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In the present study, ATP-binding cassette (ABC) transporter\u2013linked secretion of human EGF from probiotic\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    (EGF-EcN) was created to promote beneficial actions of the EGF receptor, which is notably attenuated in patients with intestinal ulcerative injuries. Preventive and postinjury treatment with EGF-EcN alleviated intestinal ulcers and other readouts of disease severity in murine intestinal ulcer models. EGF-EcN administration promoted the restitutive recovery of damaged epithelial layers, particularly via upward expansion of highly proliferating progenitor cells from the lower crypts. Along with the epithelial barrier benefit, EGF-EcN improved goblet cell\u2013associated mucosal integrity, which controls the access of luminal microbiota to the underlying host tissues. Despite concern about the oncogenic action of EGF, EGF-EcN did not aggravate colitis-associated colon cancer; instead, it alleviated protumorigenic activities and improved barrier integrity in the lesions. All findings indicate that probiotic bacteria\u2013based precision delivery of human EGF is a promising mucosal intervention against gastrointestinal ulcers and malignant distress through crypt-derived barrier restoration.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.125166","type":"journal-article","created":{"date-parts":[[2019,8,21]],"date-time":"2019-08-21T11:01:11Z","timestamp":1566385271000},"source":"Crossref","is-referenced-by-count":47,"title":["Nononcogenic restoration of the intestinal barrier by E. coli\u2013delivered human EGF"],"prefix":"10.1172","volume":"4","author":[{"given":"Mira","family":"Yu","sequence":"first","affiliation":[{"name":"Laboratory of Mucosal Exposome and Biomodulation, Department of Biomedical Sciences, Biomedical Research Institute, Pusan National University, Yangsan, South Korea."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juil","family":"Kim","sequence":"additional","affiliation":[{"name":"Laboratory of Mucosal 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