{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T01:58:33Z","timestamp":1769911113572,"version":"3.49.0"},"reference-count":65,"publisher":"Association for Research in Vision and Ophthalmology (ARVO)","issue":"8","license":[{"start":{"date-parts":[[2021,7,13]],"date-time":"2021-07-13T00:00:00Z","timestamp":1626134400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Trans. Vis. Sci. Tech."],"published-print":{"date-parts":[[2021,7,13]]},"DOI":"10.1167\/tvst.10.8.14","type":"journal-article","created":{"date-parts":[[2021,7,13]],"date-time":"2021-07-13T14:31:19Z","timestamp":1626186679000},"page":"14","source":"Crossref","is-referenced-by-count":16,"title":["Long-Term Decrease of Intraocular Pressure in Rats by Viral Delivery of miR-146a"],"prefix":"10.1167","volume":"10","author":[{"given":"Coralia","family":"Luna","sequence":"first","affiliation":[{"name":"Duke University Eye Center, Durham, NC, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Megan","family":"Parker","sequence":"additional","affiliation":[{"name":"Duke University Eye Center, Durham, NC, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pratap","family":"Challa","sequence":"additional","affiliation":[{"name":"Duke University Eye Center, Durham, NC, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Duke University Eye Center, Durham, NC, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"27","reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"724-730","DOI":"10.1016\/j.ajo.2013.05.027","article-title":"Lifetime risk of blindness in open-angle glaucoma","volume":"156","author":"Peters","year":"2013","journal-title":"Am J Ophthalmol"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"31-53","DOI":"10.1016\/j.preteyeres.2018.08.003","article-title":"Gene therapy for visual loss: Opportunities and concerns","volume":"68","author":"Lee","year":"2019","journal-title":"Prog Retin Eye Res"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"226-232","DOI":"10.1038\/mt.2013.217","article-title":"Phase I clinical trial of SYL040012, a small interfering RNA targeting \u00df-adrenergic receptor 2, for lowering intraocular pressure","volume":"22","author":"Moreno-Monta\u00f1\u00e9s","year":"2014","journal-title":"Mol Ther"},{"key":"bib4","doi-asserted-by":"publisher","first-page":"191-200","DOI":"10.1089\/hum.2008.086","article-title":"Prolonged transgene expression with lentiviral vectors in the aqueous humor outflow pathway of nonhuman primates","volume":"20","author":"Barraza","year":"2009","journal-title":"Hum Gene Ther"},{"key":"bib5","doi-asserted-by":"publisher","first-page":"3029-3041","DOI":"10.1167\/iovs.09-4918","article-title":"Development of a gene therapy virus with a glucocorticoid-inducible MMP1 for the treatment of steroid glaucoma","volume":"51","author":"Spiga","year":"2010","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"491-501","DOI":"10.1038\/mt.2009.278","article-title":"Prostaglandin pathway gene therapy for sustained reduction of intraocular pressure","volume":"18","author":"Barraza","year":"2010","journal-title":"Mol Ther"},{"key":"bib7","doi-asserted-by":"crossref","first-page":"3042-3048","DOI":"10.1167\/iovs.09-4920","article-title":"Treatment of sheep steroid-induced ocular hypertension with a glucocorticoid-inducible MMP1 gene therapy virus","volume":"51","author":"Gerometta","year":"2010","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"bib8","doi-asserted-by":"publisher","first-page":"353-362","DOI":"10.1016\/j.sjopt.2011.07.005","article-title":"Gene therapy strategies in glaucoma and application for steroid-induced hypertension","volume":"25","author":"Borr\u00e1s","year":"2011","journal-title":"Saudi J Ophthalmol"},{"key":"bib9","doi-asserted-by":"publisher","first-page":"e72447","DOI":"10.1371\/journal.pone.0072447","article-title":"Tissue plasminogen activator in trabecular meshwork attenuates steroid induced outflow resistance in mice","volume":"8","author":"Kumar","year":"2013","journal-title":"PLoS One"},{"key":"bib10","doi-asserted-by":"publisher","first-page":"438-449","DOI":"10.1038\/gt.2016.14","article-title":"Inducible scAAV2.GRE.MMP1 lowers IOP