{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T01:21:45Z","timestamp":1792632105171,"version":"4.5.0"},"reference-count":33,"publisher":"Association for Research in Vision and Ophthalmology (ARVO)","issue":"10","license":[{"start":{"date-parts":[[2020,9,23]],"date-time":"2020-09-23T00:00:00Z","timestamp":1600819200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Trans. Vis. Sci. Tech."],"published-print":{"date-parts":[[2020,9,23]]},"DOI":"10.1167\/tvst.9.10.23","type":"journal-article","created":{"date-parts":[[2020,9,23]],"date-time":"2020-09-23T15:31:14Z","timestamp":1600875074000},"page":"23","source":"Crossref","is-referenced-by-count":5,"title":["Quantifying Sample Collection and Processing Impacts on Fiber-Based Tear Fluid Chemical Analysis"],"prefix":"10.1167","volume":"9","author":[{"given":"Anis","family":"Barmada","sequence":"first","affiliation":[{"name":"Department of Chemistry, University of Illinois at Chicago, Chicago, Illinois, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott A.","family":"Shippy","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Illinois at Chicago, Chicago, Illinois, United States"},{"name":"Laboratory of Integrative Neuroscience, University of Illinois at Chicago, Chicago, Illinois, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"27","reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"923","DOI":"10.1038\/sj.eye.6700566","article-title":"Tears in health and disease","volume":"17","author":"Tiffany","year":"2003","journal-title":"Eye"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"527","DOI":"10.1016\/j.preteyeres.2012.06.002","article-title":"Tear analysis in ocular surface diseases","volume":"31","author":"Zhou","year":"2012","journal-title":"Prog Retin Eye Res"},{"key":"bib3","doi-asserted-by":"crossref","DOI":"10.1038\/s41433-020-0930-0","article-title":"Tear analysis as the next routine body fluid test","author":"Barmada","year":"2020","journal-title":"Eye"},{"key":"bib4","doi-asserted-by":"publisher","first-page":"799","DOI":"10.1016\/j.ajo.2010.11.003","article-title":"Amino acid profiles in human tear fluids analyzed by high-performance liquid chromatography and electrospray ionization tandem mass spectrometry","volume":"151","author":"Nakatsukasa","year":"2011","journal-title":"Am J Ophthalmol"},{"key":"bib5","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1016\/j.exer.2013.07.015","article-title":"Tears as a source of biomarkers for ocular and systemic diseases","volume":"117","author":"von Thun Und Hohenstein-Blaul","year":"2013","journal-title":"Exp Eye Res"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1194\/jlr.M044826","article-title":"Extensive characterization of human tear fluid collected using different techniques unravels the presence of novel lipid amphiphiles","volume":"55","author":"Lam","year":"2014","journal-title":"J Lipid Res"},{"key":"bib7","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1167\/tvst.7.6.22","article-title":"Combined targeted analysis of metabolites and proteins in tear fluid with regard to clinical applications","volume":"7","author":"Dammeier","year":"2018","journal-title":"Transl Vis Sci Technol"},{"key":"bib8","doi-asserted-by":"publisher","first-page":"4876","DOI":"10.1021\/pr2004874","article-title":"Characterization of the human tear metabolome by LC-MS\/MS","volume":"10","author":"Chen","year":"2011","journal-title":"J Proteome Res"},{"key":"bib9","doi-asserted-by":"publisher","first-page":"5052","DOI":"10.1167\/iovs.11-9022","article-title":"iTRAQ quantitative proteomics in the analysis of tears in dry eye patients","volume":"53","author":"Srinivasan","year":"2012","journal-title":"Investig Ophthalmol Vis Sci"},{"key":"bib10","doi-asserted-by":"publisher","first-page":"e0200702","DOI":"10.1371\/journal.pone.0200702","article-title":"The use of in-strip digestion for fast proteomic analysis on tear fluid from dry eye patients","volume":"13","author":"Huang","year":"2018","journal-title":"PLoS One"},{"key":"bib11","doi-asserted-by":"publisher","first-page":"3877","DOI":"10.1016\/j.jprot.2012.04.053","article-title":"In-depth analysis of the human tear proteome","volume":"75","author":"Zhou","year":"2012","journal-title":"J Proteom"},{"key":"bib12","first-page":"2055","article-title":"Proteomic analysis of the tear film in patients with keratoconus","volume":"16","author":"Lema","year":"2010","journal-title":"Mol Vis"},{"key":"bib13","doi-asserted-by":"publisher","first-page":"3420","DOI":"10.1002\/elps.201000271","article-title":"Two dimensional electrophoretic analysis of human tears: collection method in dry eye syndrome","volume":"31","author":"Saijyothi","year":"2010","journal-title":"Electrophoresis"},{"key":"bib14","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1007\/s00216-018-1488-6","article-title":"Thread-based assay for quantitative small molecule analysis of mice tear fluid by capillary electrophoresis","volume":"411","author":"Barmada","year":"2019","journal-title":"Anal Bioanal Chem"},{"key":"bib15","doi-asserted-by":"publisher","first-page":"5309","DOI":"10.1007\/s00216-016-9624-7","article-title":"Threads for tear film collection and support in quantitative amino acid analysis","volume":"408","author":"Avilov","year":"2016","journal-title":"Anal Bioanal Chem"},{"key":"bib16","doi-asserted-by":"publisher","first-page":"12034","DOI":"10.1038\/ncomms12034","article-title":"PACAP suppresses dry eye signs by stimulating tear secretion","volume":"7","author":"Nakamachi","year":"2016","journal-title":"Nat Commun"},{"key":"bib17","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1111\/vop.2013.16.issue-4","article-title":"Correlation