{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:33:28Z","timestamp":1792787608213,"version":"4.5.0"},"reference-count":46,"publisher":"American Society for Clinical Investigation","issue":"7","license":[{"start":{"date-parts":[[2021,4,8]],"date-time":"2021-04-08T00:00:00Z","timestamp":1617840000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003196","name":"Italian Ministry of Health","doi-asserted-by":"crossref","award":["GR-2013- 02356592"],"award-info":[{"award-number":["GR-2013- 02356592"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Roche per la ricerca","award":["Roche per la Ricerca 2019"],"award-info":[{"award-number":["Roche per la Ricerca 2019"]}]},{"name":"NIH\/NIAMS","award":["R01AR076247-01"],"award-info":[{"award-number":["R01AR076247-01"]}]},{"name":"MDA","award":["418870"],"award-info":[{"award-number":["418870"]}]},{"name":"NIH","award":["R01AR064873"],"award-info":[{"award-number":["R01AR064873"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,4,8]]},"abstract":"<jats:p>\n                    Here, we report on the identification of Itga7-expressing muscle-resident glial cells activated by loss of neuromuscular junction (NMJ) integrity. Gene expression analysis at the bulk and single-cell level revealed that these cells are distinct from Itga7-expressing muscle satellite cells. We show that a selective activation and expansion of Itga7\n                    <jats:sup>+<\/jats:sup>\n                    glial cells occur in response to muscle nerve lesion. Upon activation, muscle glial\u2013derived progenies expressed neurotrophic genes, including nerve growth factor receptor, which enables their isolation by FACS. We show that activated muscle glial cells also expressed genes potentially implicated in extracellular matrix remodeling at NMJs. We found that tenascin C, which was highly expressed by muscle glial cells, activated upon nerve injury and preferentially localized to NMJ. Interestingly, we observed that the activation of muscle glial cells by acute nerve injury was reversible upon NMJ repair. By contrast, in a mouse model of ALS, in which NMJ degeneration is progressive, muscle glial cells steadily increased over the course of the disease. However, they exhibited an impaired neurotrophic activity, suggesting that pathogenic activation of glial cells may be implicated in ALS progression.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.143469","type":"journal-article","created":{"date-parts":[[2021,3,4]],"date-time":"2021-03-04T12:01:19Z","timestamp":1614859279000},"source":"Crossref","is-referenced-by-count":42,"title":["Activation of skeletal muscle\u2013resident glial cells upon nerve injury"],"prefix":"10.1172","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6292-8849","authenticated-orcid":true,"given":"Daisy","family":"Proietti","sequence":"first","affiliation":[{"name":"IRCCS Fondazione Santa Lucia, Rome, Italy."},{"name":"Department of Anatomy, Histology, Forensic Medicine and Orthopedics, University of Rome \u201cla Sapienza\u201d, Rome, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorenzo","family":"Giordani","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM UMRS 974, Association Institut de Myologie, Centre de Recherche en Myologie, Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8217-970X","authenticated-orcid":true,"given":"Marco","family":"De Bardi","sequence":"additional","affiliation":[{"name":"IRCCS Fondazione Santa Lucia, Rome, Italy."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"D\u2019Ercole","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology, Forensic Medicine and Orthopedics, University of Rome \u201cla Sapienza\u201d, Rome, 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