long-term in a large animal model for steroid-induced glaucoma gene therapy","volume":"23","author":"Borr\u00e1s","year":"2016","journal-title":"Gene Ther"},{"key":"bib11","first-page":"80-93","article-title":"The pathway from genes to gene therapy in glaucoma: a review of possibilities for using genes as glaucoma drugs","volume":"6","author":"Borr\u00e1s","year":"2017","journal-title":"Asia Pac J Ophthalmol (Phila)"},{"key":"bib12","doi-asserted-by":"publisher","first-page":"e1803239","DOI":"10.1002\/smll.v14.50","article-title":"Intracameral delivery of layer-by-layer coated siRNA nanoparticles for glaucoma therapy","volume":"14","author":"Dillinger","year":"2018","journal-title":"Small"},{"key":"bib13","first-page":"1037-1050","article-title":"Open-angle glaucoma: therapeutically targeting the extracellular matrix of the conventional outflow pathway","volume":"21","author":"O'Callaghan","year":"2017","journal-title":"Expert Opin Ther Targets"},{"key":"bib14","first-page":"1230-1246","article-title":"Therapeutic potential of AAV-mediated MMP-3 secretion from corneal endothelium in treating glaucoma","volume":"26","author":"O'Callaghan","year":"2017","journal-title":"Hum Mol Genet"},{"key":"bib15","doi-asserted-by":"publisher","first-page":"1327-1338","DOI":"10.1016\/j.ymthe.2019.04.021","article-title":"Lentiviral vector-mediated expression of exoenzyme C3 transferase lowers intraocular pressure in monkeys","volume":"27","author":"Tan","year":"2019","journal-title":"Mol Ther"},{"key":"bib16","doi-asserted-by":"crossref","first-page":"4937-4944","DOI":"10.1167\/iovs.18-24978","article-title":"Effects of lentivirus-mediated C3 expression on trabecular meshwork cells and intraocular pressure","volume":"59","author":"Tan","year":"2018","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"bib17","doi-asserted-by":"crossref","first-page":"859-870","DOI":"10.3109\/02713683.2014.884593","article-title":"Prospects for lentiviral vector mediated prostaglandin F synthase gene delivery in monkey eyes in vivo","volume":"39","author":"Lee","year":"2014","journal-title":"Curr Eye Res"},{"key":"bib18","doi-asserted-by":"publisher","first-page":"2431-2438","DOI":"10.1167\/iovs.14-15909","article-title":"Viral vector effects on exoenzyme C3 transferase-mediated actin disruption and on outflow facility","volume":"56","author":"Slauson","year":"2015","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"bib19","first-page":"924-929","article-title":"Gene transfer to human trabecular meshwork cells in vitro and ex vivo using HIV-based lentivirus","volume":"7","author":"Xiang","year":"2014","journal-title":"Int J Ophthalmol"},{"issue":"3","key":"bib20","doi-asserted-by":"publisher","first-page":"820-829","DOI":"10.1016\/j.ymthe.2019.12.012","article-title":"Gene therapy for glaucoma by ciliary body aquaporin 1 disruption using CRISPR-Cas9","volume":"28","author":"Wu","year":"2020","journal-title":"Mol Ther"},{"issue":"3","key":"bib21","doi-asserted-by":"publisher","first-page":"706-708","DOI":"10.1016\/j.ymthe.2020.02.011","article-title":"CRISPR-Cas9 Disruption of aquaporin 1: an alternative to glaucoma eye drop therapy?","volume":"28","author":"Kom\u00e1romy","year":"2020","journal-title":"Mol Ther"},{"key":"bib22","doi-asserted-by":"publisher","first-page":"313-324","DOI":"10.1016\/j.cjca.2017.01.001","article-title":"MicroRNAs in the pathobiology and therapy of atherosclerosis","volume":"33","author":"Laffont","year":"2017","journal-title":"Can J Cardiol"},{"key":"bib23","doi-asserted-by":"publisher","first-page":"128-141","DOI":"10.1016\/j.addr.2014.05.009","article-title":"In vivo delivery of miRNAs for cancer therapy: challenges and strategies","volume":"81","author":"Chen","year":"2015","journal-title":"Adv Drug Deliv Rev"},{"key":"bib24","doi-asserted-by":"publisher","first-page":"2277-2288","DOI":"10.1681\/ASN.2015010111","article-title":"Anti-inflammatory role of microRNA-146a in the pathogenesis of diabetic nephropathy","volume":"27","author":"Bhatt","year":"2016","journal-title":"J Am Soc Nephrol"},{"key":"bib25","doi-asserted-by":"publisher","first-page":"4944-4951","DOI":"10.1167\/iovs.13-13631","article-title":"MicroRNA-146 