{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T22:02:34Z","timestamp":1791928954593,"version":"4.3.4"},"reference-count":54,"publisher":"Association for Research in Vision and Ophthalmology (ARVO)","issue":"2","license":[{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Invest. Ophthalmol. Vis. Sci."],"DOI":"10.1167\/iovs.67.2.13","type":"journal-article","created":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T13:30:56Z","timestamp":1770211856000},"page":"13","source":"Crossref","is-referenced-by-count":1,"title":["Time-Dependent Glucocorticoid-Induced Transcriptomic Changes in Human Trabecular Meshwork and Schlemm's Canal Cells"],"prefix":"10.1167","volume":"67","author":[{"given":"Sudeep","family":"Mehrotra","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts, United States"},{"name":"Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haven","family":"Jeanneret","sequence":"additional","affiliation":[{"name":"Department of Molecular Biomedical Sciences, North Carolina State University, Raleigh, North Carolina, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristin","family":"Perkumas","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Duke University, Durham, North Carolina, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renee","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jyoti","family":"Lama","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts, United States"},{"name":"Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katie","family":"Huynh","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ananya","family":"Mukundan","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hilary","family":"Scott","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atitaya","family":"Apivatthakakul","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janey L.","family":"Wiggs","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts, United States"},{"name":"Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Sobrin","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts, United States"},{"name":"Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. Daniel","family":"Stamer","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Duke University, Durham, North Carolina, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayellet V.","family":"Segr\u00e8","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts, United States"},{"name":"Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"27","published-online":{"date-parts":[[2026,2,4]]},"reference":[{"issue":"2","key":"bib1","first-page":"43","article-title":"Comprehension of top 200 prescribed drugs in the US as a resource for pharmacy teaching, training and practice","volume":"6","author":"Fuentes","year":"2018","journal-title":"Pharmacy (Basel)"},{"issue":"3","key":"bib2","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1001\/archophthalmol.2008.610","article-title":"Three-year follow-up of a randomized trial comparing focal\/grid photocoagulation and intravitreal triamcinolone for diabetic macular edema","volume":"127","author":"Diabetic Retinopathy Clinical Research Network","year":"2009","journal-title":"Arch Ophthalmol"},{"issue":"3","key":"bib3","doi-asserted-by":"publisher","first-page":"262","DOI":"10.1136\/bjo.2005.081224","article-title":"The number of people with glaucoma worldwide in 2010 and 2020","volume":"90","author":"Quigley","year":"2006","journal-title":"Br J Ophthalmol"},{"issue":"11","key":"bib4","doi-asserted-by":"publisher","first-page":"2081","DOI":"10.1016\/j.ophtha.2014.05.013","article-title":"Global prevalence of glaucoma and projections of glaucoma burden through 2040: a systematic review and meta-analysis","volume":"121","author":"Tham","year":"2014","journal-title":"Ophthalmology"},{"key":"bib5","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.ajo.2019.02.001","article-title":"The genetic influence on corticosteroid-induced ocular hypertension: a field positioned for discovery","volume":"202","author":"Chan","year":"2019","journal-title":"Am J Ophthalmol"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1016\/j.preteyeres.2016.09.003","article-title":"Steroid-induced ocular hypertension\/glaucoma: focus on pharmacogenomics and implications for precision medicine","volume":"56","author":"Fini","year":"2017","journal-title":"Prog Retin Eye Res"},{"issue":"1","key":"bib7","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1001\/archopht.1966.00970050034007","article-title":"The heritable nature of dexamethasone-induced ocular hypertension","volume":"75","author":"Armaly","year":"1966","journal-title":"Arch Ophthalmol"},{"issue":"4","key":"bib8","doi-asserted-by":"publisher","first-page":"484","DOI":"10.1001\/archopht.1966.03850010486004","article-title":"Topical