between corneal sensitivity and quantity of reflex tearing in cows, horses, goats, sheep, dogs, cats, rabbits, and guinea pigs","volume":"16","author":"Wieser","year":"2013","journal-title":"Vet Ophthalmol"},{"key":"bib18","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1177\/0004563217695843","article-title":"Evaluation of commonly used tear sampling methods and their relevance in subsequent biochemical analysis","volume":"54","author":"Rentka","year":"2017","journal-title":"Ann Clin Biochem"},{"key":"bib19","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1016\/j.aanat.2012.10.001","article-title":"Schirmer strip vs. capillary tube method: non-invasive methods of obtaining proteins from tear fluid","volume":"195","author":"Posa","year":"2013","journal-title":"Ann Anat"},{"key":"bib20","doi-asserted-by":"publisher","first-page":"722","DOI":"10.3109\/02713683.2013.777967","article-title":"Evaluation of the frequency of ophthalmic solution application: washout effects of topical saline application on tear components","volume":"38","author":"Shigeyasu","year":"2013","journal-title":"Curr Eye Res"},{"key":"bib21","doi-asserted-by":"publisher","first-page":"1391","DOI":"10.1097\/OPX.0000000000000411","article-title":"Comparison of tear lipid profile among basal, reflex, and flush tear samples","volume":"91","author":"Rohit","year":"2014","journal-title":"Optom Vis Sci"},{"key":"bib22","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1016\/j.clae.2013.11.011","article-title":"Influence of eye position on the Schirmer tear test","volume":"37","author":"Bitton","year":"2014","journal-title":"Contact Lens Anterior Eye"},{"key":"bib23","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1016\/0014-4835(86)90026-6","article-title":"Acute reversible cataract induced by xylazine and by ketamine-xylazine anesthesia in rats and mice","volume":"42","author":"Calderone","year":"1986","journal-title":"Exp Eye Res"},{"key":"bib24","doi-asserted-by":"publisher","first-page":"7725","DOI":"10.1167\/iovs.11-7266","article-title":"Proinflammatory cytokine profiling of tears from dry eye patients by means of antibody microarrays","volume":"52","author":"Boehm","year":"2011","journal-title":"Investig Ophthalmol Vis Sci"},{"key":"bib25","first-page":"3130","article-title":"Water-soluble antioxidants in human tears: effect of the collection method","volume":"42","author":"Choy","year":"2001","journal-title":"Investig Ophthalmol Vis Sci"},{"key":"bib26","doi-asserted-by":"publisher","first-page":"853","DOI":"10.1007\/s00417-009-1275-3","article-title":"Quantification of tear proteins by SDS-PAGE with an internal standard protein: a new method with special reference to small volume tears","volume":"248","author":"Li","year":"2010","journal-title":"Graefes Arch Clin Exp Ophthalmol"},{"key":"bib27","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/j.clae.2017.09.012","article-title":"The potential influence of Schirmer strip variables on dry eye disease characterisation, and on tear collection and analysis","volume":"41","author":"Garc\u00eda-Porta","year":"2018","journal-title":"Contact Lens Anterior Eye"},{"key":"bib28","doi-asserted-by":"publisher","first-page":"5088","DOI":"10.1039\/c2an35821b","article-title":"Post-collection processing of Schirmer strip-collected human tear fluid impacts protein content","volume":"137","author":"Denisin","year":"2012","journal-title":"Analyst"},{"key":"bib29","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1186\/s12917-018-1390-7","article-title":"Effect of tear collection on lacrimal total protein content in dogs and cats: a comparison between Schirmer strips and ophthalmic sponges","volume":"14","author":"Sebbag","year":"2018","journal-title":"BMC Vet Res"},{"key":"bib30","doi-asserted-by":"publisher","first-page":"4495","DOI":"10.1021\/acs.analchem.7b04840","article-title":"Impact of sampling and cellular separation on amino acid determinations in Drosophila hemolymph","volume":"90","author":"Cabay","year":"2018","journal-title":"Anal Chem"},{"key":"bib31","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1136\/bjo.68.8.549","article-title":"Dynamics of ocular surface pH","volume":"68","author":"Coles","year":"1984","journal-title":"B J Ophthalmol"},{"key":"bib32","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1007\/s00216-008-2304-5","article-title":"A high-efficiency sample introduction system for capillary electrophoresis analysis of amino acids from dynamic samples and static dialyzed human vitreous samples","volume":"392","author":"Patterson","year":"2008","journal-title":"Anal Bioanal Chem"},{"key":"bib33","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/j.colsurfa.2016.08.027","article-title":"Human tear-production rate from closed-eye Schirmer-strip capillary dynamics","volume":"521","author":"Telles","year":"2017","journal-title":"Colloids Surf A Physicochem Eng Asp"}],"container-title":["Translational Vision Science & Technology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/tvst.arvojournals.org\/article.aspx?articleid=2770827","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,3]],"date-time":"2021-04-03T18:20:39Z","timestamp":1617474039000},"score":1,"resource":{"primary":{"URL":"https:\/\/tvst.arvojournals.org\/article.aspx?articleid=2770827"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,23]]},"references-count":33,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2020,9,1]]}},"URL":"https:\/\/doi.org\/10.1167\/tvst.9.10.23","relation":{},"ISSN":["2164-2591"],"issn-type":[{"value":"2164-2591","type":"print"}],"subject":[],"published":{"date-parts":[[2020,9,23]]}}}