{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T09:20:01Z","timestamp":1790760001518,"version":"4.1.0"},"reference-count":79,"publisher":"American Society for Clinical Investigation","issue":"11","license":[{"start":{"date-parts":[[2020,6,4]],"date-time":"2020-06-04T00:00:00Z","timestamp":1591228800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004442","name":"National Science Centre","doi-asserted-by":"crossref","award":["MAESTRO 3 -2012\/06\/A\/NZ1\/00004 to JD"],"award-info":[{"award-number":["MAESTRO 3 -2012\/06\/A\/NZ1\/00004 to JD"]}],"id":[{"id":"10.13039\/501100004442","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004442","name":"National Science Centre","doi-asserted-by":"crossref","award":["MAESTRO 10 - 2018\/30\/A\/NZ3\/00412 to JD"],"award-info":[{"award-number":["MAESTRO 10 - 2018\/30\/A\/NZ3\/00412 to JD"]}],"id":[{"id":"10.13039\/501100004442","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004442","name":"National Science Centre","doi-asserted-by":"crossref","award":["OPUS 11 - 2016\/21\/B\/NZ1\/00293 to A<0141>"],"award-info":[{"award-number":["OPUS 11 - 2016\/21\/B\/NZ1\/00293 to A<0141>"]}],"id":[{"id":"10.13039\/501100004442","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004442","name":"National Science Centre","doi-asserted-by":"crossref","award":["PRELUDIUM 16 - 2018\/31\/N\/NZ4\/02500 to PP"],"award-info":[{"award-number":["PRELUDIUM 16 - 2018\/31\/N\/NZ4\/02500 to PP"]}],"id":[{"id":"10.13039\/501100004442","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,6,4]]},"abstract":"<jats:p>\n                    The severity of Duchenne muscular dystrophy (DMD), an incurable disease caused by the lack of dystrophin, might be modulated by different factors, including miRNAs. Among them, miR-378 is considered of high importance for muscle biology, but intriguingly, its role in DMD and its murine model (\n                    <jats:italic>mdx<\/jats:italic>\n                    mice) has not been thoroughly addressed so far. Here, we demonstrate that dystrophic mice additionally globally lacking miR-378 (double-KO [dKO] animals) exhibited better physical performance and improved absolute muscle force compared with\n                    <jats:italic>mdx<\/jats:italic>\n                    mice. Accordingly, markers of muscle damage in serum were significantly decreased in dKO mice, accompanied by diminished inflammation, fibrosis, and reduced abundance of regenerating fibers within muscles. The lack of miR-378 also normalized the aggravated fusion of dystrophin-deficient muscle satellite cells (mSCs). RNA sequencing of gastrocnemius muscle transcriptome revealed fibroblast growth factor 1 (\n                    <jats:italic>Fgf1<\/jats:italic>\n                    ) as one of the most significantly downregulated genes in mice devoid of miR-378, indicating FGF1 as one of the mediators of changes driven by the lack of miR-378. In conclusion, we suggest that targeting miR-378 has the potential to ameliorate DMD pathology.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.135576","type":"journal-article","created":{"date-parts":[[2020,6,9]],"date-time":"2020-06-09T08:24:50Z","timestamp":1591691090000},"source":"Crossref","is-referenced-by-count":33,"title":["Lack of miR-378 attenuates muscular dystrophy in mdx mice"],"prefix":"10.1172","volume":"5","author":[{"given":"Paulina","family":"Podkalicka","sequence":"first","affiliation":[{"name":"Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5612-4112","authenticated-orcid":true,"given":"Olga","family":"Mucha","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iwona","family":"Bronisz-Budzy\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Kozakowska","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Pietraszek-Gremplewicz","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Cetnarowska","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Urszula","family":"G\u0142owniak-Kwitek","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9181-439X","authenticated-orcid":true,"given":"Karolina","family":"Bukowska-Strakova","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, and"},{"name":"Department of Clinical Immunology and Transplantology, Institute of Pediatrics, Medical College, Jagiellonian University, Krakow, Poland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8460-1991","authenticated-orcid":true,"given":"Maciej","family":"Cie\u015bla","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9830-8892","authenticated-orcid":true,"given":"Maria","family":"Kulecka","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hepatology and Clinical Oncology, Centre of Postgraduate Medical Education, Warsaw, Poland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1363-3766","authenticated-orcid":true,"given":"Jerzy","family":"Ostrowski","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hepatology and Clinical Oncology, Centre of Postgraduate Medical Education, Warsaw, Poland."},{"name":"Department of Genetics, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micha\u0142","family":"Miku\u0142a","sequence":"additional","affiliation":[{"name":"Department of Genetics, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Potulska-Chromik","sequence":"additional","affiliation":[{"name":"Department of Neurology, Medical University of Warsaw, Warsaw, Poland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7309-1914","authenticated-orcid":true,"given":"Anna","family":"Kostera-Pruszczyk","sequence":"additional","affiliation":[{"name":"Department of Neurology, Medical University of Warsaw, Warsaw, Poland."