inhibits thrombin-induced NF-?B activation and subsequent inflammatory responses in human retinal endothelial cells","volume":"55","author":"Cowan","year":"2014","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"bib26","doi-asserted-by":"publisher","first-page":"e0173918","DOI":"10.1371\/journal.pone.0173918","article-title":"miR-146a regulates glucose induced upregulation of inflammatory cytokines extracellular matrix proteins in the retina and kidney in diabetes","volume":"12","author":"Chen","year":"2017","journal-title":"PLoS One"},{"key":"bib27","doi-asserted-by":"publisher","first-page":"70-76","DOI":"10.1016\/j.yjmcc.2017.03.002","article-title":"miR-146a mediates inflammatory changes and fibrosis in the heart in diabetes","volume":"105","author":"Feng","year":"2017","journal-title":"J Mol Cell Cardiol"},{"key":"bib28","doi-asserted-by":"publisher","first-page":"12626","DOI":"10.1038\/s41598-019-49090-4","article-title":"miR-146a deficiency accelerates hepatic inflammation without influencing diet-induced obesity in mice","volume":"9","author":"Javidan","year":"2019","journal-title":"Sci Rep"},{"key":"bib29","doi-asserted-by":"crossref","first-page":"2976-2985","DOI":"10.1167\/iovs.09-4874","article-title":"Modulation of inflammatory markers by miR-146a during replicative senescence in trabecular meshwork cells","volume":"51","author":"Li","year":"2010","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"bib30","doi-asserted-by":"publisher","first-page":"1407-1414","DOI":"10.1002\/jcp.22476","article-title":"MicroRNA-24 regulates the processing of latent TGF\u00df1 during cyclic mechanical stress in human trabecular meshwork cells through direct targeting of FURIN","volume":"226","author":"Luna","year":"2011","journal-title":"J Cell Physiol"},{"key":"bib31","doi-asserted-by":"publisher","first-page":"239-245","DOI":"10.1161\/01.RES.72.2.239","article-title":"Mechanical stress mechanisms and the cell. An endothelial paradigm","volume":"72","author":"Davies","year":"1993","journal-title":"Circ Res"},{"key":"bib32","doi-asserted-by":"publisher","first-page":"1-16","DOI":"10.1007\/s10237-005-0012-z","article-title":"An introductory review of cell mechanobiology","volume":"5","author":"Wang","year":"2006","journal-title":"Biomech Model Mechanobiol"},{"key":"bib33","first-page":"378-385","article-title":"Stress response of the trabecular meshwork","volume":"7","author":"Liton","year":"2008","journal-title":"J Glaucoma"},{"key":"bib34","doi-asserted-by":"publisher","first-page":"2857-2868","DOI":"10.1167\/iovs.05-0075","article-title":"Changes in gene expression by trabecular meshwork cells in response to mechanical stretching","volume":"46","author":"Vittal","year":"2005","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"bib35","first-page":"352-361","article-title":"Genes upregulated in the human trabecular meshwork in response to elevated intraocular pressure","volume":"41","author":"Gonzalez","year":"2000","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"bib36","first-page":"1361-1371","article-title":"Mechanical stretch alters the actin cytoskeletal network and signal transduction in human trabecular meshwork cells","volume":"39","author":"Tumminia","year":"1998","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"bib37","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1002\/jcp.v201:1","article-title":"Genes expressed in the human trabecular meshwork during pressure- induced homeostatic response","volume":"201","author":"Vittitow","year":"2004","journal-title":"J Cell Physiol"},{"key":"bib38","doi-asserted-by":"publisher","first-page":"164-173","DOI":"10.1016\/j.exer.2018.03.001","article-title":"Consensus recommendations for trabecular meshwork cell isolation, characterization and culture","volume":"171","author":"Keller","year":"2018","journal-title":"Exp Eye Res"},{"key":"bib39","doi-asserted-by":"publisher","first-page":"e115","DOI":"10.1093\/nar\/gks596","article-title":"Primer3\u2013new capabilities and interfaces","volume":"40","author":"Untergasser","year":"2012","journal-title":"Nucleic Acids