{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:59:06Z","timestamp":1787018346585,"version":"build-2736575974"},"reference-count":46,"publisher":"American Society for Clinical Investigation","issue":"15","funder":[{"name":"JSPS KAKENHI Grant","award":["#JP19K17880"],"award-info":[{"award-number":["#JP19K17880"]}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["19bm0804004h0203"],"award-info":[{"award-number":["19bm0804004h0203"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,8,6]]},"abstract":"<jats:p>\n                    M3 muscarinic acetylcholine receptor (M3R) is one of the autoantigens associated with Sj\u00f6gren\u2019s syndrome (SS) and is localized in exocrine glands where disease-specific inflammation occurs. The inflammatory lesion is characterized by infiltration of CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells, including clonally expanded Th17 cells. We undertook this study to identify circulating M3R-specific Th17 cells and to determine functional properties of those cells. Using the enzyme-linked immunospot assay (ELISpot) method, we identified M3R-reactive Th17 cells in the peripheral blood of patients with primary SS (pSS). Among 10 examined pSS patients, 10 healthy subjects (HS), and 5 IgG4-related disease (IgG4-RD) patients, M3R-reactive IL-17 secreting cells were significantly increased in 5 pSS patients specifically. The most common T cell epitope, which was analyzed and confirmed by coculture of isolated CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells with antigen presenting cells plus M3R peptides in vitro, was peptide 83-95 of M3R. Peptide recognition was partly in an HLA-DR\u2013restricted manner, confirmed by blocking assay. M3R-reactive Th17 cells positivity correlated with higher titers of anti-M3R antibodies, whose systemic disease activity score tended to be higher. Our studies highlight the role of tissue-specific autoantigen\u2013derived circulating Th17 cells in pSS, for which further work might lead to antigen-specific targeted therapy.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.135982","type":"journal-article","created":{"date-parts":[[2020,7,2]],"date-time":"2020-07-02T12:01:14Z","timestamp":1593691274000},"source":"Crossref","is-referenced-by-count":19,"title":["M3 muscarinic acetylcholine receptor\u2013reactive Th17 cells in primary Sj\u00f6gren\u2019s syndrome"],"prefix":"10.1172","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2793-5589","authenticated-orcid":true,"given":"Saori","family":"Abe","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Faculty of Medicine, University of Tsukuba, Ibaraki, 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