{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:59:08Z","timestamp":1787018348211,"version":"build-2736575974"},"reference-count":67,"publisher":"American Society for Clinical Investigation","issue":"1","license":[{"start":{"date-parts":[[2022,1,11]],"date-time":"2022-01-11T00:00:00Z","timestamp":1641859200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01HL148355,R01HL115141,R01HL148207,R01HL153356"],"award-info":[{"award-number":["R01HL148355,R01HL115141,R01HL148207,R01HL153356"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"crossref","award":["18SFRN33900144,20SFRN35110004"],"award-info":[{"award-number":["18SFRN33900144,20SFRN35110004"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,1,11]]},"abstract":"<jats:p>\n                    <jats:italic>SNHG12<\/jats:italic>\n                    , a long noncoding RNA (lncRNA) dysregulated in atherosclerosis, is known to be a key regulator of vascular senescence in endothelial cells (ECs). However, its role in angiogenesis and peripheral artery disease has not been elucidated. Hind-limb ischemia studies using femoral artery ligation (FAL) in mice showed that\n                    <jats:italic>SNHG12<\/jats:italic>\n                    expression falls readily in the acute phase of the response to limb ischemia in gastrocnemius muscle and recovers to normal when blood flow recovery is restored to ischemic muscle, indicating that it likely plays a role in the angiogenic response to ischemia. Gain- and loss-of-function studies demonstrated that\n                    <jats:italic>SNHG12<\/jats:italic>\n                    regulated angiogenesis \u2014\n                    <jats:italic>SNHG12<\/jats:italic>\n                    deficiency reduced cell proliferation, migration, and endothelial sprouting, whereas overexpression promoted these angiogenic functions. We identified\n                    <jats:italic>SNHG12<\/jats:italic>\n                    binding partners by proteomics that may contribute to its role in angiogenesis, including IGF-2 mRNA\u2013binding protein 3 (IGF2BP3, also known as IMP3). RNA-Seq profiling of\n                    <jats:italic>SNHG12<\/jats:italic>\n                    -deficient ECs showed effects on angiogenesis pathways and identified a strong effect on cell cycle regulation, which may be modulated by IMP3. Knockdown of\n                    <jats:italic>SNHG12<\/jats:italic>\n                    in mice undergoing FAL using injected gapmeRs) decreased angiogenesis, an effect that was more pronounced in a model of insulin-resistant\n                    <jats:italic>db\/db<\/jats:italic>\n                    mice. RNA-Seq profiling of the EC and non-EC compartments in these mice revealed a likely role of\n                    <jats:italic>SNHG12<\/jats:italic>\n                    knockdown on Wnt, Notch, and angiopoietin signaling pathways. Together, these findings indicate that\n                    <jats:italic>SNHG12<\/jats:italic>\n                    plays an important role in the angiogenic EC response to ischemia.\n                  <\/jats:p>","DOI":"10.1172\/jci.insight.150761","type":"journal-article","created":{"date-parts":[[2021,11,18]],"date-time":"2021-11-18T12:02:34Z","timestamp":1637236954000},"source":"Crossref","is-referenced-by-count":18,"title":["Deficiency of lncRNA SNHG12 impairs ischemic limb neovascularization by altering an endothelial cell cycle pathway"],"prefix":"10.1172","volume":"7","author":[{"given":"David A.","family":"Gross","sequence":"first","affiliation":[{"name":"Department of Medicine, Cardiovascular Division, Brigham and Women\u2019s Hospital, Harvard Medical School, Boston, Massachusetts, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henry S.","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Medicine, Cardiovascular Division, Brigham and Women\u2019s Hospital, Harvard Medical School, Boston, Massachusetts, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rulin","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Department of Medicine, Cardiovascular Division, Brigham and Women\u2019s Hospital, Harvard Medical School, Boston, Massachusetts, USA."},{"name":"Department of Cardiovascular Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1981-6398","authenticated-orcid":true,"given":"Michael G.","family":"McCoy","sequence":"additional","affiliation":[{"name":"Department of Medicine, Cardiovascular Division, Brigham and Women\u2019s Hospital, Harvard Medical School, Boston, Massachusetts, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0969-8762","authenticated-orcid":true,"given":"Daniel","family":"P\u00e9rez-Cremades","sequence":"additional","affiliation":[{"name":"Department of Medicine, Cardiovascular Division, Brigham and Women\u2019s Hospital, Harvard Medical School, Boston, Massachusetts, USA."},{"name":"Department of Physiology, University of Valencia and INCLIVA Biomedical Research Institute, Valencia, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zachary","family":"Salyers","sequence":"additional","affiliation":[{"name":"Department of Applied Physiology and Kinesiology, University of Florida, Gainesville, Florida, USA."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1468-6053","authenticated-orcid":true,"given":"A.K.M. 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