{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T21:57:01Z","timestamp":1791928621478,"version":"4.3.4"},"reference-count":33,"publisher":"Association for Research in Vision and Ophthalmology (ARVO)","issue":"3","license":[{"start":{"date-parts":[[2017,3,17]],"date-time":"2017-03-17T00:00:00Z","timestamp":1489708800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Invest. Ophthalmol. Vis. Sci."],"DOI":"10.1167\/iovs.16-20737","type":"journal-article","created":{"date-parts":[[2017,3,20]],"date-time":"2017-03-20T15:30:52Z","timestamp":1490023852000},"page":"1665","source":"Crossref","is-referenced-by-count":9,"title":["The Corneal Epithelial Barrier and Its Developmental Role in Isolating Corneal Epithelial and Conjunctival Cells From One Another"],"prefix":"10.1167","volume":"58","author":[{"given":"James K.","family":"Kubilus","sequence":"first","affiliation":[{"name":"Department of Integrative Physiology and Pathobiology, Tufts University School of Medicine, Boston, Massachusetts, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Zapater","sequence":"additional","affiliation":[{"name":"Department of Integrative Physiology and Pathobiology, Tufts University School of Medicine, Boston, Massachusetts, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas F.","family":"Linsenmayer","sequence":"additional","affiliation":[{"name":"Department of Integrative Physiology and Pathobiology, Tufts University School of Medicine, Boston, Massachusetts, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"27","published-online":{"date-parts":[[2017,3,17]]},"reference":[{"key":"i1552-5783-58-3-1665-b01","doi-asserted-by":"publisher","first-page":"424","DOI":"10.1006\/dbio.2000.9638","article-title":"The lens organizes the anterior segment: specification of neural crest cell differentiation in the avian eye","volume":"220","author":"Beebe","year":"2000","journal-title":"Dev Biol"},{"key":"i1552-5783-58-3-1665-b02","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1016\/j.ydbio.2011.04.008","article-title":"The mechanism of lens placode formation: a case of matrix-mediated morphogenesis","volume":"355","author":"Huang","year":"2011","journal-title":"Dev Biol"},{"key":"i1552-5783-58-3-1665-b03","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1016\/bs.pmbts.2015.04.003","article-title":"Corneal development: different cells from a common progenitor","volume":"134","author":"Lwigale","year":"2015","journal-title":"Prog Mol Biol Transl Sci"},{"key":"i1552-5783-58-3-1665-b04","doi-asserted-by":"publisher","first-page":"3383","DOI":"10.1242\/dev.125393","article-title":"Wnt\/beta-catenin signaling modulates corneal epithelium stratification via inhibition of Bmp4 during mouse development","volume":"142","author":"Zhang","year":"2015","journal-title":"Development"},{"key":"i1552-5783-58-3-1665-b05","doi-asserted-by":"publisher","first-page":"256","DOI":"10.1016\/j.ydbio.2015.09.009","article-title":"Negative and positive auto-regulation of BMP expression in early eye development","volume":"407","author":"Huang","year":"2015","journal-title":"Dev Biol"},{"key":"i1552-5783-58-3-1665-b06","doi-asserted-by":"publisher","first-page":"e0117089","DOI":"10.1371\/journal.pone.0117089","article-title":"Fibroblast growth factor receptor 2 (FGFR2) is required for corneal epithelial cell proliferation and differentiation during embryonic development","volume":"10","author":"Zhang","year":"2015","journal-title":"PLoS One"},{"key":"i1552-5783-58-3-1665-b07","doi-asserted-by":"publisher","first-page":"597","DOI":"10.1016\/bs.ctdb.2015.11.008","article-title":"Positional information and pattern formation","volume":"117","author":"Wolpert","year":"2016","journal-title":"Curr Top Dev Biol"},{"key":"i1552-5783-58-3-1665-b08","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1242\/dev.086900","article-title":"Bioelectric signaling regulates head and organ size during planarian regeneration","volume":"140","author":"Beane","year":"2013","journal-title":"Development"},{"key":"i1552-5783-58-3-1665-b09","doi-asserted-by":"publisher","first-page":"430","DOI":"10.1076\/ceyr.24.6.430.8599","article-title":"Changes