{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T12:48:17Z","timestamp":1792673297720,"version":"4.5.0"},"reference-count":25,"publisher":"Association for Research in Vision and Ophthalmology (ARVO)","issue":"15","license":[{"start":{"date-parts":[[2023,12,20]],"date-time":"2023-12-20T00:00:00Z","timestamp":1703030400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Invest. Ophthalmol. Vis. Sci."],"published-print":{"date-parts":[[2023,12,20]]},"DOI":"10.1167\/iovs.64.15.25","type":"journal-article","created":{"date-parts":[[2023,12,20]],"date-time":"2023-12-20T10:30:57Z","timestamp":1703068257000},"page":"25","source":"Crossref","is-referenced-by-count":17,"title":["Mechanical Disruption of the Inner Limiting Membrane In Vivo Enhances Targeting to the Inner Retina"],"prefix":"10.1167","volume":"64","author":[{"given":"Jiun L.","family":"Do","sequence":"first","affiliation":[{"name":"Gleiberman Center for Glaucoma Research, Hamilton Glaucoma Center, Shiley Eye Institute and Viterbi Family Department of Ophthalmology, University of California San Diego, La Jolla, California, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Pedroarena-Leal","sequence":"additional","affiliation":[{"name":"Gleiberman Center for Glaucoma Research, Hamilton Glaucoma Center, Shiley Eye Institute and Viterbi Family Department of Ophthalmology, University of California San Diego, La Jolla, California, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikaela","family":"Louie","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Human Anatomy, University of California Davis, California, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Avila Garcia","sequence":"additional","affiliation":[{"name":"Gleiberman Center for Glaucoma Research, Hamilton Glaucoma Center, Shiley Eye Institute and Viterbi Family Department of Ophthalmology, University of California San Diego, La Jolla, California, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Alnihmy","sequence":"additional","affiliation":[{"name":"Gleiberman Center for Glaucoma Research, Hamilton Glaucoma Center, Shiley Eye Institute and Viterbi Family Department of Ophthalmology, University of California San Diego, La Jolla, California, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amit","family":"Patel","sequence":"additional","affiliation":[{"name":"Gleiberman Center for Glaucoma Research, Hamilton Glaucoma Center, Shiley Eye Institute and Viterbi Family Department of Ophthalmology, University of California San Diego, La Jolla, California, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert N.","family":"Weinreb","sequence":"additional","affiliation":[{"name":"Gleiberman Center for Glaucoma Research, Hamilton Glaucoma Center, Shiley Eye Institute and Viterbi Family Department of Ophthalmology, University of California San Diego, La Jolla, California, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karl J.","family":"Wahlin","sequence":"additional","affiliation":[{"name":"Gleiberman Center for Glaucoma Research, Hamilton Glaucoma Center, Shiley Eye Institute and Viterbi Family Department of Ophthalmology, University of California San Diego, La Jolla, California, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"La Torre Vila","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Human Anatomy, University of California Davis, California, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derek S.","family":"Welsbie","sequence":"additional","affiliation":[{"name":"Gleiberman Center for Glaucoma Research, Hamilton Glaucoma Center, Shiley Eye Institute and Viterbi Family Department of Ophthalmology, University of California San Diego, La Jolla, California, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"27","reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"4468","DOI":"10.1523\/JNEUROSCI.22-11-04468.2002","article-title":"Bcl-2 overexpression does not enhance in vivo axonal regeneration of retinal ganglion cells after peripheral nerve transplantation in adult mice","volume":"22","author":"Inoue","year":"2002","journal-title":"Journal of Neuroscience"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"1901","DOI":"10.1001\/jama.2014.3192","article-title":"The pathophysiology and treatment of glaucoma: a review","volume":"311","author":"Weinreb","year":"2014","journal-title":"JAMA"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"1972","DOI":"10.1002\/sctm.17-0059","article-title":"Enhanced stem cell differentiation and immunopurification of genome engineered human retinal ganglion cells","volume":"6","author":"Sluch","year":"2017","journal-title":"Stem Cells Transl Med"},{"key":"bib4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/srep16595","article-title":"Differentiation of human ESCs to retinal ganglion cells using a CRISPR engineered reporter cell line","volume":"5","author":"Sluch","year":"2015","journal-title":"Sci Rep"},{"key":"bib5","doi-asserted-by":"crossref","first-page":"3698","DOI":"10.1242\/dev.146290","article-title":"Three-dimensional automated reporter quantification (3D-ARQ) technology enables quantitative screening in retinal organoids","volume":"144","author":"Vergara","year":"2017","journal-title":"Development"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"14520","DOI":"10.1038\/s41598-018-32871-8","article-title":"Three-dimensional retinal organoids facilitate the investigation of retinal ganglion cell development, organization and neurite outgrowth from human pluripotent stem cells","volume":"8","author":"Fligor","year":"2018","journal-title":"Sci Rep"},{"key":"bib7","doi-asserted-by":"publisher","first-page":"10472","DOI":"10.1038\/ncomms10472","article-title":"Transplanted neurons integrate into adult retinas and respond to light","volume":"7","author":"Venugopalan","year":"2016","journal-title":"Nat