{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T15:37:56Z","timestamp":1763480276702},"reference-count":29,"publisher":"Association for Research in Vision and Ophthalmology (ARVO)","issue":"3","license":[{"start":{"date-parts":[[2019,6,4]],"date-time":"2019-06-04T00:00:00Z","timestamp":1559606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Trans. Vis. Sci. Tech."],"published-print":{"date-parts":[[2019,6,4]]},"DOI":"10.1167\/tvst.8.3.33","type":"journal-article","created":{"date-parts":[[2019,6,4]],"date-time":"2019-06-04T16:51:05Z","timestamp":1559667065000},"page":"33","source":"Crossref","is-referenced-by-count":5,"title":["Analysis of Melanin Structure and Biochemical Composition in Conjunctival Melanocytic Lesions Using Pump\u2013Probe Microscopy"],"prefix":"10.1167","volume":"8","author":[{"given":"Francisco E.","family":"Robles","sequence":"first","affiliation":[{"name":"Department of Chemistry, Duke University, Durham, NC, USA"},{"name":"Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanghamitra","family":"Deb","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Duke University, Durham, NC, USA"},{"name":"Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lejla","family":"Vajzovic","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Duke University Medical Center, Durham, NC, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gargi K.","family":"Vora","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Duke University Medical Center, Durham, NC, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prithvi","family":"Mruthyunjaya","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Duke University Medical Center, Durham, NC, USA"},{"name":"Department of Ophthalmology, Stanford University Medical Center, Palo Alto, CA, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Warren S.","family":"Warren","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Duke University, Durham, NC, USA"},{"name":"Department of Radiology, Duke University Medical Center, Durham, NC, USA"},{"name":"Duke University, Departments of Physics, Durham, NC, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"27","reference":[{"key":"i2164-2591-8-3-33-b01","doi-asserted-by":"crossref","unstructured":"Damato B, Coupland SE. Conjunctival melanoma and melanosis: a reappraisal of terminology, classification and staging. Clin Exp Ophthalmol. 2008; 36: 786\u2013795.","DOI":"10.1111\/j.1442-9071.2008.01888.x"},{"key":"i2164-2591-8-3-33-b02","doi-asserted-by":"crossref","unstructured":"Zembowicz A, Mandal RV, Choopong P. Melanocytic lesions of the conjunctiva. Arch Pathol Lab Med. 2010; 134; 1785\u20131792.","DOI":"10.5858\/2009-0522-RAR.1"},{"key":"i2164-2591-8-3-33-b03","doi-asserted-by":"crossref","unstructured":"McCartney ACE. Pathology of ocular melanomas. Br Med Bull. 1995; 51: 678\u2013693.","DOI":"10.1093\/oxfordjournals.bmb.a072986"},{"key":"i2164-2591-8-3-33-b04","doi-asserted-by":"crossref","unstructured":"Glusac EJ. The melanoma \u2018epidemic', a dermatopathologist's perspective. J Cutan Pathol. 2011; 38; 264\u2013267.","DOI":"10.1111\/j.1600-0560.2010.01660.x"},{"key":"i2164-2591-8-3-33-b05","doi-asserted-by":"crossref","unstructured":"Yu GP, Hu DN, McCormick S, Finger PT. Conjunctival melanoma: is it increasing in the United States? Am J Ophthalmol. 2003; 135; 800\u2013806.","DOI":"10.1016\/S0002-9394(02)02288-2"},{"key":"i2164-2591-8-3-33-b06","doi-asserted-by":"crossref","unstructured":"Shields CL, Markowitz JS, Belinsky I, et al. Conjunctival Melanoma: Outcomes Based On Tumor Origin In 382 Consecutive Cases. Ophthalmology. 