in connexin43 in early ocular surface development","volume":"24","author":"Wolosin","year":"2002","journal-title":"Curr Eye Res"},{"key":"i1552-5783-58-3-1665-b10","doi-asserted-by":"publisher","first-page":"782","DOI":"10.1167\/iovs.09-3942","article-title":"Developmental corneal innervation: interactions between nerves and specialized apical corneal epithelial cells","volume":"51","author":"Kubilus","year":"2010","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"i1552-5783-58-3-1665-b11","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1002\/jmor.1050880104","article-title":"A series of normal stages in the development of the chick embryo","volume":"88","author":"Hamburger","year":"1951","journal-title":"J Morphol"},{"key":"i1552-5783-58-3-1665-b12","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1083\/jcb.96.1.124","article-title":"Monoclonal antibodies against chicken type V collagen: production, specificity, and use for immunocytochemical localization in embryonic cornea and other organs","volume":"96","author":"Linsenmayer","year":"1983","journal-title":"J Cell Biol"},{"key":"i1552-5783-58-3-1665-b13","doi-asserted-by":"publisher","first-page":"598","DOI":"10.1083\/jcb.100.2.598","article-title":"Immunohistochemical localization of short chain cartilage collagen (type X) in avian tissues","volume":"100","author":"Schmid","year":"1985","journal-title":"J Cell Biol"},{"key":"i1552-5783-58-3-1665-b14","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1046\/j.1432-0436.1997.6140251.x","article-title":"Stem cells of the corneal epithelium lack connexins and metabolite transfer capacity","volume":"61","author":"Matic","year":"1997","journal-title":"Differentiation"},{"key":"i1552-5783-58-3-1665-b15","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1006\/exer.1996.0175","article-title":"ZO1 in corneal epithelium: association to the zonula occludens and adherens junctions","volume":"64","author":"Sugrue","year":"1997","journal-title":"Exp Eye Res"},{"key":"i1552-5783-58-3-1665-b16","doi-asserted-by":"publisher","first-page":"663","DOI":"10.1016\/S0014-4835(03)00054-X","article-title":"Tight junction-related protein expression and distribution in human corneal epithelium","volume":"76","author":"Ban","year":"2003","journal-title":"Exp Eye Res"},{"key":"i1552-5783-58-3-1665-b17","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1007\/s11357-011-9359-5","article-title":"The impact of age on the physical and cellular properties of the human limbal stem cell niche","volume":"35","author":"Notara","year":"2013","journal-title":"Age (Dordr)"},{"key":"i1552-5783-58-3-1665-b18","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1016\/j.exer.2015.08.003","article-title":"Three-dimensional structure of the mammalian limbal stem cell niche","volume":"140","author":"Grieve","year":"2015","journal-title":"Exp Eye Res"},{"key":"i1552-5783-58-3-1665-b19","doi-asserted-by":"publisher","first-page":"1181","DOI":"10.1083\/jcb.121.5.1181","article-title":"Type V collagen: molecular structure and fibrillar organization of the chicken alpha 1(V) NH2-terminal domain, a putative regulator of corneal fibrillogenesis","volume":"121","author":"Linsenmayer","year":"1993","journal-title":"J Cell Biol"},{"key":"i1552-5783-58-3-1665-b20","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1083\/jcb.103.1.49","article-title":"Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells","volume":"103","author":"Schermer","year":"1986","journal-title":"J Cell Biol"},{"key":"i1552-5783-58-3-1665-b21","doi-asserted-by":"publisher","first-page":"765","DOI":"10.3109\/02713689409047012","article-title":"Conjunctival epithelial cells do not transdifferentiate in organotypic cultures: expression of K12 keratin is restricted to corneal epithelium","volume":"13","author":"Chen","year":"1994","journal-title":"Curr Eye Res"},{"key":"i1552-5783-58-3-1665-b22","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1089\/ten.tec.2010.0609","article-title":"A