":[{"award-number":["R01HL122531"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P01GM0955467"],"award-info":[{"award-number":["P01GM0955467"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R35GM139430"],"award-info":[{"award-number":["R35GM139430"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.atsjournals.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Eosinophils (Eos) reside in multiple organs during homeostasis and respond rapidly to an inflammatory challenge. Although Eos share chemical staining properties, they also demonstrate phenotypic and functional plasticity that is not fully understood. Here, we used a murine model of allergic lung inflammation to characterize Eos subsets and determine their spatiotemporal and functional regulation during inflammation and its resolution in response to resolvin D2 (RvD2), a potent specialized proresolving mediator. Two Eos subsets were identified by CD101 expression with distinct anatomic localization and transcriptional signatures at baseline and during inflammation. CD101low Eos were predominantly located in a lung vascular niche and responded to allergen challenge by moving into the lung interstitium. CD101high Eos were predominantly located in bronchoalveolar lavage (BAL) and extravascular lung, only present during inflammation, and had transcriptional evidence for cell activation. RvD2 reduced total Eos numbers and changed their phenotype and activation by at least two distinct mechanisms: decreasing interleukin 5-dependent recruitment of CD101low Eos and decreasing conversion of CD101low Eos to CD101high Eos. Collectively, these findings indicate that Eos are a heterogeneous pool of cells with distinct activation states and spatiotemporal regulation during resolution of inflammation and that RvD2 is a potent proresolving mediator for Eos recruitment and activation.<\/jats:p>","DOI":"10.1165\/rcmb.2023-0121oc","type":"journal-article","created":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T15:43:37Z","timestamp":1691509417000},"page":"666-677","update-policy":"https:\/\/doi.org\/10.1164\/crossmarkpolicies","source":"Crossref","is-referenced-by-count":16,"title":["Eosinophil Phenotypes Are Functionally Regulated by Resolvin D2 during Allergic Lung Inflammation"],"prefix":"10.1093","volume":"69","author":[{"given":"Thayse R.","family":"Br\u00fcggemann","sequence":"first","affiliation":[{"name":"Pulmonary and Critical Care Medicine, Department of Internal Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong Yong","family":"Peh","sequence":"additional","affiliation":[{"name":"Pulmonary and Critical Care Medicine, Department of Internal Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana P.","family":"Tavares","sequence":"additional","affiliation":[{"name":"Pulmonary and Critical Care Medicine, Department of Internal Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Nijmeh","sequence":"additional","affiliation":[{"name":"Pulmonary and Critical Care Medicine, Department of Internal Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashley E.","family":"Shay","sequence":"additional","affiliation":[{"name":"Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Perioperative and Pain Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael M.","family":"Rezende","sequence":"additional","affiliation":[{"name":"Ann Romney Center for Neurologic Diseases, Brigham and Women\u2019s Hospital, Harvard Medical School, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toby B.","family":"Lanser","sequence":"additional","affiliation":[{"name":"Ann Romney Center for Neurologic Diseases, Brigham and Women\u2019s Hospital, Harvard Medical School, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles N.","family":"Serhan","sequence":"additional","affiliation":[{"name":"Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Perioperative and Pain Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9515-5731","authenticated-orcid":false,"given":"Bruce D.","family":"Levy","sequence":"additional","affiliation":[{"name":"Pulmonary and Critical Care Medicine, Department of Internal Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,12,1]]},"reference":[{"key":"2026012204055808700_bib1","doi-asserted-by":"crossref","first-page":"4425","DOI":"10.1038\/s41598-020-61420-5","article-title":"Eosinophil accumulation in postnatal lung is specific to the primary septation phase of development","volume":"10","author":"Loffredo","year":"2020","journal-title":"Sci Rep"},{"key":"2026012204055808700_bib2","doi-asserted-by":"crossref","first-page":"2694","DOI":"10.1016\/j.mayocp.2021.04.025","article-title":"Eosinophils in health and disease: a state-of-the-art review","volume":"96","author":"Wechsler","year":"2021","journal-title":"Mayo Clin Proc"},{"key":"2026012204055808700_bib3","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.1056\/NEJMra0804579","article-title":"Asthma","volume":"360","author":"Fanta","year":"2009","journal-title":"N Engl J