Res"},{"key":"bib40","doi-asserted-by":"crossref","first-page":"16-22","DOI":"10.1073\/pnas.2235688100","article-title":"Electroporation and RNA interference in the rodent retina in vivo and in vitro","volume":"101","author":"Matsuda","year":"2004","journal-title":"Proc Natl Acad Sci USA"},{"key":"bib41","doi-asserted-by":"publisher","first-page":"12481-12486","DOI":"10.1073\/pnas.0605298103","article-title":"NF-kappaB-dependent induction of microRNA miR-146, an inhibitor targeted to signaling proteins of innate immune responses","volume":"103","author":"Taganov","year":"2006","journal-title":"Proc Natl Acad Sci USA"},{"key":"bib42","doi-asserted-by":"publisher","first-page":"e131610","DOI":"10.1172\/jci.insight.131610","article-title":"Using a barcoded AAV capsid library to select for clinically relevant gene therapy vectors","volume":"4","author":"Pekrun","year":"2019","journal-title":"JCI Insight"},{"key":"bib43","doi-asserted-by":"publisher","first-page":"373-383","DOI":"10.1038\/onc.2013.562","article-title":"Elafin drives poor outcome in high-grade serous ovarian cancers and basal-like breast tumors","volume":"34","author":"Labidi-Galy","year":"2015","journal-title":"Oncogene"},{"key":"bib44","doi-asserted-by":"publisher","first-page":"677","DOI":"10.1017\/S0952523805225166","article-title":"Independent visual threshold measurements in the two eyes of freely moving rats and mice using a virtual-reality optokinetic system","volume":"22","author":"Douglas","year":"2005","journal-title":"Vis Neurosci"},{"key":"bib45","doi-asserted-by":"publisher","first-page":"589-602","DOI":"10.1002\/(ISSN)1521-2254","article-title":"Mechanisms of AAV transduction in glaucoma-associated human trabecular meshwork cells","volume":"8","author":"Borr\u00e1s","year":"2006","journal-title":"J Gene Med"},{"key":"bib46","doi-asserted-by":"publisher","first-page":"1229-1236","DOI":"10.1016\/j.bbrc.2005.09.182","article-title":"Induction of IL-6 expression by mechanical stress in the trabecular meshwork","volume":"337","author":"Liton","year":"2005","journal-title":"Biochem Biophys Res Commun"},{"key":"bib47","doi-asserted-by":"publisher","first-page":"578","DOI":"10.3389\/fimmu.2014.00578","article-title":"MicroRNA-146a: a dominant, negative regulator of the innate immune response","volume":"5","author":"Saba","year":"2014","journal-title":"Front Immunol"},{"key":"bib48","first-page":"1041-1048","article-title":"The role of miR-146a in modulating TRAF6-induced inflammation during lupus nephritis","volume":"21","author":"Zheng","year":"2017","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"bib49","doi-asserted-by":"crossref","first-page":"4013487","DOI":"10.1155\/2016\/4013487","article-title":"MicroRNA-146a contributes to SCI recovery via regulating TRAF6 and IRAK1 expression","volume":"2016","author":"Wei","year":"2016","journal-title":"Biomed Res Int"},{"key":"bib50","doi-asserted-by":"publisher","first-page":"1369","DOI":"10.3390\/cancers11091369","article-title":"MicroRNA regulation of the autotaxin-lysophosphatidic acid signaling axis","volume":"11","author":"Murph","year":"2019","journal-title":"Cancers (Basel)"},{"key":"bib51","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1186\/1476-4598-9-71","article-title":"Autotaxin expression and its connection with the TNF-alpha-NF-kappaB axis in human hepatocellular carcinoma","volume":"9","author":"Wu","year":"2010","journal-title":"Mol Cancer"},{"key":"bib52","doi-asserted-by":"publisher","first-page":"165560","DOI":"10.1016\/j.bbadis.2019.165560","article-title":"Role of the autotaxin-lysophosphatidic acid axis in glaucoma, aqueous humor drainage and fibrogenic activity","volume":"1866","author":"Ho","year":"2020","journal-title":"Biochim Biophys Acta Mol Basis Dis"},{"key":"bib53","doi-asserted-by":"publisher","first-page":"628-634","DOI":"10.1093\/abbs\/gmx052","article-title":"miR-146a-5p acts as a negative regulator of TGF-\u00df signaling in skeletal muscle after acute contusion","volume":"49","author":"Sun","year":"2017","journal-title":"Acta Biochim Biophys Sin (Shanghai)"},{"key":"bib54","doi-asserted-by":"publisher","first-page":"335-338","DOI":"10.1016\/j.omtn.2017.04.016","article-title":"MicroRNA-146a-5p