Commun"},{"key":"bib8","first-page":"26","article-title":"Transplanted mouse embryonic stem cell\u2013derived retinal ganglion cells integrate and form synapses in a retinal ganglion cell-depleted mouse model","volume":"62","author":"Wu","year":"2021","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"bib9","doi-asserted-by":"publisher","first-page":"855","DOI":"10.3727\/096368913X667024","article-title":"Survival and integration of developing and progenitor-derived retinal ganglion cells following transplantation","volume":"23","author":"Hertz","year":"2014","journal-title":"Cell Transplant"},{"key":"bib10","doi-asserted-by":"publisher","first-page":"180","DOI":"10.1016\/j.omtm.2021.03.004","article-title":"Transplantation of miPSC\/mESC-derived retinal ganglion cells into healthy and glaucomatous retinas","volume":"21","author":"Oswald","year":"2021","journal-title":"Mol Ther Methods Clin Dev"},{"key":"bib11","doi-asserted-by":"publisher","first-page":"2096","DOI":"10.1038\/mt.2009.181","article-title":"Inner limiting membrane barriers to AAV-mediated retinal transduction from the vitreous","volume":"17","author":"Dalkara","year":"2009","journal-title":"Mol Ther"},{"key":"bib12","doi-asserted-by":"publisher","first-page":"355","DOI":"10.1007\/BF02150029","article-title":"Structure and composition of the inner limiting membrane of the retina. SEM on frozen resin-cracked and enzyme-digested retinas of Macaca mulatta","volume":"224","author":"Heegaard","year":"1986","journal-title":"Graefes Arch Clin Exp Ophthalmol"},{"key":"bib13","doi-asserted-by":"publisher","first-page":"3693","DOI":"10.1021\/pr5002065","article-title":"Proteomic view of basement membranes from human retinal blood vessels, inner limiting membranes, and lens capsules","volume":"13","author":"Uechi","year":"2014","journal-title":"J Proteome Res"},{"key":"bib14","doi-asserted-by":"publisher","first-page":"1207","DOI":"10.1038\/eye.2008.19","article-title":"Origin and turnover of ECM proteins from the inner limiting membrane and vitreous body","volume":"22","author":"Halfter","year":"2008","journal-title":"Eye (Lond)"},{"key":"bib16","doi-asserted-by":"publisher","first-page":"108582","DOI":"10.1016\/j.exer.2021.108582","article-title":"Importance of the inner limiting membrane in adults","volume":"207","author":"Gui","year":"2021","journal-title":"Exp Eye Res"},{"key":"bib17","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1016\/j.stemcr.2020.12.001","article-title":"Role of the internal limiting membrane in structural engraftment and topographic spacing of transplanted human stem cell-derived retinal ganglion cells","volume":"16","author":"Zhang","year":"2021","journal-title":"Stem Cell Reports"},{"key":"bib18","first-page":"97","article-title":"Optimal management of idiopathic macular holes","volume":"10","author":"Madi","year":"2016","journal-title":"Clin Ophthalmol"},{"key":"bib19","doi-asserted-by":"publisher","first-page":"500","DOI":"10.1186\/s13063-020-04433-9","article-title":"Does internal limiting membrane peeling during epiretinal membrane surgery induce microscotomas on microperimetry? Study protocol for PEELING, a randomized controlled clinical trial","volume":"21","author":"Ducloyer","year":"2020","journal-title":"Trials"},{"key":"bib20","doi-asserted-by":"publisher","first-page":"3503","DOI":"10.1167\/iovs.07-1601","article-title":"Development and characterization of an adult retinal explant organotypic tissue culture system as an in vitro intraocular stem cell transplantation model","volume":"49","author":"Johnson","year":"2008","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"bib21","first-page":"1","article-title":"Organotypic explant culture of adult rat retina for in vitro investigations of neurodegeneration, neuroprotection and cell transplantation","author":"Johnson","year":"2011","journal-title":"Protoc Exch"},{"key":"bib22","doi-asserted-by":"publisher","first-page":"33597","DOI":"10.1073\/pnas.2004683117","article-title":"Inhibition of GCK-IV kinases dissociates cell death and axon regeneration in CNS neurons","volume":"117","author":"Patel","year":"2020","journal-title":"Proc Natl Acad Sci USA"},{"key":"bib24","first-page":"465","article-title":"Chemical vitrectomy: change of solid gel vitreous to sol for vitrectomy by changing pH of vitreous toward the slight acid side","volume":"14","author":"Mortada","year":"1982","journal-title":"Ann Ophthalmol"},{"key":"bib25","doi-asserted-by":"publisher","first-page":"1098","DOI":"10.3109\/02713683.2015.1085578","article-title":"Vitreomacular attachment ultrastructure and histopathological correlation","volume":"41","author":"Breazzano","year":"2016","journal-title":"Curr Eye Res"},{"key":"bib15","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1007\/978-1-4939-1086-1_11","article-title":"Vitreoretinal interface and inner limiting membrane","volume-title":"Vitreous","author":"Halfter","year":"2014"},{"key":"bib23","doi-asserted-by":"crossref","DOI":"10.1007\/978-1-62703-128-8_1","article-title":"Detection of Mycoplasma Contaminations","volume-title":"Basic Cell Culture Protocols","author":"Uphoff","year":"2013"}],"container-title":["Investigative Opthalmology & Visual Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/iovs.arvojournals.org\/article.aspx?articleid=2793206","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T21:31:41Z","timestamp":1791927101000},"score":1,"resource":{"primary":{"URL":"https:\/\/iovs.arvojournals.org\/article.aspx?articleid=2793206"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,20]]},"references-count":25,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2023,12,1]]}},"URL":"https:\/\/doi.org\/10.1167\/iovs.64.15.25","relation":{},"ISSN":["1552-5783"],"issn-type":[{"value":"1552-5783","type":"print"}],"subject":[],"published":{"date-parts":[[2023,12,20]]}}}