{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T17:08:59Z","timestamp":1780506539934,"version":"3.54.1"},"reference-count":45,"publisher":"American Scientific Publishers","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["j biomed nanotechnol"],"published-print":{"date-parts":[[2022,4,1]]},"abstract":"<jats:p><jats:italic>Objective<\/jats:italic>: To evaluate the application of Ag\u2013Cu NPs as quorum sensing (QS) inhibitors and attenuate virulence expression to overcome the global crisis of multidrug-resistant (MDR) <jats:italic>P. aeruginosa<\/jats:italic>. <jats:italic>Methods<\/jats:italic>: Ag\u2013Cu NPs were synthesized by co-reduction of\n silver-nitrate and copper-nitrate (Ag:Cu = 1:1 0.75 <jats:italic>\u03bc<\/jats:italic>M). In this cross-sectional study, a total of eighty clinical strains of <jats:italic>P. aeruginosa<\/jats:italic> were collected from patients with burns. The antibacterial and resistance pattern of the clinical isolated was determined using the microdilution\n and Kirby Bauer disk methods. The effect of sub-MIC of Ag\u2013Cu NPs on the expression of lasI, exoS and toxA in five clinical isolates of imipenem-resistant <jats:italic>P. aeruginosa<\/jats:italic> was performed using qRT-PCR. <jats:italic>Results<\/jats:italic>: The characterization methods confirm the formation of the Ag\u2013Cu\n alloy NPs with agglomerated spherical morphology and particle sizes of about 30\u201340 nm. We observed that the MIC and MBC of Ag\u2013Cu alloy NPs against MDR <jats:italic>P. aeruginosa<\/jats:italic> was found to be 2.5 and 5 <jats:italic>\u03bc<\/jats:italic>g ml<jats:sup>\u22121<\/jats:sup>, respectively. The effects of a sub-inhibitory\n concentration of Ag\u2013Cu NPs on MDR <jats:italic>P. aeruginosa<\/jats:italic> QS and virulence-related genes showed that the expression level of QS regulatory and virulence genes significantly decreased in both MDR <jats:italic>P. aeruginosa<\/jats:italic> and reference strain under Ag\u2013Cu sub-MIC treatment. <jats:italic>Conclusion<\/jats:italic>:\n Ag\u2013Cu NPs could be potentially used as a promising QS inhibitor and anti-virulence compound against <jats:italic>P. aeruginosa<\/jats:italic>.<\/jats:p>","DOI":"10.1166\/jbn.2022.3331","type":"journal-article","created":{"date-parts":[[2022,7,20]],"date-time":"2022-07-20T00:42:42Z","timestamp":1658277762000},"page":"1196-1204","source":"Crossref","is-referenced-by-count":6,"title":["Antibacterial Properties of Ag\u2013Cu Alloy Nanoparticles Against Multidrug-Resistant <i>Pseudomonas aeruginosa<\/i> Through Inhibition of Quorum Sensing Pathway and Virulence-Related Genes"],"prefix":"10.1166","volume":"18","author":[{"given":"Mozhgan","family":"Pourjafari","sequence":"first","affiliation":[{"name":"Department of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, 009811, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masood","family":"Ghane","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Tonekabon Branch, Islamic Azad University, Tonekabon, 009811, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hami","family":"Kaboosi","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, 009811, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Babak","family":"Sadeghi","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Tonekabon Branch, Islamic Azad University, Tonekabon, 009811, 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