{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T00:47:57Z","timestamp":1648601277013},"reference-count":0,"publisher":"American Scientific Publishers","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["j nanosci nanotechnol"],"published-print":{"date-parts":[[2018,12,1]]},"abstract":"<jats:p>The exact mechanism of delayed death of <jats:italic>Toxoplasma gondii<\/jats:italic> is not known. FAS II synthesis in the apicoplast of <jats:italic>T. gondii<\/jats:italic> is essential for the survival of <jats:italic>Toxoplasma gondii<\/jats:italic>, while <jats:italic>\u03b2<\/jats:italic>-hydroxyacylacyl carrier protein dehydratase (FabZ) is indispensable for\n fatty acid synthesis. The present study investigated the relationship between the delayed death of <jats:italic>T. gondii<\/jats:italic> by inducing metabolic disorders due to suppression the expression of FabZ. A tetracycline-induced knockout vector inserted with <jats:italic>T. gondii fabZ<\/jats:italic> gene was constructed, and\n transfected into <jats:italic>T. gondii<\/jats:italic> TATi strain by electroporation. The stable mutants with tetracycline-induced knockout were selected, expression of FabZ was suppressed by using anhydrotetracycline (ATc), and FAS II deficient tachyzoites were prepared. The Western blot and qPCR results revealed\n that proliferation of FAS II deficient tachyzoites was not significantly different compared to the normal tachyzoites at 24 h and 48 h; however, after 72 h, the number of <jats:italic>T. gondii<\/jats:italic> tachyzoites in the ATc treated group was significantly (<jats:italic>p<\/jats:italic> &lt; 0.05) less than that of non-treated\n group, indicating the delayed death of <jats:italic>T. gondii<\/jats:italic> caused by the loss of apicoplast and decrease in the expression of FabZ, which inhibited the FAS II metabolism. The results of this study can be used for prevention of toxoplasmosis by inducing delayed death of <jats:italic>T. gondii.<\/jats:italic><\/jats:p>","DOI":"10.1166\/jnn.2018.16396","type":"journal-article","created":{"date-parts":[[2018,9,7]],"date-time":"2018-09-07T04:35:06Z","timestamp":1536294906000},"page":"8155-8159","source":"Crossref","is-referenced-by-count":2,"title":["Induction of FAS II Metabolic Disorders to Cause Delayed Death of <i>Toxoplasma gondii<\/i>"],"prefix":"10.1166","volume":"18","author":[{"given":"Liang","family":"Wu","sequence":"first","affiliation":[{"name":"School of Medicine, Jiangsu University, Zhenjiang 212013, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lipei","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The Affiliated Dongtai Hospital of Nantong University, Dongtai 224200, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenyu","family":"Tang","sequence":"additional","affiliation":[{"name":"Jingjiang College of Jiangsu University, Zhenjiang 212013, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiajian","family":"Wang","sequence":"additional","affiliation":[{"name":"Jingjiang College of Jiangsu University, Zhenjiang 212013, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoling","family":"Jin","sequence":"additional","affiliation":[{"name":"Jingjiang College of Jiangsu University, Zhenjiang 212013, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xugan","family":"Jiang","sequence":"additional","affiliation":[{"name":"School of Medicine, Jiangsu University, Zhenjiang 212013, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengxia","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Medicine, Jiangsu University, Zhenjiang 212013, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"17","container-title":["Journal of Nanoscience and Nanotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ingentaconnect.com\/content\/asp\/jnn\/2018\/00000018\/00000012\/art00017","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T04:33:13Z","timestamp":1578025993000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ingentaconnect.com\/content\/10.1166\/jnn.2018.16396"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,12,1]]},"references-count":0,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2018,12,1]]}},"alternative-id":["1533-4880(20181201)18:12L.8155;1-"],"URL":"https:\/\/doi.org\/10.1166\/jnn.2018.16396","relation":{},"ISSN":["1533-4880"],"issn-type":[{"value":"1533-4880","type":"print"}],"subject":[],"published":{"date-parts":[[2018,12,1]]}}}