{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T03:37:33Z","timestamp":1777347453602,"version":"3.51.4"},"reference-count":46,"publisher":"American Scientific Publishers","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["j biomed nanotechnol"],"published-print":{"date-parts":[[2021,2,28]]},"abstract":"<jats:p>Our previously prepared ternary nanocomposite TNT\/CuFe<jats:sub>2<\/jats:sub>O<jats:sub>4<\/jats:sub>\/Zn-Fe was highly engulfed by PC-3 cells, activated cytotoxicity that was dosage and time-subordinated, and demonstrated morphological alteration, which is one of the common characteristics of apoptotic cells.\n This prolonged study aimed to investigate other items. The study performed assays as Annexin V-FITC, flow cytometry, DNA ladder electrophoresis, and ROS assay for apoptosis detection, cell cycle analysis, DNA fragmentation, and ROS generation, respectively. In the PC-3-treated cells, the early\n and late phases of apoptosis with different percentages and DNA fragmentation were determined. Besides, the PC-3 cell cycle revealed the three major cell distribution different phases of the cycle (G1, S, and G2\/M), and the Sub G1, which corresponded to apoptotic cells. The results proved\n the presence of ROS that triggered the intrinsic apoptotic pathway, which was confirmed through a decrease in (Bcl-2), the release of cytochrome c, activation of caspase-9, and caspase-3. To conclude, the ternary nanocomposite TNT\/CuFe<jats:sub>2<\/jats:sub>O<jats:sub>4<\/jats:sub>\/Zn-Fe achieved biochemical features\n alterations and could induce intrinsic apoptosis of PC-3 cells. The planned work of the current research will illuminate the arrested phase in the cell cycle through studying tumor suppressor genes such as p53 and Retinoblastoma RB, <jats:italic>c-Myc oncogene,<\/jats:italic> and cyclin-dependent kinases (Cdks)\n as well as their regulators.<\/jats:p>","DOI":"10.1166\/jbn.2021.3027","type":"journal-article","created":{"date-parts":[[2021,3,31]],"date-time":"2021-03-31T09:40:35Z","timestamp":1617183635000},"page":"303-311","source":"Crossref","is-referenced-by-count":5,"title":["A Highly Cellular Uptake Ternary Nanocomposite Titanate Nano-Tubes\/CuFe<sub>2<\/sub>O<sub>4<\/sub>\/Zn\u2013Fe Could Induce Intrinsic Apoptosis of Prostate Cancer Cells: An Extended Study"],"prefix":"10.1166","volume":"17","author":[{"given":"Ahmed A. 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