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicja","family":"J\u00f3zkowicz","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3523-3560","authenticated-orcid":true,"given":"Agnieszka","family":"\u0141oboda","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5687-0839","authenticated-orcid":true,"given":"J\u00f3zef","family":"Dulak","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"232","published-online":{"date-parts":[[2020,6,4]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)07815-7"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.23528"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90579-4"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1002\/humu.22758"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aaa7322"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1242\/dev.069088"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3990"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2016.04.002"},{"key":"B9","doi-asserted-by":"crossref","DOI":"10.1155\/2014\/965631","article-title":"Understanding the process of fibrosis in Duchenne muscular dystrophy","volume":"2014","author":"Kharraz","year":"2014","journal-title":"Biomed Res Int"},{"key":"B10","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(18)30024-3"},{"issue":"2","key":"B11","first-page":"121","article-title":"Improvement of survival in Duchenne Muscular Dystrophy: retrospective analysis of 835 patients","volume":"31","author":"Passamano","year":"2012","journal-title":"Acta Myol"},{"key":"B12","doi-asserted-by":"publisher","DOI":"10.2147\/NDT.S93873"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2017.7435"},{"key":"B14","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2011.3964"},{"key":"B15","doi-asserted-by":"crossref","DOI":"10.1038\/mtna.2014.29","article-title":"Circulating Muscle-specific miRNAs in Duchenne Muscular Dystrophy Patients","volume":"3","author":"Li","year":"2014","journal-title":"Mol Ther Nucleic Acids"},{"issue":"1","key":"B16","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1111\/j.1582-4934.2008.00524.x","article-title":"miRNAS in normal and diseased skeletal muscle","volume":"13","author":"Eisenberg","year":"2009","journal-title":"J Cell Mol Med"},{"key":"B17","doi-asserted-by":"crossref","unstructured":"Krist B, et al. miR-378a influences vascularization in skeletal muscles [published ahead of print August 28, 2019]. Cardiovasc Res. https:\/\/doi.org\/10.1093\/cvr\/cvz236","DOI":"10.1093\/cvr\/cvz236"},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1803377115"},{"key":"B19","doi-asserted-by":"crossref","DOI":"10.1155\/2015\/281756","article-title":"The Role of miR-378a in Metabolism, Angiogenesis, and Muscle Biology","volume":"2015","author":"Krist","year":"2015","journal-title":"Int J Endocrinol"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.219006"},{"key":"B21","doi-asserted-by":"crossref","DOI":"10.1038\/mtna.2012.26","article-title":"Expression analysis in multiple muscle groups and serum reveals complexity in the microRNA transcriptome of the mdx mouse with implications for therapy","volume":"1","author":"Roberts","year":"2012","journal-title":"Mol Ther Nucleic Acids"},{"key":"B22","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0055281"},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt724"},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1093\/abbs\/gmw077"},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.139.2.375"},{"key":"B26","doi-asserted-by":"publisher","DOI":"10.1111\/febs.12166"},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2013.02.003"},{"key":"B28","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddn129"},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65050-X"},{"issue":"3","key":"B30","first-page":"184","article-title":"The role of fibrosis in Duchenne muscular dystrophy","volume":"31","author":"Klingler","year":"2012","journal-title":"Acta Myol"},{"key":"B31","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-06068-6"},{"key":"B32","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00043.2011"},{"key":"B33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2014\/612471","article-title":"The Basis of Muscle Regeneration","volume":"612471","author":"Musar\u00f2","year":"2014","journal-title":"Advances in Biology"},{"key":"B34","doi-asserted-by":"crossref","DOI":"10.1186\/s13395-015-0046-6","article-title":"Developmental myosins: expression patterns and functional significance","volume":"5","author":"Schiaffino","year":"2015","journal-title":"Skelet Muscle"},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.253534"},{"key":"B36","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0000116","article-title":"MiRNA-directed regulation of VEGF and other angiogenic factors under hypoxia","volume":"1","author":"Hua","year":"2006","journal-title":"PLoS