{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T13:38:57Z","timestamp":1758893937828},"reference-count":41,"publisher":"American Scientific Publishers","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["j biomed nanotechnol"],"published-print":{"date-parts":[[2021,9,1]]},"abstract":"<jats:p>Dengue fever is a classic mosquito viral disease. Dengue virus non-structural protein-1 as a membrane-associated homologous dimer anchored to the surface of infected cells and also secreted into the blood. The nonstructural protein-1 levels are related to disease severity, and the presence of nonstructural protein-1 secreted from cells to the serum of people infected with the dengue virus is an early marker of infection. Paired antibodies are key in the establishment of rapid detection technology. In this study, the prepared recombinant nonstructural protein-1 protein of dengue virus serotype 3 was purified by the prokaryotic expression, and prepared monoclonal antibodies by cell fusion. A method for paired antibody screening was established based on the N-hydroxy succinimide-nanobeads and the prepared monoclonal antibodies. A simple and rapid point-of-care system integrating the paired antibodies and lateral flow assay was established to verify the screened antibody pairs. The results confirmed that the antibody pair screening method based on N-hydroxy succinimide-nanobeads is feasible.<\/jats:p>","DOI":"10.1166\/jbn.2021.3148","type":"journal-article","created":{"date-parts":[[2021,10,24]],"date-time":"2021-10-24T04:35:17Z","timestamp":1635050117000},"page":"1788-1797","source":"Crossref","is-referenced-by-count":1,"title":["Nanobead-Based Screening Method for Antibody Pairing of Dengue Virus Nonstructural Protein-1"],"prefix":"10.1166","volume":"17","author":[{"given":"Zhixin","family":"Chen","sequence":"first","affiliation":[{"name":"Research Center of Molecular Medicine of Yunnan Province, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuzhen","family":"He","sequence":"additional","affiliation":[{"name":"Research Center of Molecular Medicine of Yunnan Province, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruixian","family":"Xu","sequence":"additional","affiliation":[{"name":"Research Center of Molecular Medicine of Yunnan Province, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qinqin","family":"Han","sequence":"additional","affiliation":[{"name":"Research Center of Molecular Medicine of Yunnan Province, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueshan","family":"Xia","sequence":"additional","affiliation":[{"name":"Research Center of Molecular Medicine of Yunnan Province, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuzhu","family":"Song","sequence":"additional","affiliation":[{"name":"Research Center of Molecular Medicine of Yunnan Province, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinyang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Research Center of Molecular Medicine of Yunnan Province, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"17","reference":[{"issue":"(12 Suppl)","key":"r1","doi-asserted-by":"crossref","first-page":"pp.S98","DOI":"10.1038\/nm1144","article-title":"Emerging flaviviruses: The spread and resurgence of Japanese encephalitis, West Nile and dengue viruses","volume":"10","author":"Mackenzie","year":"2004","journal-title":"Nature Medicine"},{"key":"r2","year":"(2014)"},{"issue":"(16)","key":"r3","doi-asserted-by":"crossref","first-page":"pp.2773","DOI":"10.1007\/s00018-010-0357-z","article-title":"Dengue virus life cycle: Viral and host factors modulating infectivity","volume":"67","author":"Rodenhuis-Zybert","year":"2010","journal-title":"Cellular and Molecular Life Sciences"},{"issue":"(6157)","key":"r4","article-title":"Tropical medicine. 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