{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:07:22Z","timestamp":1790788042412,"version":"4.1.0"},"reference-count":39,"publisher":"American Society for Clinical Investigation","issue":"21","funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["UC4-DK104196"],"award-info":[{"award-number":["UC4-DK104196"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,11,1]]},"abstract":"<jats:p>\n                    Islet transplantation is an effective therapy for achieving and maintaining normoglycemia in patients with type 1 diabetes mellitus. However, the supply of transplantable human islets is limited. Upon removal from the pancreas, islets rapidly disintegrate and lose function, resulting in a short interval for studies of islet biology and pretransplantation assessment. Here, we developed a biomimetic platform that can sustain human islet physiology for a prolonged period ex vivo. Our approach involved the creation of a multichannel perifusion system to monitor dynamic insulin secretion and intracellular calcium flux simultaneously, enabling the systematic evaluation of glucose-stimulated insulin secretion under multiple conditions. Using this tool, we developed a nanofibrillar cellulose hydrogel\u2013based\n                    <jats:italic>islet-preserving platform<\/jats:italic>\n                    (\n                    <jats:italic>iPreP<\/jats:italic>\n                    ) that can preserve islet viability, morphology, and function for nearly 12 weeks ex vivo, and with the ability to ameliorate glucose levels upon transplantation into diabetic hosts. Our platform has potential applications in the prolonged maintenance of human islets, providing an expanded time window for pretransplantation assessment and islet studies.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.124644","type":"journal-article","created":{"date-parts":[[2019,10,31]],"date-time":"2019-10-31T11:01:33Z","timestamp":1572519693000},"source":"Crossref","is-referenced-by-count":8,"title":["iPreP is a three-dimensional nanofibrillar cellulose hydrogel platform for long-term ex vivo preservation of human islets"],"prefix":"10.1172","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4367-5724","authenticated-orcid":true,"given":"Yi-Ju","family":"Chen","sequence":"first","affiliation":[{"name":"Gastroenterology Division, Department of Medicine,"},{"name":"Department of Cell and Developmental Biology, and"},{"name":"Abramson Family Cancer Research Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA."},{"name":"Genetic Resource Science, The Jackson Laboratory, Bar Harbor, Maine, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taiji","family":"Yamazoe","sequence":"additional","affiliation":[{"name":"Gastroenterology Division, Department of Medicine,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karla F.","family":"Leavens","sequence":"additional","affiliation":[{"name":"Center for Cellular and Molecular Therapeutics, Department of Pathology and Laboratory Medicine,"},{"name":"Division of Endocrinology, Department of Pediatrics, Children\u2019s Hospital of Philadelphia, Philadelphia, Pennsylvania, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabian L.","family":"Cardenas-Diaz","sequence":"additional","affiliation":[{"name":"Center for Cellular and Molecular Therapeutics, Department of Pathology and Laboratory Medicine,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1612-3497","authenticated-orcid":true,"given":"Andrei","family":"Georgescu","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, School of Engineering and Applied Sciences, University of Pennsylvania, Philadelphia, Pennsylvania, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5718-6884","authenticated-orcid":true,"given":"Dongeun","family":"Huh","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, School of Engineering and Applied Sciences, University of Pennsylvania, Philadelphia, Pennsylvania, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Gadue","sequence":"additional","affiliation":[{"name":"Center for Cellular and Molecular Therapeutics, Department of Pathology and Laboratory Medicine,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben Z.","family":"Stanger","sequence":"additional","affiliation":[{"name":"Gastroenterology Division, Department of Medicine,"},{"name":"Department of Cell and Developmental Biology, and"},{"name":"Abramson Family Cancer Research Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"232","published-online":{"date-parts":[[2019,11,1]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2016.105"},{"key":"B2","unstructured":"CDC. National Diabetes Statistics Report 2017. http:\/\/www.cdc.gov\/features\/diabetes-statistic-report\/index.html Updated August 8, 2017. Accessed September 3, 2019"},{"key":"B3","unstructured":"International Diabetes Federation. Diabetes Atlas 2017. 8th ed. http:\/\/diabetesatlas.org\/IDF_Diabetes_Atlas_8e_interactive_EN Accessed September 3, 2019"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.13458"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1900\/RDS.2012.9.385"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200007273430401"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2016.178"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2014.02.013"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2012.02.024"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1393"},{"issue":"4","key":"B11","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1038\/ng.2215","article-title":"Generation of functional insulin-producing cells in the gut by Foxo1 ablation","volume":"44","author":"Talchai","year":"2012","journal-title":"Nat Genet"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(80)90371-6"},{"issue":"1 pt 1","key":"B13","first-page":"E43","article-title":"Relation between blood flow and morphology in islet organ of rat pancreas","volume":"249","author":"Lifson","year":"1985","journal-title":"Am J Physiol"},{"issue":"1","key":"B14","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3727\/096368909788237195","article-title":"Extracellular matrix in pancreatic islets: relevance to scaffold design and transplantation","volume":"18","author":"Stendahl","year":"2009","journal-title":"Cell Transplant"},{"key":"B15","doi-asserted-by":"publisher","DOI":"10.3727\/096368912X659880"},{"key":"B16","doi-asserted-by":"publisher","DOI":"10.3727\/096368910X515872"},{"key":"B17","doi-asserted-by":"publisher","DOI":"10.1385\/ENDO:13:3:251"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1038\/nature12354"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1172\/JCI58447"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199510270-00016"},{"issue":"3","key":"B21","article-title":"Purified human pancreatic islets, CIT culture media: a standard operating procedure of the NIH Clinical Islet Transplantation Consortium","volume":"2","author":"NIH CIT Consortium Chemistry Manufacturing Controls Monitoring Committee","year":"2014","journal-title":"CellR4 Repair Replace Regen Reprogram"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199804270-00009"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0b013e3181d154ac"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.3727\/096368915X688119"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1007\/s00268-006-0719-8"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1002\/jmri.24572"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1007\/s00261-014-0315-6"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2012.06.039"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.04.064"},{"key":"B30","doi-asserted-by":"publisher","DOI":"10.2337\/db06-0779"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.3727\/096368913X673469"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0b013e318293b7b8"},{"key":"B33","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199807150-00004"},{"key":"B34","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2016.01.003"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.09.040"},{"key":"B36","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3033"},{"key":"B37","first-page":"303","article-title":"Long-term culture and maintenance of human islets of Langerhans in memphis serum-free media","volume":"107","author":"Fraga","year":"2005","journal-title":"Methods Mol Med"},{"issue":"3 suppl","key":"B38","first-page":"49","article-title":"Advances in long-term islet culture: the Memphis experience","volume":"40","author":"Gaber","year":"2004","journal-title":"Cell Biochem Biophys"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2004.04.055"}],"container-title":["JCI Insight"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/insight.jci.org\/articles\/view\/124644\/files\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T12:22:00Z","timestamp":1779193320000},"score":1,"resource":{"primary":{"URL":"https:\/\/insight.jci.org\/articles\/view\/124644"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11,1]]},"references-count":39,"journal-issue":{"issue":"21","published-online":{"date-parts":[[2019,11,1]]},"published-print":{"date-parts":[[2019,11,1]]}},"alternative-id":["10.1172\/jci.insight.124644"],"URL":"https:\/\/doi.org\/10.1172\/jci.insight.124644","relation":{},"ISSN":["2379-3708"],"issn-type":[{"value":"2379-3708","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,11,1]]},"article-number":"e124644"}}