corticosteroid testing in glaucoma siblings","volume":"76","author":"Becker","year":"1966","journal-title":"Arch Ophthalmol"},{"key":"bib9","doi-asserted-by":"publisher","first-page":"543","DOI":"10.1016\/0002-9394(64)92500-0","article-title":"Topical corticosteroids and heredity in primary open-angle glaucoma","volume":"57","author":"Becker","year":"1964","journal-title":"Am J Ophthalmol"},{"issue":"4","key":"bib10","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1001\/archopht.1973.01000050283006","article-title":"Twin study on ocular pressure following topically applied dexamethasone. II. Inheritance of variation in pressure response","volume":"90","author":"Schwartz","year":"1973","journal-title":"Arch Ophthalmol"},{"issue":"3","key":"bib11","doi-asserted-by":"publisher","first-page":"e1002637","DOI":"10.1371\/journal.pgen.1002637","article-title":"Heritability and genetic correlations explained by common SNPs for metabolic syndrome traits","volume":"8","author":"Vattikuti","year":"2012","journal-title":"PLoS Genet"},{"issue":"11","key":"bib12","doi-asserted-by":"publisher","first-page":"1113","DOI":"10.1056\/NEJMra0804630","article-title":"Primary open-angle glaucoma","volume":"360","author":"Kwon","year":"2009","journal-title":"N Engl J Med"},{"issue":"10","key":"bib13","doi-asserted-by":"publisher","first-page":"3877","DOI":"10.1172\/JCI94668","article-title":"Impaired angiopoietin\/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma","volume":"127","author":"Kim","year":"2017","journal-title":"J Clin Invest"},{"key":"bib14","first-page":"2001","article-title":"Proteomic similarities in steroid responsiveness in normal and glaucomatous trabecular meshwork cells","volume":"18","author":"Bollinger","year":"2012","journal-title":"Mol Vis"},{"issue":"2","key":"bib15","doi-asserted-by":"publisher","first-page":"857","DOI":"10.1167\/iovs.11-8497","article-title":"Spliceosome protein (SRp) regulation of glucocorticoid receptor isoforms and glucocorticoid response in human trabecular meshwork cells","volume":"53","author":"Jain","year":"2012","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"2","key":"bib16","doi-asserted-by":"publisher","first-page":"C330","DOI":"10.1152\/ajpcell.1999.277.2.C330","article-title":"Glucocorticoids regulate transendothelial fluid flow resistance and formation of intercellular junctions","volume":"277","author":"Underwood","year":"1999","journal-title":"Am J Physiol"},{"issue":"5","key":"bib17","doi-asserted-by":"publisher","first-page":"882","DOI":"10.3390\/genes13050882","article-title":"A comparative genome-wide transcriptome analysis of glucocorticoid responder and non-responder primary human trabecular meshwork cells","volume":"13","author":"Kathirvel","year":"2022","journal-title":"Genes (Basel)"},{"issue":"8","key":"bib18","doi-asserted-by":"publisher","first-page":"1240","DOI":"10.18240\/ijo.2022.08.03","article-title":"Expression profile analysis to identify potential gene changes induced by dexamethasone in the trabecular meshwork","volume":"15","author":"Wei","year":"2022","journal-title":"Int J Ophthalmol"},{"key":"bib19","doi-asserted-by":"publisher","first-page":"9636897231177356","DOI":"10.1177\/09636897231177356","article-title":"Dexamethasone induces senescence-associated changes in trabecular meshwork cells by increasing ROS levels via the TGF\u00df\/Smad3-NOX4 axis","volume":"32","author":"Li","year":"2023","journal-title":"Cell Transplant"},{"key":"bib20","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1016\/j.exer.2018.03.001","article-title":"Consensus recommendations for trabecular meshwork cell isolation, characterization and culture","volume":"171","author":"Keller","year":"2018","journal-title":"Exp Eye Res"},{"issue":"1","key":"bib21","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1016\/j.exer.2011.12.017","article-title":"Protein markers and differentiation in culture for Schlemm's canal endothelial cells","volume":"96","author":"Perkumas","year":"2012","journal-title":"Exp Eye Res"},{"issue":"10","key":"bib22","first-page":"1804","article-title":"Isolation, culture, and characterization of endothelial cells from Schlemm's canal","volume":"39","author":"Stamer","year":"1998","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"19","key":"bib23","doi-asserted-by":"publisher","first-page":"10339","DOI":"10.1073\/pnas.2001250117","article-title":"Cell atlas of aqueous humor outflow pathways in eyes of humans and four model species provides insight into glaucoma pathogenesis","volume":"117","author":"van Zyl","year":"2020","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"1","key":"bib24","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: ultrafast universal RNA-seq aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics"},{"issue":"10","key":"bib25","doi-asserted-by":"publisher","first-page":"e108","DOI":"10.1093\/nar\/gkt214","article-title":"The Subread aligner: fast, accurate and scalable read mapping by seed-and-vote","volume":"41","author":"Liao","year":"2013","journal-title":"Nucleic Acids Res"},{"issue":"12","key":"bib26","doi-asserted-by":"publisher","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol"},{"issue":"1","key":"bib27","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1186\/s12864-017-3808-1","article-title":"Fast and robust adjustment of cell mixtures in epigenome-wide association studies with SmartSVA","volume":"18","author":"Chen","year":"2017","journal-title":"BMC Genomics"},{"issue":"W1","key":"bib28","doi-asserted-by":"publisher","first-page":"W191","DOI":"10.1093\/nar\/gkz369","article-title":"g:Profiler: a web server for functional enrichment analysis and conversions of gene lists (2019 update)","volume":"47","author":"Raudvere","year":"2019","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"bib29","doi-asserted-by":"publisher","first-page":"1523","DOI":"10.1038\/s41467-019-09234-6","article-title":"Metascape provides a biologist-oriented resource for the analysis of systems-level datasets","volume":"10","author":"Zhou","year":"2019","journal-title":"Nat Commun"},{"issue":"12","key":"bib30","doi-asserted-by":"publisher","first-page":"1739","DOI":"10.1093\/bioinformatics\/btr260","article-title":"Molecular signatures database (MSigDB) 3.0","volume":"27","author":"Liberzon","year":"2011","journal-title":"Bioinformatics"},{"key":"bib31","doi-asserted-by":"publisher","first-page":"638255","DOI":"10.3389\/fbinf.2021.638255","article-title":"Summary visualizations of Gene Ontology terms with GO-Figure!","volume":"1","author":"Reijnders","year":"2021","journal-title":"Front Bioinform"},{"key":"bib32","doi-asserted-by":"publisher","first-page":"bar030","DOI":"10.1093\/database\/bar030","article-title":"Ensembl BioMarts: a hub for data retrieval across taxonomic space","volume":"2011","author":"Kinsella","year":"2011","journal-title":"Database (Oxford)"},{"key":"bib33","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1080\/10618600.1996.10474713","article-title":"R: A language for data analysis and graphics","volume":"5","author":"Ihaka","year":"1996","journal-title":"J Comput Graph Stat"},{"issue":"1","key":"bib35","doi-asserted-by":"publisher","first-page":"396","DOI":"10.1038\/s41467-023-44380-y","article-title":"Integrating genetic regulation and single-cell expression with GWAS prioritizes causal genes and cell types for glaucoma","volume":"15","author":"Hamel","year":"2024","journal-title":"Nat Commun"},{"issue":"1","key":"bib36","doi-asserted-by":"publisher","first-page":"1258","DOI":"10.1038\/s41467-020-20851-4","article-title":"Genome-wide meta-analysis identifies 127 open-angle glaucoma loci with consistent effect across ancestries","volume":"12","author":"Gharahkhani","year":"2021","journal-title":"Nat Commun"},{"issue":"7","key":"bib37","doi-asserted-by":"publisher","first-page":"1116","DOI":"10.1038\/s41588-023-01428-5","article-title":"Large-scale multitrait genome-wide association analyses identify hundreds of glaucoma risk loci","volume":"55","author":"Han","year":"2023","journal-title":"Nat Genet"},{"issue":"6","key":"bib38","doi-asserted-by":"publisher","first-page":"778","DOI":"10.1038\/s41588-018-0126-8","article-title":"Genome-wide analyses identify 68 new loci associated with intraocular pressure and improve risk prediction for primary open-angle glaucoma","volume":"50","author":"Khawaja","year":"2018","journal-title":"Nat Genet"},{"issue":"12","key":"bib39","doi-asserted-by":"publisher","first-page":"2205","DOI":"10.1093\/hmg\/ddy111","article-title":"Genome-wide association analyses identify new loci influencing intraocular pressure","volume":"27","author":"Gao","year":"2018","journal-title":"Hum Mol Genet"},{"issue":"2","key":"bib40","doi-asserted-by":"publisher","first-page":"114220","DOI":"10.1016\/j.yexcr.2024.114220","article-title":"RNA-seq transcriptomic profiling of TGF-\u00df2-exposed human trabecular meshwork explants: Advancing insights beyond conventional cell culture models","volume":"442","author":"Buffault","year":"2024","journal-title":"Exp Cell Res"},{"issue":"8","key":"bib41","doi-asserted-by":"publisher","first-page":"4395","DOI":"10.1128\/MCB.15.8.4395","article-title":"FKBP51, a novel T-cell-specific immunophilin capable of calcineurin inhibition","volume":"15","author":"Baughman","year":"1995","journal-title":"Mol Cell