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Deep TCR V\u03b2 sequencing of 135 serial specimens from a cohort of 35 allo-HCT recipients\/donors was performed to dissect posttransplant TCR architecture and dynamics. Paired analysis of clonotypic repertoires showed a minimal overlap with donor expansions. Rarefied and hyperexpanded clonotypic patterns were hallmarks of T cell reconstitution and influenced clinical outcomes. Donor and pretransplant TCR diversity as well as divergence of class I human leukocyte antigen genotypes were major predictors of recipient TCR repertoire recovery. Complementary determining region 3\u2013based specificity spectrum analysis indicated a predominant expansion of pathogen- and tumor-associated clonotypes in the late post\u2013allo-HCT phase, while autoreactive clones were more expanded in the case of graft-versus-host disease occurrence. 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