{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T16:29:39Z","timestamp":1748881779861},"reference-count":29,"publisher":"American Scientific Publishers","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["j biomed nanotechnol"],"published-print":{"date-parts":[[2022,3,1]]},"abstract":"<jats:p>In this manuscript we constructed a dual-responsive nano-drug delivery system for matrix metalloproteinases and ATP in ovarian cancer microenvironment. The nanomicelle PCL-DNA\/DOX-Peptide-PEG was prepared by intercalating doxorubicin hydrochloride between C and G base pairs of DNA double\n helix structure. Another ATP-responsive nanomicelle PCL-DNA\/DOX-PEG was prepared. Then we analyzed the characterization of nanomicelles (particle size, potential, surface morphology, etc.) and drug loading binding and drug release behavior. In addition, the effect of nanomicelles on the viability\n of mouse ovarian epithelial tumor cell ID-8 was detected by CCK-8 method. CCK-8 assay detected that different concentrations of carrier had no difference on the proliferation of ID-8 cells, and the survival rate of ID-8 cells by different concentrations of DOX preparations was statistically\n significant and the same results were observed in cytotoxicity comparison. Confocal microscopy showed that DOX in the drug-loaded micelle group was concentrated in the nucleus, while free DOX was concentrated in the cytoplasm. ID-8 cells took up the drug-loaded micelles faster. The semi-quantitative\n analysis of the DOX uptake of ID-8 cells with different treatments showed extremely significant statistical differences. In conclusion, the prepared self-assembled dual-responsive nanomicelle PCL-DNA\/DOX-Peptide-PEG is novel anti-tumor agent, and is expected to have good tumor tissue penetration\n ability with a low toxicity.<\/jats:p>","DOI":"10.1166\/jbn.2022.3303","type":"journal-article","created":{"date-parts":[[2022,6,18]],"date-time":"2022-06-18T04:42:13Z","timestamp":1655527333000},"page":"718-728","source":"Crossref","is-referenced-by-count":4,"title":["Construction of a New Dual-Responsive Nano-Drug Delivery System for Matrix Metalloproteinases and Adenosine Triphosphate in Ovarian Cancer Using Nanomicelles"],"prefix":"10.1166","volume":"18","author":[{"given":"Guocheng","family":"Li","sequence":"first","affiliation":[{"name":"Medical School, Southeast University, Nanjing, Jiangsu, 210000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenwen","family":"Xu","sequence":"additional","affiliation":[{"name":"Medical School, Southeast University, Nanjing, Jiangsu, 210000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Zhong Da Hospital, Southeast University, Nanjing, Jiangsu, 210000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengzhu","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Zhong Da Hospital, Southeast University, Nanjing, Jiangsu, 210000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danhong","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Zhong Da Hospital, Southeast University, Nanjing, Jiangsu, 210000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"17","reference":[{"key":"R104_473_437","first-page":"0","volume":"10","journal-title":"Ab"},{"key":"R105_125_454","first-page":"05","volume":"0","journal-title":"P <"},{"key":"R111_381_744","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.canlet.2015.07.039","volume":"368","journal-title":"Cancer Letters","ISSN":"http:\/\/id.crossref.org\/issn\/0096-3917","issn-type":"print"},{"key":"R111_378_651","doi-asserted-by":"crossref","first-page":"869","DOI":"10.1080\/17425247.2018.1517749","volume":"15","journal-title":"Expert Opinion on Drug Delivery","ISSN":"http:\/\/id.crossref.org\/issn\/1742-5247","issn-type":"print"},{"key":"R111_544_517","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1097\/01.gco.0000192996.20040.24","volume":"18","journal-title":"Current Opinion in Obstetrics and Gynecology","ISSN":"http:\/\/id.crossref.org\/issn\/1040-872X","issn-type":"print"},{"key":"R111_551_455","doi-asserted-by":"crossref","first-page":"191","DOI":"10.7314\/APJCP.2014.15.1.191","volume":"15","journal-title":"Asian Pacific Journal of Cancer Prevention","ISSN":"http:\/\/id.crossref.org\/issn\/1513-7368","issn-type":"print"},{"key":"R111_526_682","doi-asserted-by":"crossref","first-page":"1","DOI":"10.7150\/thno\/v01p0001","volume":"1","journal-title":"Theranostics"},{"key":"R111_534_609","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.jconrel.2020.04.026","volume":"323","journal-title":"Journal of Controlled Release","ISSN":"http:\/\/id.crossref.org\/issn\/0168-3659","issn-type":"print"},{"key":"R111_348_578","first-page":"4","volume":"24","journal-title":"Journal of Controlled Release","ISSN":"http:\/\/id.crossref.org\/issn\/0168-3659","issn-type":"print"},{"key":"R111_394_548","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.5604\/17322693.1184451","volume":"69","journal-title":"Postepy Hig. 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