Med"},{"key":"2026012204055808700_bib4","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1146\/annurev-immunol-093019-125918","article-title":"Eosinophil knockout humans: uncovering the role of eosinophils through eosinophil-directed biological therapies","volume":"39","author":"Jacobsen","year":"2021","journal-title":"Annu Rev Immunol"},{"key":"2026012204055808700_bib5","doi-asserted-by":"crossref","DOI":"10.1128\/microbiolspec.MCHD-0032-2016","article-title":"Paul Ehrlich and the early history of granulocytes","volume":"4","author":"Kay","year":"2016","journal-title":"Microbiol Spectr"},{"key":"2026012204055808700_bib6","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1002\/JLB.1MR1117-442RR","article-title":"Shaping eosinophil identity in the tissue contexts of development, homeostasis, and disease","volume":"104","author":"Abdala-Valencia","year":"2018","journal-title":"J Leukoc Biol"},{"key":"2026012204055808700_bib7","doi-asserted-by":"crossref","first-page":"3279","DOI":"10.1172\/JCI85664","article-title":"Lung-resident eosinophils represent a distinct regulatory eosinophil subset","volume":"126","author":"Mesnil","year":"2016","journal-title":"J Clin Invest"},{"key":"2026012204055808700_bib8","doi-asserted-by":"crossref","first-page":"3879","DOI":"10.1038\/s41467-018-06316-9","article-title":"Eosinophil recruitment is dynamically regulated by interplay among lung dendritic cell subsets after allergen challenge","volume":"9","author":"Yi","year":"2018","journal-title":"Nat Commun"},{"key":"2026012204055808700_bib9","doi-asserted-by":"crossref","first-page":"270","DOI":"10.3389\/fimmu.2012.00270","article-title":"Emerging roles of eosinophils and eosinophil-derived lipid mediators in the resolution of inflammation","volume":"3","author":"Isobe","year":"2012","journal-title":"Front Immunol"},{"key":"2026012204055808700_bib10","first-page":"CD010834","article-title":"Anti-IL-5 therapies for asthma","volume":"7","author":"Farne","year":"2022","journal-title":"Cochrane Database Syst Rev"},{"key":"2026012204055808700_bib11","first-page":"305","article-title":"Inhibition of interleukin-5 for the treatment of eosinophilic diseases","volume":"13","author":"Corren","year":"2012","journal-title":"Discov Med"},{"key":"2026012204055808700_bib12","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.1164\/rccm.201611-2234OC","article-title":"Mepolizumab attenuates airway eosinophil numbers, but not their functional phenotype, in asthma","volume":"196","author":"Kelly","year":"2017","journal-title":"Am J Respir Crit Care Med"},{"key":"2026012204055808700_bib13","doi-asserted-by":"crossref","first-page":"1335","DOI":"10.1152\/physrev.00026.2017","article-title":"Specialized proresolving mediators in innate and adaptive immune responses in airway diseases","volume":"98","author":"Krishnamoorthy","year":"2018","journal-title":"Physiol Rev"},{"key":"2026012204055808700_bib14","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1084\/jem.20020760","article-title":"Resolvins: a family of bioactive products of omega-3 fatty acid transformation circuits initiated by aspirin treatment that counter proinflammation signals","volume":"196","author":"Serhan","year":"2002","journal-title":"J Exp Med"},{"key":"2026012204055808700_bib15","doi-asserted-by":"crossref","first-page":"700","DOI":"10.1038\/s42003-021-02227-6","article-title":"Prioritization of candidate causal genes for asthma in susceptibility loci derived from UK Biobank","volume":"4","author":"Valette","year":"2021","journal-title":"Commun Biol"},{"key":"2026012204055808700_bib16","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/0014-5793(87)80671-3","article-title":"Formation of lipoxin A by granulocytes from eosinophilic donors","volume":"217","author":"Serhan","year":"1987","journal-title":"FEBS Lett"},{"key":"2026012204055808700_bib17","doi-asserted-by":"crossref","first-page":"S2","DOI":"10.1016\/j.alit.2016.05.010","article-title":"Eosinophil polyunsaturated fatty acid metabolism and its potential control of inflammation and allergy","volume":"65","author":"Arita","year":"2016","journal-title":"Allergol Int"},{"key":"2026012204055808700_bib18","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1164\/rccm.200410-1413OC","article-title":"Diminished lipoxin biosynthesis in severe asthma","volume":"172","author":"Levy","year":"2005","journal-title":"Am J Respir Crit Care Med"},{"key":"2026012204055808700_bib19","doi-asserted-by":"crossref","first-page":"496","DOI":"10.4049\/jimmunol.178.1.496","article-title":"Protectin D1 is generated in asthma and dampens airway inflammation and hyperresponsiveness","volume":"178","author":"Levy","year":"2007","journal-title":"J Immunol"},{"key":"2026012204055808700_bib20","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/j.jaci.2012.07.048","article-title":"Dysregulated synthesis of protectin D1 in eosinophils from patients with severe asthma","volume":"131","author":"Miyata","year":"2013","journal-title":"J Allergy Clin Immunol"},{"key":"2026012204055808700_bib21","doi-asserted-by":"crossref","first-page":"4004","DOI":"10.4049\/jimmunol.181.6.4004","article-title":"Functionally