{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T16:18:10Z","timestamp":1787501890051,"version":"build-2736575974"},"reference-count":31,"publisher":"American Society for Clinical Investigation","issue":"16","license":[{"start":{"date-parts":[[2021,8,23]],"date-time":"2021-08-23T00:00:00Z","timestamp":1629676800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Cancer Institute","award":["P50 CA171963"],"award-info":[{"award-number":["P50 CA171963"]}]},{"name":"National Cancer Institute","award":["R35 CA210084"],"award-info":[{"award-number":["R35 CA210084"]}]},{"name":"National Cancer Institute","award":["P01 CA101937"],"award-info":[{"award-number":["P01 CA101937"]}]},{"DOI":"10.13039\/100001445","name":"Alex\u2019s Lemonade Stand Foundation for Childhood Cancer","doi-asserted-by":"crossref","award":["Young Investigator Award"],"award-info":[{"award-number":["Young Investigator Award"]}],"id":[{"id":"10.13039\/100001445","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Cancer Institute","award":["SPORE in Leukemia Career Enhancement Program"],"award-info":[{"award-number":["SPORE in Leukemia Career Enhancement Program"]}]},{"DOI":"10.13039\/100000861","name":"Burroughs Wellcome Fund","doi-asserted-by":"crossref","award":["Dean\u2019s Scholars Award from the Washington University Division of Physician Scientists"],"award-info":[{"award-number":["Dean\u2019s Scholars Award from the Washington University Division of Physician Scientists"]}],"id":[{"id":"10.13039\/100000861","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,8,23]]},"abstract":"<jats:p>\n                    Targeting T cell malignancies with universal CD7-targeting chimeric antigen receptor T cells (UCART7) can lead to profound immune deficiency due to loss of normal T and NK cells. While a small population of endogenous CD7\n                    <jats:sup>\u2013<\/jats:sup>\n                    T cells exists, these cells are unlikely to be able to repopulate the entire immune repertoire after UCART7 treatment, as they are limited in number and proliferative capacity. To rescue T and NK cells after UCART7, we created hematopoietic stem cells genetically deleted for\n                    <jats:italic>CD7<\/jats:italic>\n                    (CD7-KO HSCs). CD7-KO HSCs were able to engraft immunodeficient mice and differentiate into T and NK cells lacking CD7 expression. CD7-KO T and NK cells could perform effector functions as robustly as control T and NK cells. Furthermore, CD7-KO T cells were phenotypically and functionally distinct from endogenous CD7\n                    <jats:sup>\u2013<\/jats:sup>\n                    T cells, indicating that CD7-KO T cells can supplement immune functions lacking in CD7\n                    <jats:sup>\u2013<\/jats:sup>\n                    T cells. Mice engrafted with CD7-KO HSCs maintained T and NK cell numbers after UCART7 treatment, while these were significantly decreased in control mice. These studies support the development of CD7-KO HSCs to augment host immunity in patients with T cell malignancies after UCART7 treatment.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.149819","type":"journal-article","created":{"date-parts":[[2021,8,23]],"date-time":"2021-08-23T16:37:37Z","timestamp":1629736657000},"source":"Crossref","is-referenced-by-count":37,"title":["CD7-deleted hematopoietic stem cells can restore immunity after CAR T cell therapy"],"prefix":"10.1172","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9609-0685","authenticated-orcid":true,"given":"Miriam Y.","family":"Kim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew L.","family":"Cooper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8831-7592","authenticated-orcid":true,"given":"Miriam T.","family":"Jacobs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie K.","family":"Ritchey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Hollaway","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Todd A.","family":"Fehniger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John F.","family":"DiPersio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"232","published-online":{"date-parts":[[2021,8,23]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.05.013"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-018-0277-8"},{"issue":"24","key":"B3","doi-asserted-by":"crossref","first-page":"11978","DOI":"10.1073\/pnas.1819992116","article-title":"Gene-edited stem cells enable CD33-directed immune therapy for myeloid malignancies","volume":"116","author":"Borot","year":"2019","journal-title":"Proc Natl Acad Sci U S A"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-018-0065-5"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2017-01-761320"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2017009928"},{"issue":"2","key":"B7","doi-asserted-by":"crossref","first-page":"517","DOI":"10.4049\/jimmunol.152.2.517","article-title":"Expression and function of CD7 molecule on human natural killer cells","volume":"152","author":"Rabinowich","year":"1994","journal-title":"J Immunol"},{"issue":"5","key":"B8","doi-asserted-by":"crossref","first-page":"2081","DOI":"10.4049\/jimmunol.150.5.2081","article-title":"CD7- T cells represent a subset of normal human blood lymphocytes","volume":"150","author":"Reinhold","year":"1993","journal-title":"J Immunol"},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1907883116"},{"key":"B10","doi-asserted-by":"crossref","unstructured":"Ramaswamy A, et al. 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