{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T22:01:31Z","timestamp":1791928891110,"version":"4.3.4"},"reference-count":36,"publisher":"Association for Research in Vision and Ophthalmology (ARVO)","issue":"6","license":[{"start":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T00:00:00Z","timestamp":1750636800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Invest. Ophthalmol. Vis. Sci."],"published-print":{"date-parts":[[2025,6,23]]},"DOI":"10.1167\/iovs.66.6.69","type":"journal-article","created":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T08:30:30Z","timestamp":1750667430000},"page":"69","source":"Crossref","is-referenced-by-count":0,"title":["Small-Molecule Inhibition of the Hippo Pathway Induces Regeneration of Retinal Pigment Epithelium"],"prefix":"10.1167","volume":"66","author":[{"given":"Erik A.","family":"Souverein","sequence":"first","affiliation":[{"name":"Keck School of Medicine of USC, University of Southern California, Los Angeles, California, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanyukta","family":"Oak","sequence":"additional","affiliation":[{"name":"USC Caruso Department of Otolaryngology \u2013 Head and Neck Surgery, Keck School of Medicine of USC, University of Southern California, Los Angeles, California, United States"},{"name":"Stem Cell Biology and Regenerative Medicine, Keck School of Medicine of USC, University of Southern California, Los Angeles, California, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zachary","family":"Fouladian","sequence":"additional","affiliation":[{"name":"Keck School of Medicine of USC, University of Southern California, Los Angeles, California, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark W.","family":"Reid","sequence":"additional","affiliation":[{"name":"The Vision Center, Department of Surgery, Children's Hospital Los Angeles, Los Angeles, California, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. Esteban","family":"Fernandez","sequence":"additional","affiliation":[{"name":"The Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, California, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Salas","sequence":"additional","affiliation":[{"name":"The Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, California, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer G.","family":"Aparicio","sequence":"additional","affiliation":[{"name":"The Vision Center, Department of Surgery, Children's Hospital Los Angeles, Los Angeles, California, United States"},{"name":"The Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, California, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron","family":"Nagiel","sequence":"additional","affiliation":[{"name":"The Vision Center, Department of Surgery, Children's Hospital Los Angeles, Los Angeles, California, United States"},{"name":"The Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, California, United States"},{"name":"USC Roski Eye Institute, Keck School of Medicine of USC, University of Southern California, Los Angeles, California, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ksenia","family":"Gnedeva","sequence":"additional","affiliation":[{"name":"USC Caruso Department of Otolaryngology \u2013 Head and Neck Surgery, Keck School of Medicine of USC, University of Southern California, Los Angeles, California, United States"},{"name":"Stem Cell Biology and Regenerative Medicine, Keck School of Medicine of USC, University of Southern California, Los Angeles, California, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"27","reference":[{"issue":"1","key":"bib1","doi-asserted-by":"publisher","first-page":"e1007939","DOI":"10.1371\/journal.pgen.1007939","article-title":"Regeneration of the zebrafish retinal pigment epithelium after widespread genetic ablation","volume":"15","author":"Hanovice","year":"2019","journal-title":"PLoS Genet"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1186\/1741-7007-12-28","article-title":"Reprogramming of the chick retinal pigmented epithelium after retinal injury","volume":"12","author":"Luz-Madrigal","year":"2014","journal-title":"BMC Biol"},{"issue":"8","key":"bib3","doi-asserted-by":"publisher","first-page":"1333","DOI":"10.1002\/glia.23165","article-title":"M\u00fcller glial cell reactivation in Xenopus models of retinal degeneration","volume":"65","author":"Langhe","year":"2017","journal-title":"Glia"},{"issue":"7","key":"bib4","doi-asserted-by":"publisher","first-page":"727","DOI":"10.1002\/dvdy.24375","article-title":"M\u00fcller glial cell-dependent regeneration of the neural retina: an overview across vertebrate model systems","volume":"245","author":"Hamon","year":"2016","journal-title":"Dev Dyn"},{"issue":"2","key":"bib5","doi-asserted-by":"publisher","first-page":"438","DOI":"10.3390\/cells9020438","article-title":"Single-cell RNA sequencing in human retinal degeneration reveals distinct