{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T00:16:56Z","timestamp":1772929016574,"version":"3.50.1"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2006,7,1]],"date-time":"2006-07-01T00:00:00Z","timestamp":1151712000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>A panel of 11 human cystic fibrosis transmembrane conductance regulator (hCFTR) antibodies were tested in ovine nasal, tracheal, and bronchial epithelial brushings. Two of these, G449 (polyclonal) and MATG1104 (monoclonal), recognized hCFTR but did not cross react with endogenous sheep CFTR. This specificity allows immunologic detection of hCFTR expressed in gene transfer studies in sheep against the background of endogenous ovine CFTR, thus enhancing the value of the sheep as a model animal in which to study CFTR gene transfer. Studies on mixed populations of human and sheep nasal epithelial cells showed that detection of hCFTR by these two antibodies was possible even at the lowest proportion of human cells (1:100). The hCFTR gene was delivered in vivo by local instillation using polyethylenimine-mediated gene transfer to the ventral surface of the ovine trachea and hCFTR mRNA and protein levels scored in a blinded fashion. Despite abundant hCFTR mRNA expression, the number of cells expressing hCFTR protein detectable by G449 was low (\u223c 0.006\u20130.05%). Immunohistochemistry for hCFTR in animals treated by whole-lung aerosol demonstrated positive cells in sections of tracheal epithelium and in distal conducting airways. The strategic use of hCFTR-specific antibodies supports the utility of the normal sheep as a model for hCFTR gene transfer studies.<\/jats:p>","DOI":"10.1165\/rcmb.2005-0377oc","type":"journal-article","created":{"date-parts":[[2006,2,23]],"date-time":"2006-02-23T20:59:50Z","timestamp":1140728390000},"page":"72-83","source":"Crossref","is-referenced-by-count":9,"title":["Human-Specific Cystic Fibrosis Transmembrane Conductance Regulator Antibodies Detect\n                    <i>In Vivo<\/i>\n                    Gene Transfer to Ovine Airways"],"prefix":"10.1093","volume":"35","author":[{"given":"Heather","family":"Davidson","sequence":"first","affiliation":[{"name":"Medical Sciences (Medical Genetics), University of Edinburgh, Western General Hospital, Edinburgh; The Wellcome Trust Centre in Comparative Respiratory Medicine, Easter Bush Veterinary Centre, University of Edinburgh, Roslin; Gene Medicine Group, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford; UK Cystic Fibrosis Gene Therapy Consortium, London, Oxford, and Edinburgh, United Kingdom; Centro de Gen\u00e9tica Humana, Instituto Nacional Sa\u00fade Dr Ricardo Jorge; and Department of Chemistry and Biochemistry, Faculty of Sciences, University of Lisbon, Lisbon, Portugal"}]},{"given":"Gerry","family":"McLachlan","sequence":"additional","affiliation":[{"name":"Medical Sciences (Medical Genetics), University of Edinburgh, Western General Hospital, Edinburgh; The Wellcome Trust Centre in Comparative Respiratory Medicine, Easter Bush Veterinary Centre, University of Edinburgh, Roslin; Gene Medicine Group, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford; UK Cystic Fibrosis Gene Therapy Consortium, London, Oxford, and Edinburgh, United Kingdom; Centro de Gen\u00e9tica Humana, Instituto Nacional Sa\u00fade Dr Ricardo Jorge; and Department of Chemistry and Biochemistry, Faculty of Sciences, University of Lisbon, Lisbon, Portugal"}]},{"given":"Abigail","family":"Wilson","sequence":"additional","affiliation":[{"name":"Medical Sciences (Medical Genetics), University of Edinburgh, Western General Hospital, Edinburgh; The Wellcome Trust Centre in Comparative Respiratory Medicine, Easter Bush Veterinary