{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T23:42:28Z","timestamp":1787614948547,"version":"build-2736575974"},"reference-count":0,"publisher":"American Scientific Publishers","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["j biomed nanotechnol"],"published-print":{"date-parts":[[2020,3,1]]},"abstract":"<jats:p>New biomarkers have to be developed in order to increase the performance of current antigen-based malaria rapid diagnosis. Antibody production often involves the use of laboratory animals and is time-consuming and costly, especially when the target is <jats:italic>Plasmodium<\/jats:italic>, whose variable\n antigen expression complicates the development of long-lived biomarkers. To circumvent these obstacles, we have applied the Systematic Evolution of Ligands by EXponential enrichment method to the rapid identification of DNA aptamers against <jats:italic>Plasmodium falciparum<\/jats:italic>-infected red blood cells\n (pRBCs). Five 70 b-long ssDNA sequences, and their shorter forms without the flanking PCR primer-binding regions, have been identified having a highly specific binding of pRBCs versus non-infected erythrocytes. Structural analysis revealed G-enriched sequences compatible with the formation\n of G-quadruplexes. The selected aptamers recognized intracellular epitopes with apparent <jats:italic>K<jats:sub>d<\/jats:sub><\/jats:italic>s in the <jats:italic>\u03bc<\/jats:italic>M range in both fixed and non-fixed saponin-permeabilized pRBCs, improving &gt;30-fold the pRBC detection in comparison with aptamers raised against <jats:italic>Plasmodium<\/jats:italic>\n lactate dehydrogenase, the gold standard antigen for current malaria diagnostic tests. In thin blood smears of clinical samples the aptamers reported in this work specifically bound all <jats:italic>P. falciparum<\/jats:italic> stages versus non-infected erythrocytes, and also detected early and late stages of\n the human malaria parasites <jats:italic>Plasmodium vivax<\/jats:italic>, <jats:italic>Plasmodium ovale<\/jats:italic> and <jats:italic>Plasmodium malariae<\/jats:italic>. The results are discussed in the context of their potential application in future malaria diagnostic devices.<\/jats:p>","DOI":"10.1166\/jbn.2020.2901","type":"journal-article","created":{"date-parts":[[2020,6,4]],"date-time":"2020-06-04T04:39:11Z","timestamp":1591245551000},"page":"315-334","source":"Crossref","is-referenced-by-count":8,"title":["Development of DNA Aptamers Against <i>Plasmodium falciparum<\/i> Blood Stages Using Cell-Systematic Evolution of Ligands by EXponential Enrichment"],"prefix":"10.1166","volume":"16","author":[{"given":"Elena","family":"Lantero","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandros","family":"Belavilas-Trovas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnau","family":"Biosca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Recolons","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernest","family":"Moles","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Sulleiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesc","family":"Zarzuela","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunuen","family":"\u00c1valos-Padilla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam","family":"Ram\u00edrez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Fern\u00e0ndez-Busquets","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"17","container-title":["Journal of Biomedical Nanotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ingentaconnect.com\/content\/asp\/jbn\/2020\/00000016\/00000003\/art00004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,6,6]],"date-time":"2020-06-06T04:37:39Z","timestamp":1591418259000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ingentaconnect.com\/content\/10.1166\/jbn.2020.2901"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,3,1]]}},"alternative-id":["1550-7033(20200301)16:3L.315;1-"],"URL":"https:\/\/doi.org\/10.1166\/jbn.2020.2901","relation":{},"ISSN":["1550-7033"],"issn-type":[{"value":"1550-7033","type":"print"}],"subject":[],"published":{"date-parts":[[2020,3,1]]}}}