{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T02:55:31Z","timestamp":1792810531279,"version":"4.5.0"},"reference-count":57,"publisher":"American Society for Clinical Investigation","issue":"19","license":[{"start":{"date-parts":[[2020,10,2]],"date-time":"2020-10-02T00:00:00Z","timestamp":1601596800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"crossref","award":["n\/a"],"award-info":[{"award-number":["n\/a"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Centers of Biomedical Research Excellence","award":["1P20GM130459"],"award-info":[{"award-number":["1P20GM130459"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,10,2]]},"abstract":"<jats:p>\n                    Seizures can result in a severe hypoperfusion\/hypoxic attack that causes postictal memory and behavioral impairments. However, neither postictal changes to microvasculature nor Ca\n                    <jats:sup>2+<\/jats:sup>\n                    changes in key cell types controlling blood perfusion have been visualized in vivo, leaving essential components of the underlying cellular mechanisms unclear. Here, we use 2-photon microvascular and Ca\n                    <jats:sup>2+<\/jats:sup>\n                    imaging in awake mice to show that seizures result in a robust vasoconstriction of cortical penetrating arterioles, which temporally mirrors the prolonged postictal hypoxia. The vascular effect was dependent on cyclooxygenase 2, as pretreatment with ibuprofen prevented postictal vasoconstriction. Moreover, seizures caused a rapid elevation in astrocyte endfoot Ca\n                    <jats:sup>2+<\/jats:sup>\n                    that was confined to the seizure period, and vascular smooth muscle cells displayed a significant increase in Ca\n                    <jats:sup>2+<\/jats:sup>\n                    both during and following seizures, lasting up to 75 minutes. Our data show enduring postictal vasoconstriction and temporal activities of 2 cell types within the neurovascular unit that are associated with seizure-induced hypoperfusion\/hypoxia. These findings support prevention of this event may be a novel and tractable treatment strategy in patients with epilepsy who experience extended postseizure impairments.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.136469","type":"journal-article","created":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T11:01:40Z","timestamp":1601550100000},"source":"Crossref","is-referenced-by-count":43,"title":["Seizures elevate gliovascular unit Ca2+ and cause sustained vasoconstriction"],"prefix":"10.1172","volume":"5","author":[{"given":"Cam Ha T.","family":"Tran","sequence":"first","affiliation":[{"name":"Hotchkiss Brain Institute and"},{"name":"Department of Physiology and Pharmacology, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada."},{"name":"Department of Physiology and Cell Biology, University of Nevada, Reno School of Medicine, Reno, Nevada, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antis G.","family":"George","sequence":"additional","affiliation":[{"name":"Hotchkiss Brain Institute and"},{"name":"Department of Cell Biology and Anatomy, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8462-355X","authenticated-orcid":true,"given":"G. 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