{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T15:47:15Z","timestamp":1776440835664,"version":"3.51.2"},"reference-count":59,"publisher":"American Scientific Publishers","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["j biomed nanotechnol"],"published-print":{"date-parts":[[2021,4,1]]},"abstract":"<jats:p>The miR let-7b-5p (a kind of microRNAs) has many pathophysiological regulation effects, including human hepatocellular carcinoma (HCC) pathogenesis. This study investigated whether nanoparticle-mediated miR let-7b-5p could jointly enhance the therapeutic effect of sorafenib on HCC by\n inhibiting the proliferation of HCC cells, inducing apoptosis, and reversing drug resistance. We evaluated the level of miR let-7b-5p in sorafenib-resistant HepG2 cells (HepG2<jats:sup>R<\/jats:sup>) and HepG2 HCC cells by qRT-PCR and analyzed the biological effects of hepatocellular carcinoma treated\n with sorafenib with miR let-7b-5p, and further studied the toxicity of nanoparticles (Ab-miR-NPs) that deliver miR let-7b-5p mimics and target GPC3 on the surface of hepatocellular carcinoma cells. Results showed that, in HepG2 cells, the expression level of miR let-7b-5p was significantly\n higher than that in HepG2<jats:sup>R<\/jats:sup> cells. Targeted nanoparticle Ab-miR-NPs mediated the delivery of miR let-7b-5p to the HCC cytoplasm and released miRNA after being broken down, down-regulating the expression of IGF1R and inhibiting AKT\/mTOR and Ras\/Raf signal transmission. Ab-miR-NPs\n not only enhanced the proliferation of sorafenib in cultured HepG2<jats:sup>R<\/jats:sup> cells and induced cell apoptosis efficiency, but they also improved the anti-tumor activity in the mouse models. These results indicated that GPC3 antibody-modified PLGA-PLL (polylactic acid-glycolic acetic copolymer\n grafted hyper-branched polylysine) loaded miR let-7b-5p polymer nanoparticles combined with sorafenib may be a new treatment strategy for HCC resistant to sorafenib.<\/jats:p>","DOI":"10.1166\/jbn.2021.3033","type":"journal-article","created":{"date-parts":[[2022,1,21]],"date-time":"2022-01-21T04:38:08Z","timestamp":1642739888000},"page":"677-690","source":"Crossref","is-referenced-by-count":11,"title":["Targeted Delivery of Glypican 3 (GPC3) Antibody-Modified MicroRNA (miR let-7b-5p) Polymer Nanoparticles to Sorafenib-Resistant Hepatsocellular Carcinoma Cells"],"prefix":"10.1166","volume":"17","author":[{"given":"Shuping","family":"Zhou","sequence":"first","affiliation":[{"name":"Clinical Laboratory Medicine, First Affiliated Hospital, Anhui University ofScience & Technology, Huainan 232001, 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