glial cell populations","volume":"9","author":"Voigt","year":"2020","journal-title":"Cells"},{"issue":"3","key":"bib6","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1080\/02713683.2019.1669665","article-title":"Rebuilding the retina: prospects for M\u00fcller glial-mediated self-repair","volume":"45","author":"Langhe","year":"2020","journal-title":"Curr Eye Res"},{"issue":"5","key":"bib7","doi-asserted-by":"publisher","first-page":"946","DOI":"10.1038\/s41433-018-0061-z","article-title":"Cellular regeneration strategies for macular degeneration: past, present and future","volume":"32","author":"Chichagova","year":"2018","journal-title":"Eye (Lond)"},{"issue":"6","key":"bib8","doi-asserted-by":"publisher","first-page":"1712","DOI":"10.1016\/j.celrep.2019.04.045","article-title":"Linking YAP to M\u00fcller glia quiescence exit in the degenerative retina","volume":"27","author":"Hamon","year":"2019","journal-title":"Cell Rep"},{"issue":"6","key":"bib9","doi-asserted-by":"publisher","first-page":"1637","DOI":"10.1016\/j.celrep.2019.04.047","article-title":"The Hippo pathway blocks mammalian retinal M\u00fcller glial cell reprogramming","volume":"27","author":"Rueda","year":"2019","journal-title":"Cell Rep"},{"issue":"3","key":"bib10","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1016\/j.devcel.2019.06.003","article-title":"The Hippo signaling pathway in development and disease","volume":"50","author":"Zheng","year":"2019","journal-title":"Dev Cell"},{"issue":"2","key":"bib11","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1007\/s00018-020-03579-8","article-title":"An overview of signaling pathways regulating YAP\/TAZ activity","volume":"78","author":"Heng","year":"2021","journal-title":"Cell Mol Life Sci"},{"issue":"5","key":"bib12","doi-asserted-by":"publisher","first-page":"6757","DOI":"10.1096\/fj.201903234R","article-title":"Yap1 is required for maintenance of adult RPE differentiation","volume":"34","author":"Lu","year":"2020","journal-title":"FASEB J"},{"issue":"1","key":"bib13","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.devcel.2017.11.011","article-title":"Differential expression of NF2 in neuroepithelial compartments is necessary for mammalian eye development","volume":"44","author":"Moon","year":"2018","journal-title":"Dev Cell"},{"issue":"1","key":"bib14","doi-asserted-by":"publisher","first-page":"3100","DOI":"10.1038\/s41467-021-23395-3","article-title":"Small-molecule inhibition of Lats kinases may promote Yap-dependent proliferation in postmitotic mammalian tissues","volume":"12","author":"Kastan","year":"2021","journal-title":"Nat Commun"},{"issue":"28","key":"bib15","doi-asserted-by":"publisher","first-page":"e2206113119","DOI":"10.1073\/pnas.2206113119","article-title":"Development of an improved inhibitor of Lats kinases to promote regeneration of mammalian organs","volume":"119","author":"Kastan","year":"2022","journal-title":"Proc Natl Acad Sci USA"},{"issue":"8","key":"bib16","doi-asserted-by":"publisher","first-page":"3612","DOI":"10.1167\/iovs.05-1622","article-title":"Confluent monolayers of cultured human fetal retinal pigment epithelium exhibit morphology and physiology of native tissue","volume":"47","author":"Maminishkis","year":"2006","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"10","key":"bib17","doi-asserted-by":"publisher","first-page":"2427","DOI":"10.1002\/stem.189","article-title":"Derivation of functional retinal pigmented epithelium from induced pluripotent stem cells","volume":"27","author":"Buchholz","year":"2009","journal-title":"Stem Cells"},{"key":"bib18","first-page":"56274","article-title":"Rapid, directed differentiation of retinal pigment epithelial cells from human embryonic or induced pluripotent stem cells","volume":"128","author":"Foltz","year":"2017","journal-title":"J Vis Exp"},{"issue":"4","key":"bib19","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1089\/jop.2019.0116","article-title":"A semiautomated, phenotypic, in vitro scratch assay for assessing retinal pigment epithelial cell wound healing","volume":"36","author":"Storm","year":"2020","journal-title":"J Ocul Pharmacol Ther"},{"issue":"1","key":"bib20","doi-asserted-by":"publisher","first-page":"16878","DOI":"10.1038\/s41598-017-17204-5","article-title":"QuPath: open source software for digital pathology image analysis","volume":"7","author":"Bankhead","year":"2017","journal-title":"Sci Rep."