{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T22:02:33Z","timestamp":1791928953426,"version":"4.3.4"},"reference-count":59,"publisher":"Association for Research in Vision and Ophthalmology (ARVO)","issue":"12","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Invest. Ophthalmol. Vis. Sci."],"published-print":{"date-parts":[[2026,10,6]]},"DOI":"10.1167\/iovs.67.12.11","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:30:38Z","timestamp":1791293438000},"page":"11","source":"Crossref","is-referenced-by-count":0,"title":["Regeneration and Cancer\u2014The Two Sides of a \u201cStem Cell\u201d Coin: The 2025 Mildred Weisenfeld Award Lecture"],"prefix":"10.1167","volume":"67","author":[{"given":"Geeta K.","family":"Vemuganti","sequence":"first","affiliation":[{"name":"School of Medical Sciences, L. V. Prasad Marg, Banjara Hills, Hyderabad, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"27","reference":[{"key":"bib2","doi-asserted-by":"publisher","first-page":"326","DOI":"10.1038\/nature10147","article-title":"Heart regeneration","volume":"473","author":"Laflamme","year":"2011","journal-title":"Nature"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1172\/JCI40373","article-title":"Repairing skeletal muscle: regenerative potential of skeletal muscle stem cells","volume":"120","author":"Tedesco","year":"2010","journal-title":"J Clin Invest"},{"key":"bib4","doi-asserted-by":"publisher","first-page":"926059","DOI":"10.1155\/2012\/926059","article-title":"Stem cell niches for skin regeneration","volume":"2012","author":"Wong","year":"2012","journal-title":"Int J Biomater"},{"key":"bib5","doi-asserted-by":"publisher","first-page":"e1705388","DOI":"10.1002\/adma.201705388","article-title":"Looking into the future: toward advanced 3D biomaterials for stem-cell-based regenerative medicine","volume":"30","author":"Liu","year":"2018","journal-title":"Adv Mater"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1056\/NEJM200007133430202","article-title":"Reconstruction of damaged corneas by transplantation of autologous limbal epithelial cells","volume":"343","author":"Tsai","year":"2000","journal-title":"N Engl J Med"},{"key":"bib7","doi-asserted-by":"publisher","first-page":"868","DOI":"10.1097\/00007890-199909270-00021","article-title":"The control of epidermal stem cells (holoclones) in the treatment of massive full-thickness burns with autologous keratinocytes cultured on fibrin","volume":"68","author":"Pellegrini","year":"1999","journal-title":"Transplantation"},{"key":"bib8","doi-asserted-by":"publisher","first-page":"2584","DOI":"10.2353\/ajpath.2010.091064","article-title":"On the stem cell origin of cancer","volume":"176","author":"Sell","year":"2010","journal-title":"Am J Pathol"},{"key":"bib9","doi-asserted-by":"publisher","first-page":"481","DOI":"10.1023\/A:1017995810755","article-title":"Amniotic membrane transplantation for ocular surface reconstruction","volume":"21","author":"Tseng","year":"2001","journal-title":"Biosci Rep"},{"key":"bib10","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1056\/NEJMoa0905955","article-title":"Limbal stem-cell therapy and long-term corneal regeneration","volume":"363","author":"Rama","year":"2010","journal-title":"N Engl J Med"},{"key":"bib11","doi-asserted-by":"publisher","first-page":"1291","DOI":"10.1097\/ICO.0000000000002358","article-title":"Global consensus on the management of limbal stem cell deficiency","volume":"39","author":"Deng","year":"2020","journal-title":"Cornea"},{"key":"bib12","first-page":"257","article-title":"Technique of cultivating limbal derived corneal epithelium on human amniotic membrane for clinical transplantation","volume":"52","author":"Fatima","year":"2006","journal-title":"J Postgrad Med"},{"key":"bib13","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1002\/term.91","article-title":"A biomimetic scaffold for culturing limbal stem cells: a promising alternative for clinical transplantation","volume":"2","author":"Dravida","year":"2008","journal-title":"J Tissue Eng Regen Med"},{"key":"bib14","first-page":"113","article-title":"Ex-vivo potential of cadaveric and fresh limbal tissues to regenerate cultured epithelium","volume":"52","author":"Vemuganti","year":"2004","journal-title":"Indian J Ophthalmol"},{"key":"bib15","doi-asserted-by":"publisher","first-page":"355","DOI":"10.1634\/stemcells.22-3-355","article-title":"Characterization of putative stem cell phenotype in human limbal epithelia","volume":"22","author":"Chen","year":"2004","journal-title":"Stem