new isolation method of human limbal progenitor cells by maintaining close association with their niche cells","volume":"17","author":"Chen","year":"2011","journal-title":"Tissue Eng Part C Methods"},{"key":"i1552-5783-58-3-1665-b23","doi-asserted-by":"publisher","first-page":"172","DOI":"10.1016\/j.ydbio.2010.04.032","article-title":"Developmental guidance of embryonic corneal innervation: roles of Semaphorin3A and Slit2","volume":"344","author":"Kubilus","year":"2010","journal-title":"Dev Biol"},{"key":"i1552-5783-58-3-1665-b24","doi-asserted-by":"publisher","first-page":"750","DOI":"10.1016\/j.ydbio.2007.04.012","article-title":"Lens-derived Semaphorin3A regulates sensory innervation of the cornea","volume":"306","author":"Lwigale","year":"2007","journal-title":"Dev Biol"},{"key":"i1552-5783-58-3-1665-b25","doi-asserted-by":"publisher","first-page":"496","DOI":"10.1002\/dvdy.20621","article-title":"Expression patterns of Wnt genes during development of an anterior part of the chicken eye","volume":"235","author":"Fokina","year":"2006","journal-title":"Dev Dynamics"},{"key":"i1552-5783-58-3-1665-b26","doi-asserted-by":"publisher","first-page":"1642","DOI":"10.1002\/stem.1962","article-title":"microRNA-103\/107 family regulates multiple epithelial stem cell characteristics","volume":"33","author":"Peng","year":"2015","journal-title":"Stem Cells"},{"key":"i1552-5783-58-3-1665-b27","first-page":"1","article-title":"Fine structure of the developing avian cornea","volume":"1","author":"Hay","year":"1969","journal-title":"Monogr Dev Biol"},{"key":"i1552-5783-58-3-1665-b28","doi-asserted-by":"publisher","first-page":"C6","DOI":"10.1083\/jcb.31.1.C6","article-title":"Tight junctions between cells in the early chick embryo as visualized with the electron microscopy","volume":"31","author":"Trelstad","year":"1966","journal-title":"J Cell Biol"},{"key":"i1552-5783-58-3-1665-b29","doi-asserted-by":"publisher","first-page":"a002584","DOI":"10.1101\/cshperspect.a002584","article-title":"Physiology and function of the tight junction","volume":"1","author":"Anderson","year":"2009","journal-title":"Cold Spring Harb Perspect Biol"},{"issue":"(Suppl 2)","key":"i1552-5783-58-3-1665-b30","first-page":"21","article-title":"Epithelial transition zones: merging microenvironments, niches, and cellular transformation","volume":"21","author":"McNairn","year":"2011","journal-title":"Eur J Dermatol"},{"key":"i1552-5783-58-3-1665-b31","doi-asserted-by":"publisher","first-page":"1231","DOI":"10.1007\/s00384-010-0986-6","article-title":"New insights into the development and differentiation of the human anorectal epithelia","volume":"25","author":"Fritsch","year":"2010","journal-title":"Are there clinical consequences? Int J Colorectal Dis"},{"key":"i1552-5783-58-3-1665-b32","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1007\/s00795-011-0541-8","article-title":"Morphology of the epithelium of the lower rectum and the anal canal in the adult human","volume":"45","author":"Tanaka","year":"2012","journal-title":"Med Mol Morphol"},{"key":"i1552-5783-58-3-1665-b33","doi-asserted-by":"publisher","first-page":"G216","DOI":"10.1152\/ajpgi.00088.2015","article-title":"Esophageal development and epithelial homeostasis","volume":"309","author":"Rosekrans","year":"2015","journal-title":"Am J Physiol Gastrointest Liver Physiol"}],"container-title":["Investigative Ophthalmology & Visual Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/iovs.arvojournals.org\/article.aspx?articleid=2613126","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T21:30:37Z","timestamp":1791927037000},"score":1,"resource":{"primary":{"URL":"https:\/\/iovs.arvojournals.org\/article.aspx?articleid=2613126"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,3,17]]},"references-count":33,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,3,1]]}},"URL":"https:\/\/doi.org\/10.1167\/iovs.16-20737","relation":{},"ISSN":["1552-5783"],"issn-type":[{"value":"1552-5783","type":"print"}],"subject":[],"published":{"date-parts":[[2017,3,17]]}}}