2011; 118; 389\u2013395.e2.","DOI":"10.1016\/j.ophtha.2010.06.021"},{"key":"i2164-2591-8-3-33-b07","unstructured":"Shields JA, Shields CL, Mashayekhi A, et al. Primary acquired melanosis of the conjunctiva: experience with 311 eyes. Trans Am Ophthalmol Soc. 2007; 105: 61\u201371, discussion 71\u201372."},{"key":"i2164-2591-8-3-33-b08","doi-asserted-by":"crossref","unstructured":"Vora GK, Demirci H, Marr B, Mruthyunjaya P. Advances in the management of conjunctival melanoma. Surv Ophthalmol. 62, 26\u201342 (2017).","DOI":"10.1016\/j.survophthal.2016.06.001"},{"key":"i2164-2591-8-3-33-b09","doi-asserted-by":"crossref","unstructured":"Zembowicz A, Mandal RV, Choopong P. Melanocytic lesions of the conjunctiva. Arch Pathol Lab Med. 2010; 134: 1785\u20131792.","DOI":"10.5858\/2009-0522-RAR.1"},{"key":"i2164-2591-8-3-33-b10","doi-asserted-by":"crossref","unstructured":"Mudhar HS. Melanocytic lesions of the conjunctiva. Diagn Histopathol. 2015; 21: 1\u201310.","DOI":"10.1016\/j.mpdhp.2014.11.004"},{"key":"i2164-2591-8-3-33-b11","unstructured":"Amin MB, Edge SB, Greene FL, et al. Malignant melanoma of the conjunctiva. In: AJCC Cancer Staging Manual, 8th ed. New York, NY: Springer; 2017."},{"key":"i2164-2591-8-3-33-b12","doi-asserted-by":"crossref","unstructured":"Messmer EM, Mackert MJ, Zapp DM, Kampik A. In vivo confocal microscopy of pigmented conjunctival tumors. Graefe's Arch Clin Exp Ophthalmol. 2006; 244: 1437\u20131445.","DOI":"10.1007\/s00417-006-0284-8"},{"key":"i2164-2591-8-3-33-b13","doi-asserted-by":"crossref","unstructured":"Nanji AA, Sayyad FE, Galor A, Dubovy S, Karp CL. High-resolution optical coherence tomography as an adjunctive tool in the diagnosis of corneal and conjunctival pathology. Ocul Surf. 2015; 13: 226\u2013235.","DOI":"10.1016\/j.jtos.2015.02.001"},{"key":"i2164-2591-8-3-33-b14","doi-asserted-by":"crossref","unstructured":"Ho VH, Prager TC, Diwan H, Prieto V, Esmaeli B. Ultrasound biomicroscopy for estimation of tumor thickness for conjunctival melanoma. J Clin Ultrasound. 2007; 35: 533\u2013537.","DOI":"10.1002\/jcu.20343"},{"key":"i2164-2591-8-3-33-b15","doi-asserted-by":"crossref","unstructured":"Matthews TE, Piletic IR, Selim MA, Simpson MJ, Warren WS. Pump-probe imaging differentiates melanoma from melanocytic nevi. Sci Transl Med. 2011; 23: 7tra15.","DOI":"10.1126\/scitranslmed.3001604"},{"key":"i2164-2591-8-3-33-b16","doi-asserted-by":"crossref","unstructured":"Robles FE, Deb S, Wilson JW, et al. Pump-probe imaging of pigmented cutaneous melanoma primary lesions gives insight into metastatic potential. Biomed Opt Exp. 2015; 6: 3631\u20133645.","DOI":"10.1364\/BOE.6.003631"},{"key":"i2164-2591-8-3-33-b17","doi-asserted-by":"crossref","unstructured":"Robles FE, Deb S, Fischer MC, Warren WS, Selim MA. Label-free imaging of female genital tract melanocytic lesions with pump-probe microscopy. J Low Genit Tract Dis. 2017; 21: 137\u2013144.","DOI":"10.1097\/LGT.0000000000000290"},{"key":"i2164-2591-8-3-33-b18","doi-asserted-by":"crossref","unstructured":"Simpson MJ, Wilson JW, Robles FE, et al. Near-infrared excited state dynamics of melanins: the effects of iron content, photo-damage, chemical oxidation, and aggregate size. J Phys Chem A. 2014; 118: 993\u20131003.","DOI":"10.1021\/jp4107475"},{"key":"i2164-2591-8-3-33-b19","doi-asserted-by":"crossref","unstructured":"Robles FE, Wilson JW, Warren WS. Quantifying melanin spatial distribution using pump-probe