Cells"},{"key":"bib16","doi-asserted-by":"publisher","first-page":"1470","DOI":"10.1038\/nprot.2010.115","article-title":"In vitro culture and expansion of human limbal epithelial cells","volume":"5","author":"Mariappan","year":"2010","journal-title":"Nat Protoc"},{"key":"bib17","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1111\/j.1442-9071.2007.01358.x","article-title":"In vivo survival and stratification of cultured limbal epithelium","volume":"35","author":"Fatima","year":"2007","journal-title":"Clin Exp Ophthalmol"},{"key":"bib18","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1097\/ICO.0b013e3181cbf9d3","article-title":"Vernal keratoconjunctivitis with limbal stem cell deficiency","volume":"30","author":"Sangwan","year":"2011","journal-title":"Cornea"},{"key":"bib19","doi-asserted-by":"publisher","first-page":"478","DOI":"10.1097\/00003226-200307000-00016","article-title":"Use of autologous cultured limbal and conjunctival epithelium in a patient with severe bilateral ocular surface disease induced by acid injury: a case report of unique application","volume":"22","author":"Sangwan","year":"2003","journal-title":"Cornea"},{"key":"bib20","doi-asserted-by":"publisher","first-page":"426","DOI":"10.1097\/01.ico.0000151508.49565.8a","article-title":"Cultivated corneal epithelial transplantation for severe ocular surface disease in vernal keratoconjunctivitis","volume":"24","author":"Sangwan","year":"2005","journal-title":"Cornea"},{"key":"bib21","doi-asserted-by":"publisher","first-page":"1504","DOI":"10.1136\/bjophthalmol-2012-301869","article-title":"Clinical outcomes of xeno-free allogeneic cultivated limbal epithelial transplantation for bilateral limbal stem cell deficiency","volume":"96","author":"Basu","year":"2012","journal-title":"Br J Ophthalmol"},{"key":"bib22","doi-asserted-by":"publisher","first-page":"29","DOI":"10.4103\/0301-4738.21611","article-title":"Clinical outcome of autologous cultivated limbal epithelium transplantation","volume":"54","author":"Sangwan","year":"2006","journal-title":"Indian J Ophthalmol"},{"key":"bib23","doi-asserted-by":"publisher","first-page":"1525","DOI":"10.1136\/bjophthalmol-2011-300352","article-title":"Clinical outcomes of xeno-free autologous cultivated limbal epithelial transplantation: a 10-year study","volume":"95","author":"Sangwan","year":"2011","journal-title":"Br J Ophthalmol"},{"key":"bib24","doi-asserted-by":"publisher","first-page":"334","DOI":"10.1001\/archopht.123.3.334","article-title":"Early results of penetrating keratoplasty after cultivated limbal epithelium transplantation","volume":"123","author":"Sangwan","year":"2005","journal-title":"Arch Ophthalmol"},{"key":"bib25","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1023\/B:BIRE.0000007690.43273.73","article-title":"Successful reconstruction of damaged ocular outer surface in humans using limbal and conjuctival stem cell culture methods","volume":"23","author":"Sangwan","year":"2003","journal-title":"Biosci Rep"},{"key":"bib26","doi-asserted-by":"publisher","first-page":"931","DOI":"10.1136\/bjophthalmol-2011-301164","article-title":"Simple limbal epithelial transplantation (SLET): a novel surgical technique for the treatment of unilateral limbal stem cell deficiency","volume":"96","author":"Sangwan","year":"2012","journal-title":"Br J Ophthalmol"},{"key":"bib27","doi-asserted-by":"publisher","first-page":"731","DOI":"10.1001\/jamaophthalmol.2013.2308","article-title":"Cultivated limbal epithelial transplantation in children with ocular surface burns","volume":"131","author":"Sejpal","year":"2013","journal-title":"JAMA Ophthalmol"},{"key":"bib28","doi-asserted-by":"publisher","first-page":"917","DOI":"10.1016\/j.ajo.2011.05.019","article-title":"Clinical outcomes of penetrating keratoplasty after autologous cultivated limbal epithelial transplantation for ocular surface burns","volume":"152","author":"Basu","year":"2011","journal-title":"Am J Ophthalmol"},{"key":"bib29","doi-asserted-by":"crossref","first-page":"202","DOI":"10.4103\/0301-4738.99840","article-title":"Coculture of autologous limbal and conjunctival epithelial cells to treat severe ocular surface disorders: long-term survival analysis","volume":"61","author":"Subramaniam","year":"2013","journal-title":"Indian J Ophthalmol"},{"key":"bib30","doi-asserted-by":"crossref","first-page":"644","DOI":"10.4103\/0301-4738.109517","article-title":"Oral epithelial cells transplanted on to corneal surface tend to adapt to the ocular phenotype","volume":"62","author":"Gaddipati","year":"2014","journal-title":"Indian