Biol"},{"issue":"3","key":"bib42","doi-asserted-by":"publisher","first-page":"1037","DOI":"10.1167\/iovs.07-1279","article-title":"FK506-binding protein 51 regulates nuclear transport of the glucocorticoid receptor \u00df and glucocorticoid responsiveness","volume":"49","author":"Zhang","year":"2008","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"bib43","first-page":"596","article-title":"Assessment of SNPs associated with the human glucocorticoid receptor in primary open-angle glaucoma and steroid responders","volume":"16","author":"Fingert","year":"2010","journal-title":"Mol Vis"},{"issue":"3","key":"bib44","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1167\/iovs.61.3.26","article-title":"Circadian regulation of IOP rhythm by dual pathways of glucocorticoids and the sympathetic nervous system","volume":"61","author":"Ikegami","year":"2020","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"4","key":"bib45","doi-asserted-by":"publisher","first-page":"2657","DOI":"10.1167\/iovs.15-16449","article-title":"Analysis of circadian rhythm gene expression with reference to diurnal pattern of intraocular pressure in mice","volume":"56","author":"Dalvin","year":"2015","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"3","key":"bib46","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1016\/S0014-5793(98)00791-1","article-title":"Identification and functional characterization of DR6, a novel death domain-containing TNF receptor","volume":"431","author":"Pan","year":"1998","journal-title":"FEBS Lett"},{"issue":"4","key":"bib47","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1167\/iovs.61.4.24","article-title":"The molecular processes in the trabecular meshwork after exposure to corticosteroids and in corticosteroid-induced ocular hypertension","volume":"61","author":"Liesenborghs","year":"2020","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"10","key":"bib48","doi-asserted-by":"publisher","first-page":"7862","DOI":"10.1007\/s12035-024-04036-4","article-title":"SOX9 promotes collagen VI secretion by upregulating PCOLCE in neurofibroma","volume":"61","author":"Yao","year":"2024","journal-title":"Mol Neurobiol"},{"issue":"10","key":"bib49","doi-asserted-by":"publisher","first-page":"1120","DOI":"10.1038\/ng.3079","article-title":"Common variants near ABCA1, AFAP1 and GMDS confer risk of primary open-angle glaucoma","volume":"46","author":"Gharahkhani","year":"2014","journal-title":"Nat Genet"},{"issue":"7","key":"bib50","doi-asserted-by":"publisher","first-page":"1021","DOI":"10.1007\/s00417-008-0800-0","article-title":"Immunohistochemical evaluation of the extracellular matrix in trabecular meshwork in steroid-induced glaucoma","volume":"246","author":"Tawara","year":"2008","journal-title":"Graefes Arch Clin Exp Ophthalmol"},{"issue":"8","key":"bib51","doi-asserted-by":"publisher","first-page":"4447","DOI":"10.1167\/iovs.15-16739","article-title":"Dexamethasone stiffens trabecular meshwork, trabecular meshwork cells, and matrix","volume":"56","author":"Raghunathan","year":"2015","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"12","key":"bib52","first-page":"3544","article-title":"Bidrectional interactions between trabecular meshwork cells and their extracellular matrix in the presence of dexamethasone","volume":"57","author":"Raghunathan","year":"2016","journal-title":"Invest Ophthalmol"},{"issue":"1","key":"bib53","doi-asserted-by":"publisher","first-page":"3124","DOI":"10.1038\/s41598-018-20435-9","article-title":"Analysis combining correlated glaucoma traits identifies five new risk loci for open-angle glaucoma","volume":"8","author":"Gharahkhani","year":"2018","journal-title":"Sci Rep"},{"issue":"9","key":"bib54","doi-asserted-by":"publisher","first-page":"3701","DOI":"10.1172\/JCI77507","article-title":"Schlemm's canal: more than meets the eye, lymphatics in disguise","volume":"124","author":"Karpinich","year":"2014","journal-title":"J Clin Invest"},{"key":"bib34","article-title":"gglpot2: create elegant data visualisations using the grammar of graphics","author":"Wickham"}],"container-title":["Investigative Ophthalmology &amp; Visual Science"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/iovs.arvojournals.org\/article.aspx?doi=10.1167\/iovs.67.2.13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T21:32:14Z","timestamp":1791927134000},"score":1,"resource":{"primary":{"URL":"http:\/\/iovs.arvojournals.org\/article.aspx?doi=10.1167\/iovs.67.2.13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,4]]},"references-count":54,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2,2]]}},"URL":"https:\/\/doi.org\/10.1167\/iovs.67.2.13","relation":{},"ISSN":["1552-5783"],"issn-type":[{"value":"1552-5783","type":"print"}],"subject":[],"published":{"date-parts":[[2026,2,4]]}}}