{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T16:11:32Z","timestamp":1762272692223},"reference-count":79,"publisher":"American Scientific Publishers","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["j biomed nanotechnol"],"published-print":{"date-parts":[[2021,2,28]]},"abstract":"<jats:p>Current researches report an actual benefit of a treatment for oral cancer via inhibition of proteolytic matrix metallopro-teinases (MPP) with a peptide drug, called CTT1. However, peptides present poor oral bioavailability. Topical administration on oral mucosa avoids its passage through\n the gastrointestinal tract and the first-pass liver metabolism, but the barrier function of the oral mucosa can impair the permeation and retention of CTT1. The objective of this study is to incorporate CTT1 into a mucoadhesive precursor of liquid crystalline system (PLCS) as an interesting\n strategy for the topical treatment of oral cancer. PLCS consisting of oleic acid, ethoxylated 20 and propoxylated cetyl alcohol 5, polyethyleneimine (P)-associated chitosan (C) dispersion and CTT1 (FPC-CTT1) was developed and characterized by polarized light microscopy (PLM) and small-angle\n X-ray scattering (SAXS). <jats:italic>In vitro<\/jats:italic> permeation and retention across esophageal mucosa, <jats:italic>In vitro<\/jats:italic> cytotoxicity towards tongue squamous cell carcinoma cells, and <jats:italic>in vivo<\/jats:italic> evaluation of vascular changes using the chick embryo chorioallantoic membrane (CAM) model were performed.\n PLM and SAXS showed that FPC-CTT1acted as PLCS, because it formed a lamellar liquid crystalline system after the addition of artificial saliva. FPC-CTT1increased approximately 2-fold the flux of permeation and 3-fold the retention of CTT1 on the porcine esophageal mucosa. CTT1 does not affect\n cell viability. CAM tests showed that FPC preserved the blood vessels and it can be a safe formulation. These findings encourage the use of the FPC-CTT1 for topical treatment of oral cancer.<\/jats:p>","DOI":"10.1166\/jbn.2021.3025","type":"journal-article","created":{"date-parts":[[2021,3,31]],"date-time":"2021-03-31T09:40:35Z","timestamp":1617183635000},"page":"253-262","source":"Crossref","is-referenced-by-count":7,"title":["Innovative Mucoadhesive Precursor of Liquid Crystalline System Loading Anti-Gellatinolytic Peptide for Topical Treatment of Oral Cancer"],"prefix":"10.1166","volume":"17","author":[{"given":"Giovana Maria Fioramonti","family":"Calixto","sequence":"first","affiliation":[{"name":"UNICAMP, University of Campinas, Piracicaba Dental School Department of Biosciences, Piracicaba, SP, 13414-903, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesca Damiani","family":"Victorelli","sequence":"additional","affiliation":[{"name":"UNESP, S\u00e3o Paulo State University, School of Pharmaceutical Sciences, Department of Drugs and Medicines, Araraquara, SP, 14800-903, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle","family":"Franz-Montan","sequence":"additional","affiliation":[{"name":"UNICAMP, University of Campinas, Piracicaba Dental School Department of Biosciences, Piracicaba, SP, 13414-903, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F\u00e1tima","family":"Baltazar","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, 4710-057, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marlus","family":"Chorilli","sequence":"additional","affiliation":[{"name":"UNESP, S\u00e3o Paulo State University, School of Pharmaceutical Sciences, Department of Drugs and Medicines, Araraquara, SP, 14800-903, 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