J Ophthalmol"},{"key":"bib31","doi-asserted-by":"publisher","first-page":"1640","DOI":"10.1136\/bjophthalmol-2017-311506","article-title":"Simple limbal epithelial transplantation (SLET) in failed cultivated limbal epithelial transplantation (CLET) for unilateral chronic ocular burns","volume":"102","author":"Basu","year":"2018","journal-title":"Br J Ophthalmol"},{"key":"bib32","doi-asserted-by":"publisher","first-page":"1000","DOI":"10.1016\/j.ophtha.2015.12.042","article-title":"Simple limbal epithelial transplantation: long-term clinical outcomes in 125 cases of unilateral chronic ocular surface burns","volume":"123","author":"Basu","year":"2016","journal-title":"Ophthalmology"},{"key":"bib33","doi-asserted-by":"publisher","first-page":"757","DOI":"10.1016\/j.ajo.2006.06.004","article-title":"Ocular surface reconstruction with combination of cultivated autologous oral mucosal epithelial transplantation and penetrating keratoplasty","volume":"142","author":"Inatomi","year":"2006","journal-title":"Am J Ophthalmol"},{"key":"bib34","doi-asserted-by":"publisher","first-page":"11522","DOI":"10.3390\/ijms241411522","article-title":"Comparison between cultivated oral mucosa and ocular surface epithelia for COMET patients follow-up","volume":"24","author":"Attico","year":"2023","journal-title":"Int J Mol Sci"},{"key":"bib35","first-page":"281","article-title":"Amniotic membrane transplantation for reconstruction of corneal epithelial surface in cases of partial limbal stem cell deficiency","volume":"52","author":"Sangwan","year":"2004","journal-title":"Indian J Ophthalmol"},{"key":"bib36","doi-asserted-by":"crossref","first-page":"201","DOI":"10.4103\/0028-3886.63789","article-title":"Isolation, characterization and differentiation potential of rat bone marrow stromal cells","volume":"58","author":"Polisetti","year":"2010","journal-title":"Neurol India"},{"issue":"5","key":"bib38","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1016\/j.jcyt.2025.01.014","article-title":"Advancing mesenchymal stromal cell therapy: The crucial role of updated guidelines","volume":"27","author":"Wiest","year":"2025","journal-title":"Cytotherapy"},{"key":"bib39","first-page":"1227","article-title":"Gene expression profile of epithelial cells and mesenchymal cells derived from limbal explant culture","volume":"16","author":"Polisetti","year":"2010","journal-title":"Mol Vis"},{"key":"bib40","first-page":"431","article-title":"Mesenchymal cells from limbal stroma of human eye","volume":"14","author":"Polisetty","year":"2008","journal-title":"Mol Vis"},{"key":"bib41","doi-asserted-by":"publisher","first-page":"266ra172","DOI":"10.1126\/scitranslmed.3009644","article-title":"Human limbal biopsy-derived stromal stem cells prevent corneal scarring","volume":"6","author":"Basu","year":"2014","journal-title":"Sci Transl Med"},{"key":"bib42","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1007\/s00417-018-3926-8","article-title":"Establishing and characterizing lacrispheres from human lacrimal gland for potential clinical application","volume":"256","author":"Tiwari","year":"2018","journal-title":"Graefes Arch Clin Exp Ophthalmol"},{"key":"bib43","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1167\/iovs.64.10.12","article-title":"Human lacrimal gland derived mesenchymal stem cells - isolation, propagation, and characterization","volume":"64","author":"Jaffet","year":"2023","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"bib44","doi-asserted-by":"publisher","first-page":"4003","DOI":"10.1158\/0008-5472.CAN-15-0798","article-title":"Cell-of-origin of cancer versus cancer stem cells: assays and interpretations","volume":"75","author":"Rycaj","year":"2015","journal-title":"Cancer Res"},{"key":"bib45","doi-asserted-by":"publisher","first-page":"10697","DOI":"10.18632\/oncotarget.4037","article-title":"EMT, CTCs and CSCs in tumor relapse and drug-resistance","volume":"6","author":"Mitra","year":"2015","journal-title":"Oncotarget"},{"key":"bib46","doi-asserted-by":"publisher","first-page":"440","DOI":"10.1016\/j.ccell.2016.03.009","article-title":"Intratumoral heterogeneity of the epigenome","volume":"29","author":"Mazor","year":"2016","journal-title":"Cancer Cell"},{"key":"bib47","first-page":"105","article-title":"Tumor heterogeneity: causes and consequences","volume":"1805","author":"Marusyk","year":"2010","journal-title":"Biochim Biophys Acta"},{"key":"bib48","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1016\/j.stem.2014.02.006","article-title":"Evolution of the