microscopy and a 2-D morphological autocorrelation transformation for melanoma diagnosis. J Biomed Opt. 2013; 18: 120502.","DOI":"10.1117\/1.JBO.18.12.120502"},{"key":"i2164-2591-8-3-33-b20","doi-asserted-by":"crossref","unstructured":"Wilson JW, Vajzovic L, Robles FE, Cummings TJ, Mruthyunjaya P, Warren WS. Imaging microscopic pigment chemistry in conjunctival melanocytic lesions using pump-probe laser microscopy. Invest Ophthalmol Vis Sci. 2013; 54: 6867\u20136876.","DOI":"10.1167\/iovs.13-12432"},{"key":"i2164-2591-8-3-33-b21","doi-asserted-by":"crossref","unstructured":"Fu D, Ye T, Matthews TE, Yurtsever G, Warren WS. Two-color, two-photon, and excited-state absorption microscopy. J Biomed Opt. 2007; 12: 054004.","DOI":"10.1117\/1.2780173"},{"key":"i2164-2591-8-3-33-b22","doi-asserted-by":"crossref","unstructured":"Fischer MC, Wilson JW, Robles FE, Warren WS. Invited review article: pump-probe microscopy. Rev Sci Instrum. 2016; 87: 031101.","DOI":"10.1063\/1.4943211"},{"key":"i2164-2591-8-3-33-b23","doi-asserted-by":"crossref","unstructured":"Thompson A, Robles FE, Wilson JW, Deb S, Calderbank R, Warren WS. Dual-wavelength pump-probe microscopy analysis of melanin composition. Sci Rep. 2016; 6: 1\u201310.","DOI":"10.1038\/srep36871"},{"key":"i2164-2591-8-3-33-b24","doi-asserted-by":"crossref","unstructured":"Robles FE, Wilson JW, Fischer MC, Warren WS. Phasor analysis for nonlinear pump-probe microscopy. Opt Exp. 2012; 20: 17082.","DOI":"10.1364\/OE.20.017082"},{"key":"i2164-2591-8-3-33-b25","doi-asserted-by":"crossref","unstructured":"Ju KY, Fischer MC, Warren WS. Understanding the role of aggregation in the broad absorption bands of eumelanin. ACS Nano. 2018; 12: 12050\u201312061.","DOI":"10.1021\/acsnano.8b04905"},{"key":"i2164-2591-8-3-33-b26","doi-asserted-by":"crossref","unstructured":"Jay B. Naevi and melanomata of the conjunctiva. Br J Ophthalmol. 1965; 49: 169\u2013204.","DOI":"10.1136\/bjo.49.4.169"},{"key":"i2164-2591-8-3-33-b27","doi-asserted-by":"crossref","unstructured":"Simpson MJ, Glass KE, Wilson JW, Wilby PR, Simon JD, Warren WS. Pump\u2013probe microscopic imaging of Jurassic-aged eumelanin. J Phys Chem Lett. 2013; 4: 1924\u20131927.","DOI":"10.1021\/jz4008036"},{"key":"i2164-2591-8-3-33-b28","doi-asserted-by":"crossref","unstructured":"Matthews TE, Wilson JW, Degan S, et al. In vivo and ex vivo epi-mode pump-probe imaging of melanin and microvasculature. Biomed Opt Exp. 2011; 2: 1576\u20131583.","DOI":"10.1364\/BOE.2.001576"},{"key":"i2164-2591-8-3-33-b29","doi-asserted-by":"crossref","unstructured":"Wilson JW, Degan S, Gainey CS, et al. Comparing in vivo pump\u2013probe and multiphoton fluorescence microscopy of melanoma and pigmented lesions. J Biomed Opt. 2015; 20: 051012.","DOI":"10.1117\/1.JBO.20.5.051012"}],"container-title":["Translational Vision Science &amp; Technology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/tvst.arvojournals.org\/article.aspx?articleid=2735402","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,19]],"date-time":"2022-09-19T12:37:01Z","timestamp":1663591021000},"score":1,"resource":{"primary":{"URL":"http:\/\/tvst.arvojournals.org\/article.aspx?doi=10.1167\/tvst.8.3.33"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,6,4]]},"references-count":29,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019,5,1]]}},"URL":"https:\/\/doi.org\/10.1167\/tvst.8.3.33","relation":{},"ISSN":["2164-2591"],"issn-type":[{"value":"2164-2591","type":"print"}],"subject":[],"published":{"date-parts":[[2019,6,4]]}}}