One"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00031.2010"},{"key":"B38","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00993.2010"},{"key":"B39","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddu287"},{"key":"B40","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.06.051"},{"key":"B41","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.489674"},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1038\/352536a0"},{"key":"B43","doi-asserted-by":"publisher","DOI":"10.3233\/JND-150106"},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1999.277.6.C1170"},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.1093\/ejo\/cjq113"},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1007\/s004010050965"},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(88)90463-1"},{"key":"B48","doi-asserted-by":"publisher","DOI":"10.1186\/s13395-019-0207-0"},{"key":"B49","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-019-03006-7"},{"key":"B50","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1207605109"},{"issue":"1","key":"B51","doi-asserted-by":"crossref","DOI":"10.1016\/j.pmr.2011.11.014","article-title":"The paradox of muscle hypertrophy in muscular dystrophy","volume":"23","author":"Kornegay","year":"2012","journal-title":"Phys Med Rehabil Clin N Am"},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2018.08.026"},{"key":"B53","doi-asserted-by":"publisher","DOI":"10.1159\/000362957"},{"key":"B54","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-02-408252"},{"key":"B55","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2015.00602","article-title":"Macrophages During the Fibrotic Process: M2 as Friend and Foe","volume":"6","author":"Braga","year":"2015","journal-title":"Front Immunol"},{"key":"B56","doi-asserted-by":"publisher","DOI":"10.7150\/thno.22878"},{"key":"B57","doi-asserted-by":"crossref","DOI":"10.1038\/ncomms10993","article-title":"MicroRNA-378 limits activation of hepatic stellate cells and liver fibrosis by suppressing Gli3 expression","volume":"7","author":"Hyun","year":"2016","journal-title":"Nat Commun"},{"key":"B58","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.02.053"},{"key":"B59","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2016.4764"},{"issue":"2","key":"B60","first-page":"307","article-title":"Embryonic myosin is a regeneration marker to monitor utrophin-based therapies for DMD","volume":"28","author":"Guiraud","year":"2019","journal-title":"Hum Mol Genet"},{"key":"B61","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/10.11.1183"},{"key":"B62","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.105.2.949"},{"key":"B63","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp550"},{"key":"B64","doi-asserted-by":"publisher","DOI":"10.3109\/08977199909103519"},{"key":"B65","doi-asserted-by":"crossref","DOI":"10.1186\/s12931-015-0242-2","article-title":"Increased FGF1-FGFRc expression in idiopathic pulmonary fibrosis","volume":"16","author":"MacKenzie","year":"2015","journal-title":"Respir Res"},{"key":"B66","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2013.03.003"},{"key":"B67","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddx150"},{"key":"B68","doi-asserted-by":"crossref","DOI":"10.3389\/fgene.2018.00114","article-title":"Combined Therapies for Duchenne Muscular Dystrophy to Optimize Treatment Efficacy","volume":"9","author":"Cordova","year":"2018","journal-title":"Front Genet"},{"key":"B69","doi-asserted-by":"crossref","DOI":"10.1155\/2010\/724914","article-title":"Impaired skeletal muscle repair after ischemia-reperfusion injury in mice","volume":"2010","author":"Vignaud","year":"2010","journal-title":"J Biomed Biotechnol"},{"key":"B70","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2011.01.054"},{"key":"B71","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2017.10.017"},{"key":"B72","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmet.2013.10.005"},{"issue":"1","key":"B73","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/0003-2697(87)90021-2","article-title":"Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction","volume":"162","author":"Chomczynski","year":"1987","journal-title":"Anal Biochem"},{"key":"B74","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0031278"},{"key":"B75","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts635"},{"key":"B76","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1923"},{"key":"B77","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu638"},{"key":"B78","doi-asserted-by":"publisher","DOI":"10.1089\/omi.2011.0118"},{"key":"B79","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt285"}],"container-title":["JCI Insight"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/insight.jci.org\/articles\/view\/135576\/files\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T12:13:54Z","timestamp":1779192834000},"score":1,"resource":{"primary":{"URL":"https:\/\/insight.jci.org\/articles\/view\/135576"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,4]]},"references-count":79,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2020,6,4]]},"published-print":{"date-parts":[[2020,6,4]]}},"alternative-id":["10.1172\/jci.insight.135576"],"URL":"https:\/\/doi.org\/10.1172\/jci.insight.135576","relation":{},"ISSN":["2379-3708"],"issn-type":[{"value":"2379-3708","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,6,4]]},"article-number":"e135576"}}