},{"issue":"12","key":"bib21","doi-asserted-by":"publisher","first-page":"5540","DOI":"10.1167\/iovs.08-1928","article-title":"Healing of retinal photocoagulation lesions","volume":"49","author":"Paulus","year":"2008","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"bib22","first-page":"e66115","article-title":"Obtaining high-quality cryosections of whole rabbit eye","volume":"201","author":"Souverein","year":"2023","journal-title":"J Vis Exp"},{"issue":"7","key":"bib23","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat Methods"},{"key":"bib24","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1016\/S0074-7696(03)30005-1","article-title":"Up-regulation of glial fibrillary acidic protein in response to retinal injury: its potential role in glial remodeling and a comparison to vimentin expression","volume":"230","author":"Lewis","year":"2003","journal-title":"Int Rev Cytol"},{"key":"bib25","doi-asserted-by":"publisher","first-page":"303","DOI":"10.1007\/978-3-319-75402-4","article-title":"M\u00fcller glia reactivity and development of gliosis in response to pathological conditions","volume":"1074","author":"Graca","year":"2018","journal-title":"Adv Exp Med Biol"},{"issue":"12","key":"bib26","doi-asserted-by":"publisher","first-page":"1202","DOI":"10.1001\/jamaophthalmol.2022.4401","article-title":"Prevalence of age-related macular degeneration in the US in 2019","volume":"140","author":"Rein","year":"2022","journal-title":"JAMA Ophthalmol"},{"issue":"10411","key":"bib27","doi-asserted-by":"publisher","first-page":"1434","DOI":"10.1016\/S0140-6736(23)01520-9","article-title":"Pegcetacoplan for the treatment of geographic atrophy secondary to age-related macular degeneration (OAKS and DERBY): two multicentre, randomised, double-masked, sham-controlled, phase 3 trials","volume":"402","author":"Heier","year":"2023","journal-title":"Lancet"},{"issue":"9967","key":"bib28","doi-asserted-by":"publisher","first-page":"509","DOI":"10.1016\/S0140-6736(14)61376-3","article-title":"Human embryonic stem cell-derived retinal pigment epithelium in patients with age-related macular degeneration and Stargardt's macular dystrophy: follow-up of two open-label phase 1\/2 studies","volume":"385","author":"Schwartz","year":"2015","journal-title":"Lancet"},{"key":"bib29","doi-asserted-by":"publisher","first-page":"621007","DOI":"10.3389\/fimmu.2021.621007","article-title":"Immunologic rejection of transplanted retinal pigmented epithelium: mechanisms and strategies for prevention","volume":"12","author":"Petrash","year":"2021","journal-title":"Front Immunol"},{"key":"bib30","doi-asserted-by":"publisher","first-page":"100779","DOI":"10.1016\/j.preteyeres.2019.100779","article-title":"Retinal stem cell transplantation: balancing safety and potential","volume":"75","author":"Singh","year":"2020","journal-title":"Prog Retin Eye Res"},{"issue":"9","key":"bib31","doi-asserted-by":"publisher","first-page":"7018","DOI":"10.1074\/jbc.C110.212621","article-title":"Hippo pathway-independent restriction of TAZ and YAP by angiomotin","volume":"286","author":"Chan","year":"2011","journal-title":"J Biol Chem"},{"issue":"24","key":"bib32","doi-asserted-by":"publisher","first-page":"12509","DOI":"10.18632\/oncotarget.2997","article-title":"Hypoxia-inducible factor 1 mediates TAZ expression and nuclear localization to induce the breast cancer stem cell phenotype","volume":"5","author":"Xiang","year":"2014","journal-title":"Oncotarget"},{"issue":"14","key":"bib33","doi-asserted-by":"publisher","first-page":"e2411313122","DOI":"10.1073\/pnas.2411313122","article-title":"The Hippo pathway and p27Kip1 cooperate to suppress mitotic regeneration in the organ of Corti and the retina","volume":"122","author":"Jahanshir","year":"2025","journal-title":"Proc Natl Acad Sci USA"},{"issue":"6","key":"bib34","doi-asserted-by":"publisher","first-page":"263","DOI":"10.21037\/atm.2020.03.86","article-title":"RPE epithelial\u2013mesenchymal transition plays a critical role in the pathogenesis of proliferative vitreoretinopathy","volume":"8","author":"Li","year":"2020","journal-title":"Ann Transl Med"},{"issue":"9","key":"bib35","doi-asserted-by":"publisher","first-page":"3441","DOI":"10.1172\/JCI93825","article-title":"YAP\/TAZ regulates sprouting angiogenesis and vascular barrier maturation","volume":"127","author":"Kim","year":"2017","journal-title":"J Clin Invest"},{"issue":"10","key":"bib36","doi-asserted-by":"publisher","first-page":"3200","DOI":"10.1016\/j.celrep.2019.03.021","article-title":"YAP\/TAZ activation drives uveal melanoma initiation and progression","volume":"29","author":"Li","year":"2019","journal-title":"Cell Rep"}],"container-title":["Investigative Ophthalmology & Visual Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/iovs.arvojournals.org\/article.aspx?articleid=2803163","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T21:32:11Z","timestamp":1791927131000},"score":1,"resource":{"primary":{"URL":"https:\/\/iovs.arvojournals.org\/article.aspx?articleid=2803163"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,23]]},"references-count":36,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,6,2]]}},"URL":"https:\/\/doi.org\/10.1167\/iovs.66.6.69","relation":{},"ISSN":["1552-5783"],"issn-type":[{"value":"1552-5783","type":"print"}],"subject":[],"published":{"date-parts":[[2025,6,23]]}}}