Centre, University of Edinburgh, Roslin; Gene Medicine Group, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford; UK Cystic Fibrosis Gene Therapy Consortium, London, Oxford, and Edinburgh, United Kingdom; Centro de Gen\u00e9tica Humana, Instituto Nacional Sa\u00fade Dr Ricardo Jorge; and Department of Chemistry and Biochemistry, Faculty of Sciences, University of Lisbon, Lisbon, Portugal"}]},{"given":"A. Christopher","family":"Boyd","sequence":"additional","affiliation":[{"name":"Medical Sciences (Medical Genetics), University of Edinburgh, Western General Hospital, Edinburgh; The Wellcome Trust Centre in Comparative Respiratory Medicine, Easter Bush Veterinary Centre, University of Edinburgh, Roslin; Gene Medicine Group, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford; UK Cystic Fibrosis Gene Therapy Consortium, London, Oxford, and Edinburgh, United Kingdom; Centro de Gen\u00e9tica Humana, Instituto Nacional Sa\u00fade Dr Ricardo Jorge; and Department of Chemistry and Biochemistry, Faculty of Sciences, University of Lisbon, Lisbon, Portugal"}]},{"given":"Ann","family":"Doherty","sequence":"additional","affiliation":[{"name":"Medical Sciences (Medical Genetics), University of Edinburgh, Western General Hospital, Edinburgh; The Wellcome Trust Centre in Comparative Respiratory Medicine, Easter Bush Veterinary Centre, University of Edinburgh, Roslin; Gene Medicine Group, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford; UK Cystic Fibrosis Gene Therapy Consortium, London, Oxford, and Edinburgh, United Kingdom; Centro de Gen\u00e9tica Humana, Instituto Nacional Sa\u00fade Dr Ricardo Jorge; and Department of Chemistry and Biochemistry, Faculty of Sciences, University of Lisbon, Lisbon, Portugal"}]},{"given":"Gordon","family":"MacGregor","sequence":"additional","affiliation":[{"name":"Medical Sciences (Medical Genetics), University of Edinburgh, Western General Hospital, Edinburgh; The Wellcome Trust Centre in Comparative Respiratory Medicine, Easter Bush Veterinary Centre, University of Edinburgh, Roslin; Gene Medicine Group, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford; UK Cystic Fibrosis Gene Therapy Consortium, London, Oxford, and Edinburgh, United Kingdom; Centro de Gen\u00e9tica Humana, Instituto Nacional Sa\u00fade Dr Ricardo Jorge; and Department of Chemistry and Biochemistry, Faculty of Sciences, University of Lisbon, Lisbon, Portugal"}]},{"given":"Lee","family":"Davies","sequence":"additional","affiliation":[{"name":"Medical Sciences (Medical Genetics), University of Edinburgh, Western General Hospital, Edinburgh; The Wellcome Trust Centre in Comparative Respiratory Medicine, Easter Bush Veterinary Centre, University of Edinburgh, Roslin; Gene Medicine Group, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford; UK Cystic Fibrosis Gene Therapy Consortium, London, Oxford, and Edinburgh, United Kingdom; Centro de Gen\u00e9tica Humana, Instituto Nacional Sa\u00fade Dr Ricardo Jorge; and Department of Chemistry and Biochemistry, Faculty of Sciences, University of Lisbon, Lisbon, Portugal"}]},{"given":"Hazel A.","family":"Painter","sequence":"additional","affiliation":[{"name":"Medical Sciences (Medical Genetics), University of Edinburgh, Western General Hospital, Edinburgh; The Wellcome Trust Centre in Comparative Respiratory Medicine, Easter Bush Veterinary Centre, University of Edinburgh, Roslin; Gene Medicine Group, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford; UK Cystic Fibrosis Gene Therapy Consortium, London, Oxford, and Edinburgh, United Kingdom; Centro de Gen\u00e9tica Humana, Instituto Nacional Sa\u00fade Dr Ricardo Jorge; and Department of Chemistry and Biochemistry, Faculty of Sciences, University of