cancer stem cell model","volume":"14","author":"Kreso","year":"2014","journal-title":"Cell Stem Cell"},{"key":"bib49","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1007\/s10555-022-10073-z","article-title":"Dormant cancer cells: programmed quiescence, senescence, or both?","volume":"42","author":"Truskowski","year":"2023","journal-title":"Cancer Metastasis Rev"},{"key":"bib50","doi-asserted-by":"publisher","first-page":"3822","DOI":"10.3390\/cancers16223822","article-title":"Tracing quiescent cancer cells in vivo","volume":"16","author":"Kim","year":"2024","journal-title":"Cancers"},{"key":"bib51","doi-asserted-by":"publisher","first-page":"1506","DOI":"10.1167\/iovs.08-2356","article-title":"Phenotypic characterization of retinoblastoma for the presence of putative cancer stem-like cell markers by flow cytometry","volume":"50","author":"Balla","year":"2009","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"bib52","doi-asserted-by":"publisher","first-page":"779","DOI":"10.1186\/s12885-017-3750-2","article-title":"In vitro characterization of CD133(lo) cancer stem cells in Retinoblastoma Y79 cell line","volume":"17","author":"Nair","year":"2017","journal-title":"BMC Cancer"},{"key":"bib53","doi-asserted-by":"publisher","first-page":"1703","DOI":"10.4103\/ijo.IJO_2348_21","article-title":"A short-term chick embryo in vivo xenograft model to study retinoblastoma cancer stem cells","volume":"70","author":"Nair","year":"2022","journal-title":"Indian J Ophthalmol"},{"key":"bib54","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1167\/iovs.62.14.13","article-title":"Carboplatin- and etoposide-loaded lactoferrin protein nanoparticles for targeting cancer stem cells in retinoblastoma in vitro","volume":"62","author":"Narayana","year":"2021","journal-title":"Invest Ophthalmol Vis Science"},{"key":"bib55","doi-asserted-by":"publisher","first-page":"116403","DOI":"10.1016\/j.biopha.2024.116403","article-title":"Small extracellular vesicles loaded with carboplatin effectively enhance the cytotoxicity of drug-resistant cells from Y79 cells-in vitro","volume":"173","author":"Attem","year":"2024","journal-title":"Biomed Pharmacother"},{"key":"bib56","doi-asserted-by":"publisher","first-page":"1264","DOI":"10.1080\/02713683.2025.2457102","article-title":"The role of small extracellular vesicles in retinoblastoma development and progression","volume":"50","author":"Attem","year":"2025","journal-title":"Curr Eye Res"},{"key":"bib57","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1167\/iovs.64.11.26","article-title":"Identifying treatment resistance related pathways by analyzing serum extracellular vesicles of patients with resistant versus regressed retinoblastoma","volume":"64","author":"Manukonda","year":"2023","journal-title":"Invest Ophthalmol Vis Science"},{"key":"bib58","doi-asserted-by":"publisher","first-page":"4179","DOI":"10.3390\/cancers14174179","article-title":"Comprehensive analysis of serum small extracellular vesicles-derived coding and non-coding RNAs from retinoblastoma patients for identifying regulatory interactions","volume":"14","author":"Manukonda","year":"2022","journal-title":"Cancers (Basel)"},{"key":"bib59","first-page":"36","article-title":"The effect of hBM-MSC-derived small extracellular vesicles (sEVs) on RbY79 cells","volume":"66","author":"Vemuganti","year":"2025","journal-title":"Invest Ophthalmol Vis Science."},{"key":"bib1","first-page":"79","article-title":"Tissue renewal, regeneration and repair","volume-title":"Pathologic Basis of Disease","author":"Kumar","year":"2010","edition":"Eighth ed."},{"key":"bib37","first-page":"275","article-title":"Unstimulated diagnostic marrow tap \u2013 a minimally invasive and reliable source for mesenchymal stem cells","volume-title":"Cell Biol Int","author":"Koppula","year":"2010"}],"container-title":["Investigative Ophthalmology &amp; Visual Science"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/iovs.arvojournals.org\/article.aspx?doi=10.1167\/iovs.67.12.11","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T21:32:13Z","timestamp":1791927133000},"score":1,"resource":{"primary":{"URL":"http:\/\/iovs.arvojournals.org\/article.aspx?doi=10.1167\/iovs.67.12.11"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,6]]},"references-count":59,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2026,10,1]]}},"URL":"https:\/\/doi.org\/10.1167\/iovs.67.12.11","relation":{},"ISSN":["1552-5783"],"issn-type":[{"value":"1552-5783","type":"print"}],"subject":[],"published":{"date-parts":[[2026,10,6]]}}}