{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T12:42:51Z","timestamp":1793277771367,"version":"4.5.0"},"reference-count":56,"publisher":"American Society for Clinical Investigation","issue":"5","funder":[{"name":"NIH","award":["EY026260,HL121036,HL141857,DE027891,DK114645"],"award-info":[{"award-number":["EY026260,HL121036,HL141857,DE027891,DK114645"]}]},{"name":"NIH","award":["HL131652"],"award-info":[{"award-number":["HL131652"]}]},{"name":"NIH","award":["HL121191"],"award-info":[{"award-number":["HL121191"]}]},{"name":"Korean Research Foundation","award":["NRF-2018R1A2A1A05019550"],"award-info":[{"award-number":["NRF-2018R1A2A1A05019550"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,3,7]]},"abstract":"<jats:p>\n                    The lymphatic system plays crucial roles in tissue homeostasis, lipid absorption, and immune cell trafficking. Although lymphatic valves ensure unidirectional lymph flows, the flow itself controls lymphatic valve formation. Here, we demonstrate that a mechanically activated ion channel Piezo1 senses oscillating shear stress (OSS) and incorporates the signal into the genetic program controlling lymphatic valve development and maintenance. Time-controlled deletion of\n                    <jats:italic>Piezo1<\/jats:italic>\n                    using a pan-endothelial Cre driver (\n                    <jats:italic>\n                      Cdh5[PAC]-CreER\n                      <jats:sup>T2<\/jats:sup>\n                    <\/jats:italic>\n                    ) or lymphatic-specific Cre driver (\n                    <jats:italic>\n                      Prox1-CreER\n                      <jats:sup>T2<\/jats:sup>\n                    <\/jats:italic>\n                    ) equally inhibited lymphatic valve formation in newborn mice. Furthermore,\n                    <jats:italic>Piezo1<\/jats:italic>\n                    deletion in adult lymphatics caused substantial lymphatic valve degeneration.\n                    <jats:italic>Piezo1<\/jats:italic>\n                    knockdown in cultured lymphatic endothelial cells (LECs) largely abrogated the OSS-induced upregulation of the lymphatic valve signature genes. Conversely, ectopic Piezo1 overexpression upregulated the lymphatic valve genes in the absence of OSS. Remarkably, activation of Piezo1 using chemical agonist Yoda1 not only accelerated lymphatic valve formation in animals, but also triggered upregulation of some lymphatic valve genes in cultured LECs without exposure to OSS. In summary, our studies together demonstrate that Piezo1 is the force sensor in the mechanotransduction pathway controlling lymphatic valve development and maintenance, and Piezo1 activation is a potentially novel therapeutic strategy for congenital and surgery-associated lymphedema.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.125068","type":"journal-article","created":{"date-parts":[[2019,1,24]],"date-time":"2019-01-24T12:01:33Z","timestamp":1548331293000},"source":"Crossref","is-referenced-by-count":175,"title":["Piezo1 incorporates mechanical force signals into the genetic program that governs lymphatic valve development and maintenance"],"prefix":"10.1172","volume":"4","author":[{"given":"Dongwon","family":"Choi","sequence":"first","affiliation":[{"name":"Department of Surgery, and"},{"name":"Department of Biochemistry and Molecular Medicine, Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, California, 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