{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T20:35:35Z","timestamp":1793046935912,"version":"4.5.0"},"reference-count":31,"publisher":"American Society for Clinical Investigation","issue":"3","funder":[{"DOI":"10.13039\/100000052","name":"NIH Office of the Director","doi-asserted-by":"crossref","award":["DP2HL142045"],"award-info":[{"award-number":["DP2HL142045"]}],"id":[{"id":"10.13039\/100000052","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000052","name":"NIH Office of the Director","doi-asserted-by":"crossref","award":["OT2OD023848"],"award-info":[{"award-number":["OT2OD023848"]}],"id":[{"id":"10.13039\/100000052","id-type":"DOI","asserted-by":"crossref"}]},{"name":"NIH\/NHLBI","award":["HL125730"],"award-info":[{"award-number":["HL125730"]}]},{"name":"AIBA WORKS","award":["basic medical research fellowship"],"award-info":[{"award-number":["basic medical research fellowship"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,2,13]]},"abstract":"<jats:p>Chronic sympathoexcitation is implicated in ventricular arrhythmogenesis (VAs) following myocardial infarction (MI), but the critical neural pathways involved are not well understood. Cardiac adrenergic function is partly regulated by sympathetic afferent reflexes, transduced by spinal afferent fibers expressing the transient receptor potential cation subfamily V member 1 (TRPV1) channel. The role of chronic TRPV1 afferent signaling in VAs is not known. We hypothesized that persistent TRPV1 afferent neurotransmission promotes VAs after MI. Using epicardial resiniferatoxin (RTX) to deplete cardiac TRPV1\u2013expressing fibers, we dissected the role of this neural circuit in VAs after chronic MI in a porcine model. We examined the underlying mechanisms using molecular approaches, IHC, in vitro and in vivo cardiac electrophysiology, and simultaneous cardioneural mapping. Epicardial RTX depleted cardiac TRPV1 afferent fibers and abolished functional responses to TRPV1 agonists. Ventricular tachycardia\/fibrillation (VT\/VF) was readily inducible in MI subjects by programmed electrical stimulation or cesium chloride administration; however, TRPV1 afferent depletion prevented VT\/VF induced by either method. Mechanistically, TRPV1 afferent depletion did not alter cardiomyocyte action potentials and calcium transients, the expression of ion channels, or calcium handling proteins. However, it attenuated fibrosis and mitigated electrical instability in the scar border zone. In vivo recordings of cardiovascular-related stellate ganglion neurons (SGNs) revealed that MI enhances SGN function and disrupts integrated neural processing. Depleting TRPV1 afferents normalized these processes. Taken together, these data indicate that, after MI, TRPV1 afferent\u2013induced adrenergic dysfunction promotes fibrosis and adverse cardiac remodeling, and it worsens border zone electrical heterogeneity, resulting in electrically unstable ventricular myocardium. We propose targeting TRPV1-expressing afferent to reduce VT\/VF following MI.<\/jats:p>","DOI":"10.1172\/jci.insight.124477","type":"journal-article","created":{"date-parts":[[2019,12,17]],"date-time":"2019-12-17T12:00:54Z","timestamp":1576584054000},"source":"Crossref","is-referenced-by-count":85,"title":["Cardiac TRPV1 afferent signaling promotes arrhythmogenic ventricular remodeling after myocardial infarction"],"prefix":"10.1172","volume":"5","author":[{"given":"Koji","family":"Yoshie","sequence":"first","affiliation":[{"name":"Cardiac Arrhythmia Center and Neurocardiology Research Program,"},{"name":"Division of Cardiology, Department of Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9790-0408","authenticated-orcid":true,"given":"Pradeep S.","family":"Rajendran","sequence":"additional","affiliation":[{"name":"Cardiac Arrhythmia Center and Neurocardiology Research Program,"},{"name":"Division of Cardiology, Department of Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louis","family":"Massoud","sequence":"additional","affiliation":[{"name":"Cardiac Arrhythmia Center and Neurocardiology Research Program,"},{"name":"Division of Cardiology, Department of Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janki","family":"Mistry","sequence":"additional","affiliation":[{"name":"Cardiac Arrhythmia Center and Neurocardiology Research Program,"},{"name":"Division of Cardiology, Department of Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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