Lisbon, Lisbon, Portugal"}]},{"given":"Rebecca","family":"Coles","sequence":"additional","affiliation":[{"name":"Medical Sciences (Medical Genetics), University of Edinburgh, Western General Hospital, Edinburgh; The Wellcome Trust Centre in Comparative Respiratory Medicine, Easter Bush Veterinary Centre, University of Edinburgh, Roslin; Gene Medicine Group, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford; UK Cystic Fibrosis Gene Therapy Consortium, London, Oxford, and Edinburgh, United Kingdom; Centro de Gen\u00e9tica Humana, Instituto Nacional Sa\u00fade Dr Ricardo Jorge; and Department of Chemistry and Biochemistry, Faculty of Sciences, University of Lisbon, Lisbon, Portugal"}]},{"given":"Stephen C.","family":"Hyde","sequence":"additional","affiliation":[{"name":"Medical Sciences (Medical Genetics), University of Edinburgh, Western General Hospital, Edinburgh; The Wellcome Trust Centre in Comparative Respiratory Medicine, Easter Bush Veterinary Centre, University of Edinburgh, Roslin; Gene Medicine Group, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford; UK Cystic Fibrosis Gene Therapy Consortium, London, Oxford, and Edinburgh, United Kingdom; Centro de Gen\u00e9tica Humana, Instituto Nacional Sa\u00fade Dr Ricardo Jorge; and Department of Chemistry and Biochemistry, Faculty of Sciences, University of Lisbon, Lisbon, Portugal"}]},{"given":"Deborah R.","family":"Gill","sequence":"additional","affiliation":[{"name":"Medical Sciences (Medical Genetics), University of Edinburgh, Western General Hospital, Edinburgh; The Wellcome Trust Centre in Comparative Respiratory Medicine, Easter Bush Veterinary Centre, University of Edinburgh, Roslin; Gene Medicine Group, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford; UK Cystic Fibrosis Gene Therapy Consortium, London, Oxford, and Edinburgh, United Kingdom; Centro de Gen\u00e9tica Humana, Instituto Nacional Sa\u00fade Dr Ricardo Jorge; and Department of Chemistry and Biochemistry, Faculty of Sciences, University of Lisbon, Lisbon, Portugal"}]},{"given":"Margarida D.","family":"Amaral","sequence":"additional","affiliation":[{"name":"Medical Sciences (Medical Genetics), University of Edinburgh, Western General Hospital, Edinburgh; The Wellcome Trust Centre in Comparative Respiratory Medicine, Easter Bush Veterinary Centre, University of Edinburgh, Roslin; Gene Medicine Group, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford; UK Cystic Fibrosis Gene Therapy Consortium, London, Oxford, and Edinburgh, United Kingdom; Centro de Gen\u00e9tica Humana, Instituto Nacional Sa\u00fade Dr Ricardo Jorge; and Department of Chemistry and Biochemistry, Faculty of Sciences, University of Lisbon, Lisbon, Portugal"}]},{"given":"David D. S.","family":"Collie","sequence":"additional","affiliation":[{"name":"Medical Sciences (Medical Genetics), University of Edinburgh, Western General Hospital, Edinburgh; The Wellcome Trust Centre in Comparative Respiratory Medicine, Easter Bush Veterinary Centre, University of Edinburgh, Roslin; Gene Medicine Group, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford; UK Cystic Fibrosis Gene Therapy Consortium, London, Oxford, and Edinburgh, United Kingdom; Centro de Gen\u00e9tica Humana, Instituto Nacional Sa\u00fade Dr Ricardo Jorge; and Department of Chemistry and Biochemistry, Faculty of Sciences, University of Lisbon, Lisbon, Portugal"}]},{"given":"David J.","family":"Porteous","sequence":"additional","affiliation":[{"name":"Medical Sciences (Medical Genetics), University of Edinburgh, Western General Hospital, Edinburgh; The Wellcome Trust Centre in Comparative Respiratory Medicine, Easter Bush Veterinary Centre, University of 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