{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T19:34:06Z","timestamp":1793216046131,"version":"4.5.0"},"reference-count":63,"publisher":"American Society for Clinical Investigation","issue":"18","funder":[{"name":"Secretar\u00eda de Ciencia y Tecnolog\u00eda, Universidad Nacional de C\u00f3rdoba","award":["30720150101066CB"],"award-info":[{"award-number":["30720150101066CB"]}]},{"name":"Fondo para la Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica from Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","award":["PICT 523 2013-2885"],"award-info":[{"award-number":["PICT 523 2013-2885"]}]},{"name":"Fondo para la Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica from Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","award":["PICT 2015-1130"],"award-info":[{"award-number":["PICT 2015-1130"]}]},{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"crossref","award":["PIP 11220120100620"],"award-info":[{"award-number":["PIP 11220120100620"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,9,19]]},"abstract":"<jats:p>\n                    Chagas disease is a lifelong pathology resulting from\n                    <jats:italic>Trypanosoma cruzi<\/jats:italic>\n                    infection. It represents one of the most frequent causes of heart failure and sudden death in Latin America. Herein, we provide evidence that aerobic glycolytic pathway activation in monocytes drives nitric oxide (NO) production, triggering tyrosine nitration (TN) on CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells and dysfunction in patients with chronic Chagas disease. Monocytes from patients exhibited a higher frequency of hypoxia-inducible factor 1\u03b1 and increased expression of its target genes\/proteins. Nonclassical monocytes are expanded in patients\u2019 peripheral blood and represent an important source of NO. Monocytes entail CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cell surface nitration because both the frequency of nonclassical monocytes and that of NO-producing monocytes positively correlated with the percentage of TN\n                    <jats:sup>+<\/jats:sup>\n                    lymphocytes. Inhibition of glycolysis in in vitro\u2013infected peripheral blood mononuclear cells decreased the inflammatory properties of monocytes\/macrophages, diminishing the frequency of IL-1\u03b2\u2013 and NO-producing cells. In agreement, glycolysis inhibition reduced the percentage of TN\n                    <jats:sup>+<\/jats:sup>\n                    CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells, improving their functionality. Altogether, these results clearly show that glycolysis governs oxidative stress on monocytes and modulates monocyte\u2013T cell interplay in human chronic Chagas disease. Understanding the pathological immune mechanisms that sustain an inflammatory environment in human pathology is key to designing improved therapies.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.123490","type":"journal-article","created":{"date-parts":[[2019,9,3]],"date-time":"2019-09-03T12:14:17Z","timestamp":1567512857000},"source":"Crossref","is-referenced-by-count":18,"title":["Monocyte glycolysis determines CD8+ T cell functionality in human Chagas disease"],"prefix":"10.1172","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2075-8826","authenticated-orcid":true,"given":"Liliana Mar\u00eda","family":"Sanmarco","sequence":"first","affiliation":[{"name":"Universidad Nacional de C\u00f3rdoba, Facultad de Ciencias Qu\u00edmicas, Departamento de Bioqu\u00edmica Cl\u00ednica, C\u00f3rdoba, Argentina."},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y Tecnol\u00f3gicas (CONICET), Centro de Investigaciones en Bioqu\u00edmica Cl\u00ednica e 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