limits elevated TGF-\u00df signal during cell senescence","volume":"7","author":"Min","year":"2017","journal-title":"Mol Ther Nucleic Acids"},{"key":"bib55","doi-asserted-by":"publisher","first-page":"551","DOI":"10.1167\/iovs.08-2632","article-title":"Cochlin expression in anterior segment organ culture models after TGFbeta2 treatment","volume":"50","author":"Battacharya","year":"2009","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"bib56","doi-asserted-by":"publisher","first-page":"2067","DOI":"10.1167\/iovs.09-4567","article-title":"Adenoviral gene transfer of active human transforming growth factor-\u00df2 elevates intraocular pressure and reduces outflow facility in rodent eyes","volume":"51","author":"Shepard","year":"2010","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"bib57","doi-asserted-by":"publisher","first-page":"308","DOI":"10.1167\/iovs.09-3380","article-title":"Ocular gene transfer of active TGF-\u00df induces changes in anterior segment morphology and elevated IOP in rats","volume":"51","author":"Robertson","year":"2010","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"bib58","doi-asserted-by":"publisher","first-page":"680","DOI":"10.1136\/bjophthalmol-2011-301132","article-title":"The role of transforming growth factor \u00df in glaucoma and the therapeutic implications","volume":"97","author":"Prendes","year":"2013","journal-title":"Br J Ophthalmol"},{"key":"bib59","doi-asserted-by":"publisher","first-page":"173-178","DOI":"10.1001\/archophthalmol.2008.595","article-title":"Reduction of intraocular pressure with anecortave acetate in eyes with ocular steroid injection-related glaucoma","volume":"127","author":"Robin","year":"2009","journal-title":"Arch Ophthalmol"},{"key":"bib60","first-page":"3351-3364","article-title":"miR-146a regulates mechanotransduction and pressure-induced inflammation in small airway epithelium","volume":"26","author":"Huang","year":"2012","journal-title":"FASEB J"},{"key":"bib61","doi-asserted-by":"publisher","first-page":"451-463","DOI":"10.3892\/ijmm.2014.1808","article-title":"Role of miR-146a in human chondrocyte apoptosis in response to mechanical pressure injury in vitro","volume":"34","author":"Jin","year":"2014","journal-title":"Int J Mol Med"},{"key":"bib62","doi-asserted-by":"publisher","first-page":"1896-1903","DOI":"10.1002\/pros.23068","article-title":"Hsa-miR-146a-5p modulates androgen-independent prostate cancer cells apoptosis by targeting ROCK1","volume":"75","author":"Xu","year":"2015","journal-title":"Prostate"},{"key":"bib63","doi-asserted-by":"publisher","first-page":"26697","DOI":"10.1038\/srep26697","article-title":"MicroRNA-146a suppresses ROCK1 allowing hyperphosphorylation of tau in Alzheimer's disease","volume":"6","author":"Wang","year":"2016","journal-title":"Sci Rep"},{"key":"bib64","doi-asserted-by":"publisher","first-page":"109-126","DOI":"10.1007\/s10384-018-0566-9","article-title":"Impact of the clinical use of ROCK inhibitor on the pathogenesis and treatment of glaucoma","volume":"62","author":"Honjo","year":"2018","journal-title":"Jpn J Ophthalmol"},{"key":"bib65","doi-asserted-by":"publisher","first-page":"97-106","DOI":"10.1038\/sj.mt.6300318","article-title":"Durable, safe, multi-gene lentiviral vector expression in feline trabecular meshwork","volume":"16","author":"Khare","year":"2008","journal-title":"Mol Ther"}],"container-title":["Translational Vision Science &amp; Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/tvst.arvojournals.org\/article.aspx?articleid=2776460","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,13]],"date-time":"2021-07-13T14:31:44Z","timestamp":1626186704000},"score":1,"resource":{"primary":{"URL":"https:\/\/tvst.arvojournals.org\/article.aspx?articleid=2776460"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,13]]},"references-count":65,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2021,7,1]]}},"URL":"https:\/\/doi.org\/10.1167\/tvst.10.8.14","relation":{},"ISSN":["2164-2591"],"issn-type":[{"value":"2164-2591","type":"print"}],"subject":[],"published":{"date-parts":[[2021,7,13]]}}}