{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T13:46:12Z","timestamp":1775051172335,"version":"3.50.1"},"reference-count":0,"publisher":"American Scientific Publishers","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["j nanosci nanotechnol"],"published-print":{"date-parts":[[2020,5,1]]},"abstract":"<jats:p>Dexmedetomidine (Dex) works as a crucial agent for the treatment of intestinal ischemia\/reperfusion (I\/R), but its mechanism remains unclear. Recent articles demonstrated the pivotal role of Janus kinase\/signal transducer and activator of transcription (JAK2\/STAT3) signalling in I\/R.\n Therefore, it is reasonable to explore the associated mechanism of JAK2\/STAT3 signalling in Dex treatment. The study purpose was to evaluate the JAK2\/STAT3 signalling regulatory mechanisms of Dex in preventing I\/R. Anaesthetized rats were subjected to superior mesenteric artery occlusion consisting\n of 1 h of ischemia and 2 h of reperfusion while served as controls. Animals received subcutaneous administration of 50 <jats:italic>\u03bc<\/jats:italic>g\/kg Dex, JAK1 and JAK2 inhibitor, Ruxolitinib, selective JAK2 inhibitor, 10 mg\/kg AG490 or STAT inhibitor and 0.4 mg\/kg rapamycin; or Dex-treatment in the presence\n of <jats:italic>\u03b1<\/jats:italic>2-adrenoceptor antagonists Atip or Dex-treatment alone after I\/R. Injury was scored histologically, apoptosis was detected via the apoptotic mediators caspase-3 and Bcl-2\/Bax and the degree of activation of the JAK\/STAT pathway was evaluated. Dex inhibited I\/R injury by decreasing\n apoptosis significantly with rescue of cleaved caspase-3 and the Bcl-2\/Bax ratio. Furthermore, phosphorylation of JAK2, STAT1 and STAT3 was affected, suggesting the involvement of activated JAK\/STAT in response to Dex. Meanwhile, the JAK2 or STAT inhibitors AG490 and rapamycin, but not Ruxolitinib,\n exhibited a similar but even greater JAK2 and STAT3 regulatory effect, thus leading to a greater benefit. JAK2\/STAT3 activation is crucial to the diminishing effect of Dex on mesenteric I\/R injury; however, the efficacy and timing of Dex administration should be considered in clinical practice.<\/jats:p>","DOI":"10.1166\/jnn.2020.16416","type":"journal-article","created":{"date-parts":[[2019,10,22]],"date-time":"2019-10-22T04:37:56Z","timestamp":1571719076000},"page":"3295-3302","source":"Crossref","is-referenced-by-count":24,"title":["The Role of Janus Kinase\/Signal Transducer and Activator of Transcription Signalling on Preventing Intestinal Ischemia\/Reperfusion Injury with Dexmedetomidine"],"prefix":"10.1166","volume":"20","author":[{"given":"Xuekang","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Anesthesiology, First Affiliated Hospital of Nanchang University, Nanchang 330006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Neurology, Shanghai Jiaotong University Affiliated Sixth People\u2019s Hospital South Campus, Shanghai 201499, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, First Affiliated Hospital of Nanchang University, Nanchang 330006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhengren","family":"Liu","sequence":"additional","affiliation":[{"name":"General Surgery, First Affiliated Hospital of Nanchang University, Nanchang 330006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiuhong","family":"Chen","sequence":"additional","affiliation":[{"name":"Grade 2015 of Medical Department of Graduate School, Nanchang University, Nanchang 330006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenxiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Grade 2015 of Medical Department of Graduate School, Nanchang University, Nanchang 330006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huaigen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, First Affiliated Hospital of Nanchang University, Nanchang 330006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, First Affiliated Hospital of Nanchang University, Nanchang 330006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanlu","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, First Affiliated Hospital of Nanchang University, Nanchang 330006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"17","container-title":["Journal of Nanoscience and Nanotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ingentaconnect.com\/content\/asp\/jnn\/2020\/00000020\/00000005\/art00080","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,11,28]],"date-time":"2019-11-28T04:32:55Z","timestamp":1574915575000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ingentaconnect.com\/content\/10.1166\/jnn.2020.16416"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,5,1]]}},"alternative-id":["1533-4880(20200501)20:5L.3295;1-"],"URL":"https:\/\/doi.org\/10.1166\/jnn.2020.16416","relation":{},"ISSN":["1533-4880"],"issn-type":[{"value":"1533-4880","type":"print"}],"subject":[],"published":{"date-parts":[[2020,5,1]]}}}