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Its soluble guanylate cyclase\u2013independent (sGC-independent) effects are stereoselective \u2014 that is, not recapitulated by\n                    <jats:italic>S<\/jats:italic>\n                    -nitroso-d-cysteine (D-CSNO) \u2014 and are inhibited by chemical congeners. However, candidate L-CSNO receptors have not been identified. Here, we have used 2 complementary affinity chromatography assays \u2014 followed by unbiased proteomic analysis \u2014 to identify voltage-gated K\n                    <jats:sup>+<\/jats:sup>\n                    channel (Kv) proteins as binding partners for L-CSNO. Stereoselective L-CSNO\u2013Kv interaction was confirmed structurally and functionally using surface plasmon resonance spectroscopy; hydrogen deuterium exchange; and, in Kv1.1\/Kv1.2\/Kv\u03b22-overexpressing cells, patch clamp assays. Remarkably, these sGC-independent L-CSNO effects did not involve\n                    <jats:italic>S<\/jats:italic>\n                    -nitrosylation of Kv proteins. In isolated rat and mouse respiratory control (petrosyl) ganglia, L-CSNO stereoselectively inhibited Kv channel function. Genetic ablation of Kv1.1 prevented this effect. In intact animals, L-CSNO injection at the level of the carotid body dramatically and stereoselectively increased minute ventilation while having no effect on blood pressure; this effect was inhibited by the L-CSNO congener\n                    <jats:italic>S<\/jats:italic>\n                    -methyl-l-cysteine. Kv proteins are physiologically relevant targets of endogenous L-CSNO. This may be a signaling pathway of broad relevance.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.134174","type":"journal-article","created":{"date-parts":[[2020,8,13]],"date-time":"2020-08-13T12:04:12Z","timestamp":1597320252000},"source":"Crossref","is-referenced-by-count":29,"title":["Voltage-gated potassium channel proteins and stereoselective S-nitroso-l-cysteine signaling"],"prefix":"10.1172","volume":"5","author":[{"given":"Benjamin","family":"Gaston","sequence":"first","affiliation":[{"name":"Riley Hospital for Children, Indianapolis, Indiana, USA."},{"name":"Department of Pediatric Pulmonology,"},{"name":"Department of Physiology and Biophysics,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Pediatric 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