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To address these limitations, we developed a translational model of CRS-1, cardiac arrest and cardiopulmonary resuscitation (CA\/CPR), and now report findings from nanoscale mass spectrometry proteomic exploration of glomerular filtrate 2 hours after CA\/CPR or sham procedure. Filtrate acquisition was confirmed by imaging, molecular weight and charge distribution, and exclusion of protein specific to surrounding cells. Filtration of proteins specific to the heart was detected following CA\/CPR and confirmed with mass spectrometry performed using urine collections from mice with deficient tubular endocytosis. Cardiac LIM protein was a CA\/CPR-specific filtrate component. Cardiac arrest induced plasma release of cardiac LIM protein in mice and critically ill human cardiac arrest survivors, and administration of recombinant cardiac LIM protein to mice altered renal function. These findings demonstrate that glomerular filtrate is accessible to nanoscale proteomics and elucidate the population of proteins filtered 2 hours after CA\/CPR. The identification of cardiac-specific proteins in renal filtrate suggests a novel signaling mechanism in CRS-1. We expect these findings to advance understanding of CRS-1.<\/jats:p>","DOI":"10.1172\/jci.insight.122130","type":"journal-article","created":{"date-parts":[[2019,2,20]],"date-time":"2019-02-20T11:01:38Z","timestamp":1550660498000},"source":"Crossref","is-referenced-by-count":14,"title":["Glomerular filtrate proteins in acute cardiorenal syndrome"],"prefix":"10.1172","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0229-1919","authenticated-orcid":true,"given":"Rumie","family":"Wakasaki","sequence":"first","affiliation":[{"name":"Anesthesiology & Perioperative Medicine, Oregon Health & Science University, Portland, Oregon, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuyuki","family":"Matsushita","sequence":"additional","affiliation":[{"name":"Anesthesiology & Perioperative Medicine, Oregon Health & Science University, Portland, Oregon, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kirsti","family":"Golgotiu","sequence":"additional","affiliation":[{"name":"Anesthesiology & Perioperative Medicine, Oregon Health & Science University, Portland, Oregon, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharon","family":"Anderson","sequence":"additional","affiliation":[{"name":"Operative Care Division and Research and Development Division, Portland Veterans Affairs Medical Center, Portland, Oregon, USA."},{"name":"Division of Nephrology and Hypertension, Oregon Health & Science University, Portland, Oregon, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahaba B.","family":"Eiwaz","sequence":"additional","affiliation":[{"name":"Anesthesiology & Perioperative Medicine, Oregon Health & Science University, Portland, Oregon, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0253-6859","authenticated-orcid":true,"given":"Daniel J.","family":"Orton","sequence":"additional","affiliation":[{"name":"Pacific Northwest National Laboratory, Environmental and Biological Services Division, Richland, Washington, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang Jun","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, College of Physicians and Surgeons of Columbia University, New York, New York, USA."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. 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