competent eosinophils differentiated ex vivo in high purity from normal mouse bone marrow","volume":"181","author":"Dyer","year":"2008","journal-title":"J Immunol"},{"key":"2026012204055808700_bib22","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1111\/all.12776","article-title":"Phenotypic plasticity and targeting of Siglec-Fhigh CD11clow eosinophils to the airway in a murine model of asthma","volume":"71","author":"Abdala Valencia","year":"2016","journal-title":"Allergy"},{"key":"2026012204055808700_bib23","doi-asserted-by":"crossref","first-page":"1902354","DOI":"10.1183\/13993003.02354-2019","article-title":"Homeostatic and early-recruited CD101- eosinophils suppress endotoxin-induced acute lung injury","volume":"56","author":"Zhu","year":"2020","journal-title":"Eur Respir J"},{"key":"2026012204055808700_bib24","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1084\/jem.20150225","article-title":"Identification of resolvin D2 receptor mediating resolution of infections and organ protection","volume":"212","author":"Chiang","year":"2015","journal-title":"J Exp Med"},{"key":"2026012204055808700_bib25","doi-asserted-by":"crossref","first-page":"4345","DOI":"10.4049\/jimmunol.174.7.4345","article-title":"Molecular circuits of resolution: formation and actions of resolvins and protectins","volume":"174","author":"Bannenberg","year":"2005","journal-title":"J Immunol"},{"key":"2026012204055808700_bib26","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1038\/89759","article-title":"Lipid mediator class switching during acute inflammation: signals in resolution","volume":"2","author":"Levy","year":"2001","journal-title":"Nat Immunol"},{"key":"2026012204055808700_bib27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1189\/jlb.0607344","article-title":"Roles of integrin activation in eosinophil function and the eosinophilic inflammation of asthma","volume":"83","author":"Barthel","year":"2008","journal-title":"J Leukoc Biol"},{"key":"2026012204055808700_bib28","doi-asserted-by":"crossref","first-page":"15025","DOI":"10.1038\/nrdp.2015.25","article-title":"Asthma","volume":"1","author":"Holgate","year":"2015","journal-title":"Nat Rev Dis Primers"},{"key":"2026012204055808700_bib29","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1038\/mi.2015.139","article-title":"CD101 inhibits the expansion of colitogenic T cells","volume":"9","author":"Schey","year":"2016","journal-title":"Mucosal Immunol"},{"key":"2026012204055808700_bib30","doi-asserted-by":"crossref","first-page":"5393","DOI":"10.4049\/jimmunol.0803874","article-title":"Ym1\/2 promotes Th2 cytokine expression by inhibiting 12\/15(S)-lipoxygenase: identification of a novel pathway for regulating allergic inflammation","volume":"182","author":"Cai","year":"2009","journal-title":"J Immunol"},{"key":"2026012204055808700_bib31","doi-asserted-by":"crossref","first-page":"5105","DOI":"10.1038\/s41598-018-23269-7","article-title":"Dual role of YM1+ M2 macrophages in allergic lung inflammation","volume":"8","author":"Draijer","year":"2018","journal-title":"Sci Rep"},{"key":"2026012204055808700_bib32","doi-asserted-by":"crossref","first-page":"42821","DOI":"10.1074\/jbc.M205873200","article-title":"TH2 cytokines and allergic challenge induce Ym1 expression in macrophages by a STAT6-dependent mechanism","volume":"277","author":"Welch","year":"2002","journal-title":"J Biol Chem"},{"key":"2026012204055808700_bib33","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1186\/rr41","article-title":"Th2 cytokines and asthma. The role of interleukin-5 in allergic eosinophilic disease","volume":"2","author":"Greenfeder","year":"2001","journal-title":"Respir Res"},{"key":"2026012204055808700_bib34","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1007\/BF03256653","article-title":"Anti-interleukin-5 monoclonal antibodies: preclinical and clinical evidence in asthma models","volume":"2","author":"Leckie","year":"2003","journal-title":"Am J Respir Med"}],"container-title":["American Journal of Respiratory Cell and Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/69\/6\/666\/66296333\/ajrcmb_69_6_666.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article-pdf\/69\/6\/666\/66296333\/ajrcmb_69_6_666.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T09:12:01Z","timestamp":1769073121000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ajrcmb\/article\/69\/6\/666\/8416677"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,1]]},"references-count":34,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,12,1]]},"published-print":{"date-parts":[[2023,12,1]]}},"URL":"https:\/\/doi.org\/10.1165\/rcmb.2023-0121oc","relation":{},"ISSN":["1044-1549","1535-4989"],"issn-type":[{"value":"1044-1549","type":"print"},{"value":"1535-4989","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2023,12]]},"published":{"date-parts":[[2023,12,1]]},"assertion":[{"value":"2023-03-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}